Structural organization of periodontium and morphological changes in its components in modeled periodontitis and its correction in rats

Introduction. The aim of our research was to study peculiar features of morphological changes in the components of the periodontium in the modeled acute periodontitis and its correction by stem cells. Materials and Methods. The results of histological study of periodontal tissues of the animals with acute experimental periodontitis, which was corrected using human mesenchymal stem cells as well as rat muscle stem cells, were analysed. Depending on the treatment, the animals were divided into 4 groups: the 1st group – the animals without pathology (intact); the 2nd – the animals with modeled acute periodontitis; the 3rd – the animals with periodontitis corrected by human mesenchymal stem cells, the 4th – the animals with periodontitis corrected using rat muscle stem cells. Results and Discussion. According to the results of the research it was established that correction by human mesenchymal stem cells and rats muscular stem cells had positive effect on a course of the modeled pathological process. The morphological changes characteristic of periodontitis gradually reduced, i.e. the normal blood supply to the tissues restored, the arteries were less full-blooded, the tone of their walls decreased. Consequently, the swelling of the tissues gradually decreased becoming of original size. The polymorphonuclear infiltration fields decreased and gradually disappeared. Osteoblasts promoted restoration of the bone structure of the jaw alveolar process. The size of the periodontal pocket decreased, and in some areas it fully closed as a result of complete adhesion of the mucous membrane of the gums to the tooth, which rose on its surface upwards. Conclusions. In the experimental modeling of acute periodontitis the destructive changes in the epithelium and connective tissue stroma, impaired blood flow, inflammatory response in the gum tissue were present. The correction by means of human mesenchymal stem cells as well as rat muscle stem cells had positive effect on the modeled pathological process: the morphological changes characteristic of periodontitis gradually reduced, normal blood supply to tissues restored, polymorphonuclear infiltration fields gradually decreased and disappeared. The bone structure of the jaw alveolar process restored owing to osteoblasts. The size of the periodontal pocket decreased; in some cases, they closed completely as a result of complete adhesion of the mucous membrane of the gums to the tooth, which rose on its surface upwards.

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Chronic senescent human mesenchymal stem cells as possible contributor to the wound healing disorder after exposure to the alkylating agent sulfur mustard

Archives of Toxicology ◽

10.1007/s00204-020-02946-5 ◽

2021 ◽

Vol 95 (2) ◽

pp. 727-747

Author(s):

Simone Rothmiller ◽

Niklas Jäger ◽

Nicole Meier ◽

Thimo Meyer ◽

Adrian Neu ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Alkylating Agent ◽

Chronic Wounds ◽

Sulfur Mustard ◽

Morphological Changes ◽

Human Mesenchymal Stem Cells ◽

Age Related

AbstractWound healing is a complex process, and disturbance of even a single mechanism can result in chronic ulcers developing after exposure to the alkylating agent sulfur mustard (SM). A possible contributor may be SM-induced chronic senescent mesenchymal stem cells (MSCs), unable to fulfil their regenerative role, by persisting over long time periods and creating a proinflammatory microenvironment. Here we show that senescence induction in human bone marrow derived MSCs was time- and concentration-dependent, and chronic senescence could be verified 3 weeks after exposure to between 10 and 40 µM SM. Morphological changes, reduced clonogenic and migration potential, longer scratch closure times, differences in senescence, motility and DNA damage response associated genes as well as increased levels of proinflammatory cytokines were revealed. Selective removal of these cells by senolytic drugs, in which ABT-263 showed initial potential in vitro, opens the possibility for an innovative treatment strategy for chronic wounds, but also tumors and age-related diseases.

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Stepwise morphological changes and cytoskeletal reorganization of human mesenchymal stem cells treated by short-time cyclic uniaxial stretch

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-017-0131-8 ◽

2017 ◽

Vol 53 (6) ◽

pp. 547-553 ◽

Cited By ~ 7

Author(s):

Azim Parandakh ◽

Mohammad Tafazzoli-Shadpour ◽

Mohammad-Mehdi Khani

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Morphological Changes ◽

Human Mesenchymal Stem Cells ◽

Cytoskeletal Reorganization ◽

Short Time

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Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

Nanomaterials ◽

10.3390/nano11030779 ◽

2021 ◽

Vol 11 (3) ◽

pp. 779

Author(s):

Chiara Argentati ◽

Francesco Morena ◽

Chiara Fontana ◽

Ilaria Tortorella ◽

Carla Emiliani ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Silica Nanoparticles ◽

Morphological Changes ◽

Mesoporous Silica Nanoparticles ◽

Human Mesenchymal Stem Cells ◽

Growth Curves ◽

Human Stem Cells

The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

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