Diversity of Fungal Trunk Pathogens Associated with Grapevine Dieback of Grapevine in Algeria

2014 ◽  
Vol 7 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Faiza Ammad ◽  
Messaoud Benchabane ◽  
Mohamed Toumi
Keyword(s):  
Grapevine Dieback

Identification, potential inoculum sources and pathogenicity of botryosphaeriaceous species associated with grapevine dieback disease in New Zealand

2011 ◽  
Vol 131 (3) ◽  
pp. 467-482 ◽  
Author(s):  
Nicholas T. Amponsah ◽  
E. Eirian Jones ◽  
Hayley J. Ridgway ◽  
Marlene V. Jaspers
Keyword(s):  
New Zealand ◽  
Inoculum Sources ◽  
Dieback Disease ◽  
Grapevine Dieback

scholarly journals First Report of Botryosphaeria dothidea, Diplodia seriata, and Neofusicoccum luteum Associated with Canker and Dieback of Grapevines in Tunisia

Plant Disease ◽  
2014 ◽  
Vol 98 (3) ◽  
pp. 420-420 ◽  
Author(s):  
S. Chebil ◽  
R. Fersi ◽  
A. Yakoub ◽  
S. Chenenaoui ◽  
M. Chattaoui ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Aerial Mycelium ◽  
Its2 Region ◽  
Pathogenicity Test ◽  
Botryosphaeria Dothidea ◽  
Diplodia Seriata ◽  
First Report ◽  
Grapevine Dieback ◽  
Pinus Spp ◽  
Neofusicoccum Luteum

In 2011, common symptoms of grapevine dieback were frequently observed in 2- to 5-year-old table grape (Vitis vinifera L.) cvs. in four vineyards located in northern Tunisia. The symptoms included dead spur and cordons, shoot dieback, and sunken necrotic bark lesions, which progressed into the trunk resulting in the death of large sections of the vine. Longitudinal and transversal sections of cordons and spurs from symptomatic vines revealed brown wedge-shaped cankers of hard consistency. Twelve symptomatic samples from spur and cordons were collected, surface disinfected by dipping into 5% (v/v) sodium hypochlorite for 2 min, and small pieces from the edge of necrotic and healthy tissue were removed and plated onto potato dextrose agar (PDA) at 25°C in the dark. Based on colony and conidia morphological characteristics, isolates were divided in three species, named Diplodia seriata, Botryosphaeria dothidea, and Neofusicoccum luteum. D. seriata colonies were gray-brown with dense aerial mycelium producing brown cylindric to ellipsoid conidia rounded at both ends and averaged 22.4 × 11.7 μm (n = 50). B. dothidea colonies were initially white with abundant aerial mycelium, gradually becoming dark green olivaceous. Conidia were fusiform to fusiform elliptical with a subobtuse apex and averaged 24.8 × 4.7 μm (n = 50). N. luteum colonies were initially pale to colorless, gradually darkening with age and becoming gray to dark gray producing a yellow pigment that diffuses into the agar. Conidia were hyaline, thin-walled, aseptate, fusiform to fusiform elliptical, and averaged 19.8 × 5.5 μm (n = 50). Identity of the different taxa was confirmed by sequence analyses of the internal transcribed spacer (ITS1-5.8S-ITS2) region of the rDNA and part of the elongation factor 1-alpha (EF1-α) gene. BLAST analysis of sequences indicated that six isolates were identified as D. seriata (GenBank: AY259094, AY343353), one isolate as B. dothidea (AY236949, AY786319) and one isolate as N. luteum (AY259091, AY573217). Sequences were deposited in GenBank under accessions from KC178817 to KC178824 and from KF546829 to KF546836 for ITS region and EF1-α gene, respectively. A pathogenicity test was conducted on detached green shoots cv. Italia for the eight Botryosphaeriaceae isolates. Shoots were inoculated by placing a colonized agar plug (5 mm diameter) from the margin of a 7-day-old colony on fresh wound sites made with a sterilized scalpel. Each wound was covered with moisturized cotton and sealed with Parafilm. Control shoots were inoculated using non-colonized PDA plugs. After 6 weeks, discoloration of xylem and phloem and necrosis with average length of 38.8, 17.6, and 11.2 mm were observed from inoculated shoots with D. seriata, N. luteum, and B. dothidea, respectively, and all three fungi were re-isolated from necrotic tissue, satisfying Koch's postulates. Control shoots showed no symptoms of the disease and no fungus was re-isolated. In Tunisia, Botryosphaeria-related dieback was reported only on citrus tree caused by B. ribis (2), on Pinus spp. caused by D. pinea (4), on Quercus spp. caused by D. corticola (3), and on olive tree (Olea europea) caused by D. seriata (1). To our knowledge, this is the first report of D. seriata, B. dothidea, and N. luteum associated with grapevine dieback in Tunisia. References: (1) M. Chattaoui et al. Plant Dis. 96:905, 2012. (2) H. S. Fawcett. Calif. Citrogr. 16:208, 1931. (3) B. T. Linaldeddu et al. J. Plant Pathol. 91:234. 2009. (4) B. T. Linaldeddu et al. Phytopathol. Mediterr. 47:258, 2008.

scholarly journals First report of Neofusicoccum batangarum causing grapevine dieback in Brazil

2020 ◽  
Vol 102 (3) ◽  
pp. 973-973
Author(s):  
Tamiris J. S. Rêgo ◽  
Fábio J. A. Silva ◽  
Kledson M. Santos ◽  
Marcos P. S. Câmara ◽  
Kamila C. Correia ◽  
...  
Keyword(s):  
First Report ◽  
Grapevine Dieback

scholarly journals Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia

Plant Disease ◽  
2018 ◽  
Vol 102 (8) ◽  
pp. 1489-1499 ◽  
Author(s):  
Regina Billones-Baaijens ◽  
José Ramón Úrbez-Torres ◽  
Meifang Liu ◽  
Matthew Ayres ◽  
Mark Sosnowski ◽  
...  
Keyword(s):  
Fungal Species ◽  
Molecular Tools ◽  
Spore Dispersal ◽  
Dispersal Patterns ◽  
Spore Trap ◽  
Synthetic Dna ◽  
Qpcr Method ◽  
Botryosphaeria Dieback ◽  
Grapevine Dieback ◽  
Rain Splash

Botryosphaeria dieback, caused by species of Botryosphaeriaceae, is an important grapevine trunk disease in Australia. Inocula produced by the pathogens are primarily dispersed by rain splash and wind and infect pruning wounds leading to cankers, dieback, and eventually death of vines. The objective of this study was to develop molecular tools to detect and quantify Botryosphaeriaceae inocula from the environment. These tools are essential for investigating spore dispersal patterns of Botryosphaeriaceae pathogens in Australian vineyards. DNA extraction protocols were evaluated and one modified protocol was found suitable for extracting Botryosphaeriaceae DNA from artificially and naturally inoculated Burkard volumetric spore sampler tapes. Multispecies primers and a hydrolysis probe for quantitative PCR (qPCR) were further developed to detect and quantify Botryosphaeriaceae inocula from environmental samples. Specificity tests showed that the multispecies primers were able to amplify the DNA of 10 Botryosphaeriaceae species (58 isolates) found in Australia while none of the 27 nontarget fungal species (90 isolates) tested were amplified. The qPCR assay was suitable for amplifying purified DNA, synthetic DNA fragments (gBlocks), and mixed DNA from spore trap tapes. The qPCR method developed in this study was shown to be rapid and sensitive in detecting Botryosphaeriaceae inocula from the environment using spore traps.

scholarly journals High Genetic Diversity and Species Complexity of Diaporthe Associated With Grapevine Dieback in China

2019 ◽  
Vol 10 ◽  
Author(s):  
Ishara S. Manawasinghe ◽  
Asha J. Dissanayake ◽  
Xinghong Li ◽  
Mei Liu ◽  
Dhanushka N. Wanasinghe ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
High Genetic Diversity ◽  
Grapevine Dieback

The potential importance of diversity in Eutypa lata and co-colonising fungi in explaining variation in development of grapevine dieback

1999 ◽  
Vol 103 (11) ◽  
pp. 1385-1390 ◽  
Author(s):  
J.-P. Péros ◽  
I. Jamaux-DesprÉAux ◽  
G. Berger ◽  
D. Gerba
Keyword(s):  
Potential Importance ◽  
Eutypa Lata ◽  
Grapevine Dieback

scholarly journals Pathogenicity of Botryosphaeriaceae Species Isolated from Grapevine Cankers in California

Plant Disease ◽  
2009 ◽  
Vol 93 (6) ◽  
pp. 584-592 ◽  
Author(s):  
J. R. Úrbez-Torres ◽  
W. D. Gubler
Keyword(s):  
Fungal Species ◽  
Botryosphaeria Dothidea ◽  
The Family ◽  
Vascular Discoloration ◽  
Grapevine Dieback ◽  
Weakly Virulent ◽  
Highly Virulent ◽  
Neofusicoccum Luteum ◽  
Botryosphaeriaceae Species

Fungal species in the family Botryosphaeriaceae have been recently recognized as the most common fungi isolated from grapevine (Vitis vinifera) cankers in California. However, the role of these fungi in causing grapevine dieback as well as their status as canker-causing agents was unknown. Therefore, pathogenicity studies were conducted to determine their importance as grapevine pathogens in California. A total of 72 isolates representing all nine Botryosphaeriaceae species isolated from grapevine cankers from California were used in five different pathogenicity studies. Overall, experiments showed all nine Botryosphaeriaceae species able to infect both young and mature tissues as well as green shoots of the new vegetative growth causing cankers, vascular discoloration, and/or otherwise dark streaking of the wood. However, virulence varied among species. Lasiodiplodia theobromae was the most virulent species followed by Neofusicoccum luteum, N. parvum, and N. australe, all categorized as highly virulent. Botryosphaeria dothidea was considered intermediately virulent and Diplodia mutila, D. seriata, Dothiorella iberica, and D. viticola were shown to be weakly virulent. This study shows species of Botryosphaeriaceae to be much more important pathogens on grapevines than originally thought, and some of them, in view of their virulence, should be considered high risk for causing severe and rapid canker and dieback diseases in the grapevine industry in California.

Species of Botryosphaeriaceae involved in grapevine dieback in China

2013 ◽  
Vol 61 (1) ◽  
pp. 221-236 ◽  
Author(s):  
Ji-Ye Yan ◽  
Yue Xie ◽  
Wei Zhang ◽  
Yong Wang ◽  
Jian-Kui Liu ◽  
...  
Keyword(s):  
Grapevine Dieback
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