Genetic Diversity for Yield and Its Components of 20 Bread Wheat Genotypes under Effect of Different Environments = الاختلافات الوراثية للمحصول ومكوناته لعشرين تركيب وراثي من قمح الخبز تحت تأثير بيئات مختلفة

2017 ◽  
Vol 21 (6) ◽  
pp. 1079-1098
Author(s):  
M. N. Abdelkader ◽  
I. S. M. Abdel-Latif
2016 ◽  
Vol 38 (3) ◽  
pp. 389-412
Author(s):  
Alhosein Hamada ◽  
Khalid Ibrahim

Author(s):  
Mohammad Bahman Sadeqi ◽  
Said Dadshani ◽  
Mohammad Yousefi ◽  
Gul Mohammad Ajir

Genetic diversity assessment is the principle component for conservation and characterization of germplasm. Genetic diversity study of Afghan bread wheat genotypes is a first step to identify and to select high performance genotypes and distribute to wheat breeding programs. The main objective of this study is to investigate of genetic diversity in 35 Afghan bread wheat genotypes by using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) markers. DNA extraction according to Cetyl Trimethyl Ammonium Bromide (CTAB) method was conducted and the total genomic DNA was isolated from each variety. Sixty-four SSR primer markers were used and eighteen EcoRI+(N)/MseI+(N) primer combinations with their primer sequences were used for selective polymerase chain reaction (PCR) amplification. Every SSR and AFLP fragment was scored as present (1) or absent (0) within all genotypes under study. Marker/ Value ratio of pairwise genetic distance between genotypes according to the SSRs data was from 0.508 to 0.691 with an average distance of 0.599. Relatively different grouping pattern in comparison to AFLP data observed through cluster analysis. Both types of molecular markers (AFLP and SSR) used in this research proved to be suitable for investigating genetic diversity in the genotypes of Afghan bread wheat, however, AFLP markers gave better view of genetically relationships among genotypes than the SSR markers. The grouping generated by AFLP data showed a special agreement with the origin regions of genotypes (Ariana-07 and Mazar-99 originating from the north of Afghanistan, Lalmi-03 and Kabul-02. Large number of DNA bands identified with AFLP markers might provide a better estimation of genetic similarity than those of SSR markers.


Euphytica ◽  
2005 ◽  
Vol 141 (1-2) ◽  
pp. 147-156 ◽  
Author(s):  
M. Esmaeilzadeh Moghaddam ◽  
R. M. Trethowan ◽  
H. M. William ◽  
A. Rezai ◽  
A. Arzani ◽  
...  

2017 ◽  
Vol 24 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Reza Amiri ◽  
Shahryar Sasani ◽  
Saeid Jalali-Honarmand ◽  
Ali Rasaei ◽  
Behnaz Seifolahpour ◽  
...  

2014 ◽  
Vol 24 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Sutapa Sarkar ◽  
Anju M. Singh ◽  
Arvind K. Ahlawat ◽  
Mridul Chakraborti ◽  
Santosh K. Singh

2013 ◽  
Vol 59 (3) ◽  
pp. 101-110
Author(s):  
Martina Hudcovicová ◽  
Katarína Ondreičková ◽  
Pavol Hauptvogel ◽  
Ján Kraic

Abstract A set of 33 wheat EST-SSR markers was designed and 18 from them were polymorphic and used for assessment of genetic diversity within 36 introduced genotypes of hexaploid bread wheat. Altogether 105 alleles were detected, in average 3.18 alleles per locus. Maximum number of alleles 14 was detected at the locus TDI389708. Five the most polymorphic markers were used for the evaluation and comparison of genetic variation within 46 domestic (Slovak) wheat genotypes and 36 introduced (foreign) wheat genotypes. The number of alleles per used primer pair within domestic genotypes varied from 7 to 19, with an average of 13.2 alleles, an average gene diversity 0.846 and PIC 0.980 per locus. The number of alleles per primer within introduced genotypes varied from 7 to 14, with an average of 10.8 alleles, an average gene diversity 0.780 and PIC 0.958 per locus. The level of polymorphism in EST- SSRs was sufficient for discrimination between genotypes and variation within domestic genotypes was slightly higher than in introduced genotypes. Variation revealed by 5 selected EST-SSR markers clustered genotypes according to origin. Domestic and introduced wheats were grouped distinctly into two separate groups.


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