Assessment of Genetic Diversity in Durum and Bread Wheat Genotypes Based on Drought Tolerance and SSR Markers

2021 ◽  
Vol 9 (2) ◽  
pp. 89-103
Author(s):  
Mahmoud A. El-Rawy ◽  
Mohamed I. Hassan
Keyword(s):  
Genetic Diversity ◽  
Drought Tolerance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Wheat Genotypes

scholarly journals Investigation of Genetic Diversity in Afghan Bread Wheat Genotypes Using SSR and AFLP Markers

2019 ◽  
Vol 7 (9) ◽  
pp. 1263
Author(s):  
Mohammad Bahman Sadeqi ◽  
Said Dadshani ◽  
Mohammad Yousefi ◽  
Gul Mohammad Ajir
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Average Distance ◽  
Wheat Breeding ◽  
Aflp Markers ◽  
Ammonium Bromide ◽  
Aflp Data ◽  
Wheat Genotypes ◽  
Diversity Assessment

Genetic diversity assessment is the principle component for conservation and characterization of germplasm. Genetic diversity study of Afghan bread wheat genotypes is a first step to identify and to select high performance genotypes and distribute to wheat breeding programs. The main objective of this study is to investigate of genetic diversity in 35 Afghan bread wheat genotypes by using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) markers. DNA extraction according to Cetyl Trimethyl Ammonium Bromide (CTAB) method was conducted and the total genomic DNA was isolated from each variety. Sixty-four SSR primer markers were used and eighteen EcoRI+(N)/MseI+(N) primer combinations with their primer sequences were used for selective polymerase chain reaction (PCR) amplification. Every SSR and AFLP fragment was scored as present (1) or absent (0) within all genotypes under study. Marker/ Value ratio of pairwise genetic distance between genotypes according to the SSRs data was from 0.508 to 0.691 with an average distance of 0.599. Relatively different grouping pattern in comparison to AFLP data observed through cluster analysis. Both types of molecular markers (AFLP and SSR) used in this research proved to be suitable for investigating genetic diversity in the genotypes of Afghan bread wheat, however, AFLP markers gave better view of genetically relationships among genotypes than the SSR markers. The grouping generated by AFLP data showed a special agreement with the origin regions of genotypes (Ariana-07 and Mazar-99 originating from the north of Afghanistan, Lalmi-03 and Kabul-02. Large number of DNA bands identified with AFLP markers might provide a better estimation of genetic similarity than those of SSR markers.

scholarly journals Genetic Diversity in Domestic and Introduced Wheats

2013 ◽  
Vol 59 (3) ◽  
pp. 101-110
Author(s):  
Martina Hudcovicová ◽  
Katarína Ondreičková ◽  
Pavol Hauptvogel ◽  
Ján Kraic
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Polymorphic Markers ◽  
Wheat Genotypes ◽  
Average Gene Diversity ◽  
Average Gene

Abstract A set of 33 wheat EST-SSR markers was designed and 18 from them were polymorphic and used for assessment of genetic diversity within 36 introduced genotypes of hexaploid bread wheat. Altogether 105 alleles were detected, in average 3.18 alleles per locus. Maximum number of alleles 14 was detected at the locus TDI389708. Five the most polymorphic markers were used for the evaluation and comparison of genetic variation within 46 domestic (Slovak) wheat genotypes and 36 introduced (foreign) wheat genotypes. The number of alleles per used primer pair within domestic genotypes varied from 7 to 19, with an average of 13.2 alleles, an average gene diversity 0.846 and PIC 0.980 per locus. The number of alleles per primer within introduced genotypes varied from 7 to 14, with an average of 10.8 alleles, an average gene diversity 0.780 and PIC 0.958 per locus. The level of polymorphism in EST- SSRs was sufficient for discrimination between genotypes and variation within domestic genotypes was slightly higher than in introduced genotypes. Variation revealed by 5 selected EST-SSR markers clustered genotypes according to origin. Domestic and introduced wheats were grouped distinctly into two separate groups.

scholarly journals Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mahmoud M. Gaballah ◽  
Azza M. Metwally ◽  
Milan Skalicky ◽  
Mohamed M. Hassan ◽  
Marian Brestic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Ssr Markers ◽  
Similarity Index ◽  
Stress Conditions ◽  
Yield Potential ◽  
Drought Tolerant ◽  
Rice Genotypes

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

Evaluation of genetic diversity among bread wheat varieties and landraces of Pakistan by SSR markers

2009 ◽  
Vol 37 (4) ◽  
pp. 489-498 ◽  
Author(s):  
N. Iqbal ◽  
A. Tabasum ◽  
H. Sayed ◽  
A. Hameed
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Wheat Varieties

Screening for drought tolerance in wheat genotypes by morphological and SSR markers

2020 ◽  
Vol 24 (1) ◽  
pp. 27-39
Author(s):  
Muhammad Shahidul Haque ◽  
Nihar Ranjan Saha ◽  
Muhammad Tariqul Islam ◽  
Muhammad Monirul Islam ◽  
Soo-Jeong Kwon ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Ssr Markers ◽  
Wheat Genotypes

Drought tolerance of some bread wheat genotypes in Ethiopia

2001 ◽  
Vol 9 (2) ◽  
Author(s):  
Bedada Girma ◽  
Zewdie Alemayehu ◽  
Solomon Gelalcha
Keyword(s):  
Drought Tolerance ◽  
Bread Wheat ◽  
Wheat Genotypes
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