Superoxide Dismutase from the Silkworm,Bombyx mori: Sequence, Distribution, and Overexpression

2005 ◽  
Vol 69 (3) ◽  
pp. 507-514 ◽  
Author(s):  
Kohji YAMAMOTO ◽  
Pingbo ZHANG ◽  
Yutaka BANNO ◽  
Hiroshi FUJII ◽  
Fumio MIAKE ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Bombyx Mori ◽  
Sequence Distribution ◽  
Silkworm Bombyx Mori

Catalase from the silkworm, Bombyx mori: Gene sequence, distribution, and overexpression

2005 ◽  
Vol 35 (4) ◽  
pp. 277-283 ◽  
Author(s):  
Kohji Yamamoto ◽  
Yutaka Banno ◽  
Hiroshi Fujii ◽  
Fumio Miake ◽  
Nobuhiro Kashige ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Gene Sequence ◽  
Sequence Distribution ◽  
Silkworm Bombyx Mori

scholarly journals Effect of Superoxide Dismutase Gene Inactivation on Virulence of Pseudomonas aeruginosa PAO1 toward the Silkworm, Bombyx mori

2007 ◽  
Vol 73 (5) ◽  
pp. 1569-1575 ◽  
Author(s):  
Kazuhiro Iiyama ◽  
Yuuka Chieda ◽  
Jae Man Lee ◽  
Takahiro Kusakabe ◽  
Chisa Yasunaga-Aoki ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Superoxide Dismutase ◽  
Bombyx Mori ◽  
Double Mutant ◽  
Microscopic Analysis ◽  
Infection Process ◽  
Lag Phase ◽  
Superoxide Dismutase Gene ◽  
Silkworm Bombyx Mori

ABSTRACT To investigate the role of superoxide dismutase (SOD) in virulence against the silkworm, Bombyx mori, mutants of Pseudomonas aeruginosa PAO1 lacking manganese-SOD (PAO1sodM), iron-SOD (PAO1sodB), or both (PAO1sodMB) were generated. The mutants were injected into the hemocoel of B. mori. The virulence decreased in the order PAO1 = PAO1sodM > PAO1sodB > PAO1sodMB. In particular, PAO1sodMB was avirulent at a dose of 105 cells or less. The sod double mutant PAO1sodMB was then complemented with either pSodM or pSodB in trans. In both the complemented strains, the virulence was partially restored. Of the two plasmids, pSodB contributed more to the virulence of P. aeruginosa against B. mori. The results of growth in B. mori hemolymph broth and microscopic analysis suggested that a longer lag phase and superoxide sensitivity correlated with decreased virulence in sod mutants. In conclusion, the SODs are required for full virulence of P. aeruginosa against B. mori and Fe-SOD is more important than Mn-SOD in the infection process.

scholarly journals Characterization of Heat Shock Protein 60 as an Interacting Partner of Superoxide Dismutase 2 in the Silkworm, Bombyx mori, and Its Response to the Molting Hormone, 20-Hydroxyecdysone

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1385
Author(s):  
Yosui Nojima
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Bombyx Mori ◽  
Molecular Mechanisms ◽  
Heat Shock Protein 60 ◽  
Molting Hormone ◽  
Superoxide Dismutase 2 ◽  
Silkworm Bombyx Mori

Oxidative stress promotes pupation in some holometabolous insects. The levels of superoxide, a reactive oxygen species (ROS), are increased and superoxide dismutase 1 (BmSod1) and superoxide dismutase 2 (BmSod2) are decreased during metamorphic events in silkworm (Bombyx mori). These observations strongly suggest that pupation is initiated by oxidative stress via the down-regulation of BmSod1 and BmSod2. However, the molecular mechanisms underlying ROS production during metamorphic events in silkworm remain unknown. To investigate these molecular mechanisms, the peripheral proteins of BmSod1 and BmSod2 were identified and characterized using dry and wet approaches in this study. Based on the results, silkworm heat shock protein 60 (BmHsp60) was identified as an interacting partner of BmSod2, which belongs to the Fe/MnSOD family. Furthermore, the present study results showed that BmHsp60 mRNA expression levels were increased in response to oxidative stress caused by ultraviolet radiation and that BmHsp60 protein levels (but not mRNA levels) were decreased during metamorphic events, which are regulated by the molting hormone 20-hydroxyecdysone. These findings improve our understanding of the mechanisms by which holometabolous insects control ROS during metamorphosis.

Anti-diabetic effects of the silkworm (Bombyx mori.) extracts in the db/db mice

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
KS Ryu ◽  
HS Lee ◽  
KY Kim ◽  
MJ Kim ◽  
PD Kang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silkworm Bombyx Mori

Silkworm Bombyx Mori – An Economic Insect

2012 ◽  
Vol 2 (7) ◽  
pp. 535-537 ◽  
Author(s):  
G. SAVITHRI G. SAVITHRI ◽  
◽  
P. SUJATHAMMA P. SUJATHAMMA ◽  
V.Asha Krishna
Keyword(s):  
Bombyx Mori ◽  
Silkworm Bombyx Mori
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