Diagnostic utility of measuring serum amyloid A with a latex agglutination turbidimetric immunoassay in bovine mastitis: comparison with haptoglobin and alpha 1 acid glycoprotein

Serum amyloid A protein (SAA) is a sensitive acute phase react ant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzymeimmunoassay showed good agreement ( r = 0·988). The normal range was 0·17–10·0 mg/L [mean (SD)]. This rapid method should prove useful in clinical laboratories.

Download Full-text

Protective effects of α1-acid glycoprotein and serum amyloid A on concanavalin A-induced liver failure via interleukin-6 induction by ME3738

European Journal of Pharmacology ◽

10.1016/j.ejphar.2006.05.003 ◽

2006 ◽

Vol 541 (3) ◽

pp. 205-210 ◽

Cited By ~ 10

Author(s):

Hiroyuki Kuzuhara ◽

Yoshihisa Nakano ◽

Nobuyuki Yamashita ◽

Masako Imai ◽

Yuji Kawamura ◽

...

Keyword(s):

Liver Failure ◽

Concanavalin A ◽

Interleukin 6 ◽

Serum Amyloid A ◽

Serum Amyloid ◽

Protective Effects ◽

Acid Glycoprotein ◽

Amyloid A

Download Full-text

Changes in concentrations of serum amyloid A protein, α1-acid glycoprotein, haptoglobin, and C-reactive protein in feline sera due to induced inflammation and surgery

Veterinary Immunology and Immunopathology ◽

10.1016/s0165-2427(99)00012-4 ◽

1999 ◽

Vol 68 (1) ◽

pp. 91-98 ◽

Cited By ~ 64

Author(s):

Taketsugu Kajikawa ◽

Akihito Furuta ◽

Takafumi Onishi ◽

Tomoko Tajima ◽

Shunji Sugii

Keyword(s):

Serum Amyloid A ◽

Serum Amyloid ◽

C Reactive Protein ◽

Acid Glycoprotein ◽

Reactive Protein ◽

Amyloid A ◽

Serum Amyloid A Protein

Download Full-text

Acute-phase protein concentrations in serum of clinically healthy and diseased European bison (Bison bonasus) – preliminary study

BMC Veterinary Research ◽

10.1186/s12917-021-03133-z ◽

2022 ◽

Vol 18 (1) ◽

Author(s):

Małgorzata Pomorska-Mól ◽

Kacper Libera ◽

Magdalena Larska ◽

Michał K. Krzysiak

Keyword(s):

Health Status ◽

Serum Concentration ◽

Acute Phase ◽

Serum Amyloid A ◽

Health Condition ◽

Serum Amyloid ◽

European Bison ◽

Acid Glycoprotein ◽

Amyloid A ◽

The Poor

Abstract Background This is the first report describing levels of APPs in European bison. Serum concentration of acute phase proteins (APPs) may be helpful to assess general health status in wildlife and potentially useful in selecting animals for elimination. Since there is a lack of literature data regarding concentration of APPs in European bisons, establishment of the reference values is also needed. Methods A total of 87 European bison from Polish populations were divided into two groups: (1) healthy: immobilized for transportation, placing a telemetry collar and routine diagnostic purposes; and (2) selectively culled due to the poor health condition. The serum concentration of haptoglobin, serum amyloid A and α1-acid-glycoprotein were determined using commercial quantitative ELISA assays. Since none of the variables met the normality assumptions, non-parametric Mann-Whitney U test was used for all comparisons. Statistical significance was set at p < 0.05. Statistical analyses were performed using Statistica 13.3 (Tibco, USA). Results The concentration of haptoglobin and serum amyloid A was significantly higher in animals culled (euthanised) due to the poor condition in respect to the clinically healthy European bison. The levels of α1-acid-glycoprotein did not show statistical difference between healthy and sick animals. Conclusions Correlation between APPs concertation and health status was proven, therefore the determination of selected APPs may be considered in future as auxiliary predictive tool in assessing European bison health condition.

Download Full-text