Detection of New Delhi Metallo Beta Lactamase-1 (BlaNDM-1) Gene among a Group of Carbapenem Resistant Acinetobacter Baumannii Isolates

2016 ◽  
Vol 25 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Rania A. Hassan ◽  
Makram F. Attalah ◽  
Manal M. Darwish
Keyword(s):  
Acinetobacter Baumannii ◽  
New Delhi ◽  
Beta Lactamase ◽  
Carbapenem Resistant

scholarly journals Rapid Electrochemical Detection of New Delhi Metallo-beta-lactamase Genes To Enable Point-of-Care Testing of Carbapenem-Resistant Enterobacteriaceae

2015 ◽  
Vol 87 (15) ◽  
pp. 7738-7745 ◽  
Author(s):  
Jimmy Ming-Yuan Huang ◽  
Grace Henihan ◽  
Daniel Macdonald ◽  
Annette Michalowski ◽  
Kate Templeton ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Point Of Care ◽  
New Delhi ◽  
Beta Lactamase ◽  
Point Of Care Testing ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae

Identification of Carbapenem-Resistant Klebsiella pneumoniae with Emphasis on New Delhi Metallo-Beta-Lactamase-1 (blaNDM-1) in Bandar Abbas, South of Iran

2018 ◽  
Vol 24 (4) ◽  
pp. 447-454 ◽  
Author(s):  
Saeed Shoja ◽  
Maryam Ansari ◽  
Forogh Faridi ◽  
Mohsen Azad ◽  
Parivash Davoodian ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
New Delhi ◽  
Beta Lactamase ◽  
Carbapenem Resistant ◽  
Bandar Abbas

scholarly journals Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

2012 ◽  
Vol 6 (05) ◽  
pp. 457-461 ◽  
Author(s):  
Rima I El-Herte ◽  
George F Araj ◽  
Ghassan M Matar ◽  
Maysa Baroud ◽  
Zeina A Kanafani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
New Delhi ◽  
Beta Lactamase ◽  
The Novel ◽  
First Report ◽  
Carbapenem Resistant ◽  
Lactamase Gene ◽  
Carbapenem Resistant Enterobacteriaceae

Carbapenem resistance has been encountered globally with poor outcome of infected patients. NDM-1 (New Delhi metallo-beta-lactamase) gene containing organisms have emerged and are now spreading in all continents. This is the first report of Iraqi patients referred to Lebanon from whom carbapenem resistant Enterobacteriaceae were recovered. The genes involved in carbapenem resistance were bla-OXA-48   and the novel NDM-1. This report highlights the alarming introduction of such resistance among Enterobacteriaecae to this country.

A pentaplex real-time PCR assay for rapid identification of major beta-lactamase genes KPC, NDM, CTX, CMY, and OXA-48 directly from bacteria in blood

2021 ◽  
Vol 70 (12) ◽  
Author(s):  
Taalin R. Hoj ◽  
Bradley McNeely ◽  
Kylie Webber ◽  
Evelyn Welling ◽  
William G. Pitt ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Real Time ◽  
Real Time Pcr ◽  
Type Species ◽  
New Delhi ◽  
Beta Lactamase ◽  
Content Type ◽  
Link Type ◽  
Carbapenem Resistant

Introduction. Antibiotic resistance, particularly in cases of sepsis, has emerged as a growing global public health concern and economic burden. Current methods of blood culture and antimicrobial susceptibility testing of agents involved in sepsis can take as long as 3–5 days. It is vital to rapidly identify which antimicrobials can be used to effectively treat sepsis cases on an individual basis. Here, we present a pentaplex, real-time PCR-based assay that can quickly identify the most common beta-lactamase genes ( Klebsiella pneumoniae carbapenemase (KPC); New Delhi metallo-beta-lactamase (NDM); cefotaximase-Munich (CTX-M); cephamycin AmpC beta-lactamases (CMY); and Oxacillinase-48 (OXA-48)) from pathogens derived directly from the blood of patients presenting with bacterial septicemia. Aim. To develop an assay which can rapidly identify the most common beta-lactamase genes in Carbapenem-resistant Enterobacteriaceae bacteria (CREs) from the United States. Hypothesis/Gap Statement. Septicemia caused by carbapenem-resistant bacteria has a death rate of 40–60 %. Rapid diagnosis of antibiotic susceptibility directly from bacteria in blood by identification of beta-lactamase genes will greatly improve survival rates. In this work, we develop an assay capable of concurrently identifying the five most common beta-lactamase and carbapenemase genes. Methodology. Primers and probes were created which can identify all subtypes of Klebsiella pneumoniae carbapenemase (KPC); New Delhi metallo-beta-lactamase (NDM); cefotaximase-Munich (CTX); cephamycin AmpC beta-lactamase (CMY); and oxacillinase-48 (OXA-48). The assay was validated using 13 isolates containing various PCR targets from the Centre for Disease Control Antimicrobial Resistance Isolate Bank Enterobacterales Carbapenemase Diversity Panel. Blood obtained from volunteers was spiked with CREs and bacteria were separated, lysed, and subjected to analysis via the pentaplex assay. Results. This pentaplex assay successfully identified beta-lactamase genes derived from bacteria separated from blood at concentrations of 4–8 c.f.u. ml−1. Conclusion. This assay will improve patient outcomes by supplying physicians with critical drug resistance information within 2 h of septicemia onset, allowing them to prescribe effective antimicrobials corresponding to the resistance gene(s) present in the pathogen. In addition, information supplied by this assay will lessen the inappropriate use of broad-spectrum antimicrobials and prevent the evolution of further antibiotic resistance.

scholarly journals Increased Risk of Acquisition of New Delhi Metallo-Beta-Lactamase-Producing Carbapenem-Resistant Enterobacterales (NDM-CRE) among a Cohort of COVID-19 Patients in a Teaching Hospital in Tuscany, Italy

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 635 ◽  
Author(s):  
Andrea Davide Porretta ◽  
Angelo Baggiani ◽  
Guglielmo Arzilli ◽  
Virginia Casigliani ◽  
Tommaso Mariotti ◽  
...  
Keyword(s):  
Hospital Stay ◽  
Teaching Hospital ◽  
New Delhi ◽  
Beta Lactamase ◽  
Duration Of Hospital Stay ◽  
Carbapenem Resistant ◽  
Increased Risk

We describe the epidemiology of New Delhi Metallo-Beta-Lactamase-Producing Carbapenem-Resistant Enterobacterales (NDM-CRE) colonization/infection in a cohort of COVID-19 patients in an Italian teaching hospital. These patients had an increased risk of NDM-CRE acquisition versus the usual patients (75.9 vs. 25.3 cases/10,000 patient days). The co-infection significantly increased the duration of hospital stay (32.9 vs. 15.8 days).

scholarly journals Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae Strains Containing New Delhi Metallo-Beta-Lactamase Isolated from Two Patients in Vietnam: Table 1

2012 ◽  
Vol 51 (1) ◽  
pp. 373-374 ◽  
Author(s):  
Tran Huy Hoang ◽  
Heiman Wertheim ◽  
Nguyen Binh Minh ◽  
Tran Nhu Duong ◽  
Dang Duc Anh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
New Delhi ◽  
Beta Lactamase ◽  
Carbapenem Resistant

scholarly journals New Delhi Metallo-beta-lactamase around the world: An eReview using Google Maps

2014 ◽  
Vol 19 (20) ◽  
Author(s):  
M Berrazeg ◽  
S M Diene ◽  
L Medjahed ◽  
P Parola ◽  
M Drissi ◽  
...  
Keyword(s):  
Indian Subcontinent ◽  
Case Reports ◽  
Medical Community ◽  
Resistant Bacteria ◽  
New Delhi ◽  
Beta Lactamase ◽  
Google Maps ◽  
The United Kingdom ◽  
Carbapenem Resistant ◽  
Pubmed Database

Gram-negative carbapenem-resistant bacteria, in particular those producing New Delhi Metallo-beta-lactamase-1 (NDM-1), are a major global health problem. To inform the scientific and medical community in real time about worldwide dissemination of isolates of NDM-1-producing bacteria, we used the PubMed database to review all available publications from the first description in 2009 up to 31 December 2012, and created a regularly updated worldwide dissemination map using a web-based mapping application. We retrieved 33 reviews, and 136 case reports describing 950 isolates of NDM-1-producing bacteria. Klebsiella pneumoniae (n= 359) and Escherichia coli (n=268) were the most commonly reported bacteria producing NDM-1 enzyme. Several case reports of infections due to imported NDM-1 producing bacteria have been reported in a number of countries, including the United Kingdom, Italy, and Oman. In most cases (132/153, 86.3%), patients had connections with the Indian subcontinent or Balkan countries. Those infected were originally from these areas, had either spent time and/or been hospitalised there, or were potentially linked to other patients who had been hospitalised in these regions. By using Google Maps, we were able to trace spread of NDM-1-producing bacteria. We strongly encourage epidemiologists to use these types of interactive tools for surveillance purposes and use the information to prevent the spread and outbreaks of such bacteria.

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