Potential of Pseudomonas Aeruginosa to Control Sclerotium Rolfsii Causing Stem Rot and Collar Rot Disease of Tomato

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Natedara Chanutsa ◽  
Nutchanat Phonkerd ◽  
Wandee Bunyatratchata
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Collar Rot ◽  
Rot Disease

Management of collar rot disease in chickpea by Trichoderma species

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Maximum Growth ◽  
Pot Experiment ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Collar Rot ◽  
Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

scholarly journals Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc

2016 ◽  
Vol 82 (2) ◽  
pp. 96-104 ◽  
Author(s):  
Simi Jacob ◽  
Ramgopal Rao Sajjalaguddam ◽  
K. Vijay Krishna Kumar ◽  
Rajeev Varshney ◽  
Hari Kishan Sudini
Keyword(s):  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Streptomyces Sp ◽  
Rot Disease

scholarly journals Comparison of Drip and Sprinkler Irrigation Systems for Applying Metam Sodium and Managing Stem Rot on Potato

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1211-1218 ◽  
Author(s):  
G. T. Browne ◽  
W. R. DeTar ◽  
B. L. Sanden ◽  
C. J. Phene
Keyword(s):  
Sclerotium Rolfsii ◽  
Sprinkler Irrigation ◽  
Stem Rot ◽  
Metam Sodium ◽  
Soil Infestation ◽  
Low Incidence ◽  
Rot Disease ◽  
Subsurface Drip ◽  
Soil Subsurface ◽  
Flat Ground

Drip and sprinkler systems were compared for effectiveness as preplant metam sodium chemigation systems and conduciveness to late-season development of stem rot disease on potato. Sclerotia of Sclerotium rolfsii were used in a bioassay to test efficacy of metam sodium treatments. Drip application of metam sodium (532 liters/ha, 32.8% a.i.) through lines at 7 cm of depth in preformed beds (depths from bed top unless stated otherwise) killed all test sclerotia at 15-, 30-, or 46-cm depths. Drip application of the metam sodium through drip lines at 41 or 46 cm of depth resulted in 0 to 17 or 68 to 80% survival, respectively, of test sclerotia at 15 cm of depth; but all the sclerotia at 30 or 46 cm of depth were killed. Compared with the drip applications, sprinkler chemigation with metam sodium generally treated beds less effectively (8 to 100% of sclerotia survived at 15 cm, 62 to 100% at 30 or 46 cm). On flat ground, drip and sprinkler chemigation (metam sodium, 560 liters/ha) performed equally (4, 37, and 77% survival at 15-, 45-, and 75-cm depths, respectively). After potato planting and artificial soil infestation with S. rolfsii (5 to 6 weeks before harvest), subsurface drip-irrigated plots (line depth of 41 or 46 cm) had lower incidence of stem rot disease at harvest (13 to 23% on tubers) than that in sprinkler plots (56 to 62%). The low incidence of disease was associated with relatively dry surface soil. Subsurface drip chemigation with metam sodium in preformed plant beds does not consistently eradicate S. rolfsii sclerotia near the upper bed surface but, in an arid climate, it is less conducive than sprinkler irrigation to development of stem rot disease of potato.

scholarly journals Sclerotium rolfsii, Penyebab Penyakit Busuk Pangkal Batang pada Hippeastrum sp.

2019 ◽  
Vol 15 (2) ◽  
pp. 53-58
Author(s):  
Antok Wahyu Sektiono ◽  
Syamsuddin Djauhari ◽  
Putri Devinta Pertiwi
Keyword(s):  
Host Range ◽  
Root Rot ◽  
Agar Medium ◽  
Sclerotium Rolfsii ◽  
Morphological Characteristics ◽  
Stem Rot ◽  
Potato Dextrose Agar Medium ◽  
White Mycelium ◽  
Rot Disease ◽  
Range Test

Sclerotium rolfsii, a the Causal Agent of Stem Rot Disease on Hippeastrum sp.Symptoms of stem rot that cause Hippeastrum sp. or red lily wither, leaves turn yellow, and eventually die found at Mangliawan Village, District of Pakis, Malang - East Java. The purpose of this study was to identify the pathogens that cause root rot disease on lily plants and find out their host range. Sclerotium from the symptomatic base of the plant was isolated on potato dextrose agar medium. Fungus was identified based on the morphological characteristics of the colonies and mycelium. Host range test of pathogen was carried out by manual inoculation on Rain lily (Zephyranthes) St. Bernard's lily (Chlorophytum) and Beach Spider lily (Hymenocallis). The results of the identification showed that the fungus had white mycelium and formed sclerotium. Sclerotium is irregularly rounded, white when young, and dark brown when ripe, and forms 10 days after incubation. In hyphae, there are branching, septa, and clam connections. Based on the morphological characteristics of the disease the fungus was identified as Sclerotium rolfsii. In the host range test, the fungus was able to infect rain lilies and paris lilies, but not in spider lilies. This is the first report of S. rolfsii infection in lily in Indonesia.

scholarly journals Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

2020 ◽  
pp. 25-31
Author(s):  
Mahbuba Kaniz Hasna ◽  
Md. Abul Kashem ◽  
Farid Ahmed
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Disease Incidence ◽  
Sclerotium Rolfsii ◽  
Antagonistic Activity ◽  
Root Weight ◽  
Dual Culture ◽  
Collar Rot ◽  
Rot Disease

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment. 

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