scholarly journals Preliminary study of the gene expression of sulfation and degradation enzymes for chondroitin sulfate in glycerol-treated C2C12 myoblast cells

2022 ◽  
Author(s):  
Yoshinao Z. HOSAKA ◽  
Sota WASHIE ◽  
Katsuhiko WARITA
Keyword(s):  
Gene Expression ◽  
Chondroitin Sulfate ◽  
C2c12 Myoblast ◽  
Preliminary Study ◽  
Myoblast Cells

110 EFFECT OF MYOSTATIN ON GENE EXPRESSION OF GLUTAMINE SYNTHASE IN C2C12 MYOBLAST CELLS.

2004 ◽  
Vol 52 (Suppl 1) ◽  
pp. S98.1-S98
Author(s):  
W. E. Taylor ◽  
I. Harnden ◽  
B. Salehian
Keyword(s):  
Gene Expression ◽  
C2c12 Myoblast ◽  
Glutamine Synthase ◽  
Myoblast Cells

scholarly journals A Study of C2C12 Myoblast Bioenergetics in Response to the CCG-1423 Rho A Inhibitor

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. 689-689
Author(s):  
Bachkhoa Nguyen ◽  
Fathima Ameer ◽  
Jasmine Crane ◽  
Gohar Azhar ◽  
Xiaomin Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oxygen Consumption ◽  
Mitochondrial Function ◽  
Consumption Rate ◽  
C2c12 Cells ◽  
C2c12 Myoblast ◽  
Dependent Manner ◽  
Mitochondrial Oxygen Consumption ◽  
Myoblast Cells ◽  
Dose Dependent

Abstract CCG-1423 is a Rho A pathway inhibitor which has been reported to inhibit Rho/SRF-mediated transcriptional regulation. SRF and SRF cofactors, which include ternary complex factors (TCFs) and myocardin-related transcription factor (MRTF), regulate various cellular functions. The Rho/SRF signaling pathway also regulates the sirtuin 2 (SIRT2) gene that contains a classic serum response element (SRE) sequence. Current research on CCG-1423 focuses on gene expression levels of SRF in response to CCG-1423 and how SRF levels affect the cells; the studies are focused on cell morphology, migration, viability/reproduction, and overall function. The pathways of this inhibitor have yet to be fully elucidated, but several have been suggested with good evidence. Our goal is to study the effect of CCG-1423 on mitochondrial function and gene expression of cells. In this work C2C12 myoblast cells have been used as an in-vitro model to study cellular bioenergetics and variations in gene expressions induced by CCG-1423. The effect of CCG-1423 on mitochondrial function was determined by measuring the mitochondrial oxygen consumption rate and glycolysis rate after treating C2C12 cells with varying concentrations of CCG-1423 overnight. In C2C12 myoblast cells, CCG-1423 treatment significantly reduced mitochondrial oxygen consumption rate (OCR) in a dose-dependent manner. However, treatment of C2C12 cells with CCG-1423 overnight increased the extracellular acidification rate (ECAR) in a dose-dependent manner. By indicating that CCG-1423 represses mitochondrial respiration via the Rho-SRF signaling pathway, the results of this study may enable a better understanding of the bioenergetics of the cell in the aging body.

Root Cementum May Modulate Gene Expression During Periodontal Regeneration: A Preliminary Study in Humans

2008 ◽  
Vol 79 (2) ◽  
pp. 323-331 ◽  
Author(s):  
Patricia F. Gonçalves ◽  
Liana L. Lima ◽  
Enilson A. Sallum ◽  
Márcio Z. Casati ◽  
Francisco H. Nociti
Keyword(s):  
Gene Expression ◽  
Periodontal Regeneration ◽  
Preliminary Study

Gene expression profiling of ovine keratinocytes stimulated withPsoroptes ovismite antigen - a preliminary study

2009 ◽  
Vol 31 (6) ◽  
pp. 304-311 ◽  
Author(s):  
C. A. WATKINS ◽  
A. MACKELLAR ◽  
D. FREW ◽  
C. MACKIE ◽  
A. GEORGE ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Preliminary Study ◽  
Antigen A

scholarly journals Altered Gene Expression in Acne Vulgaris Patients Treated by Oral Isotretinoin: A Preliminary Study

2020 ◽  
Vol Volume 13 ◽  
pp. 385-395
Author(s):  
Yuchen Jiang ◽  
Haiyan Chen ◽  
Le Han ◽  
Xiaoyuan Xie ◽  
Yue Zheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acne Vulgaris ◽  
Altered Gene Expression ◽  
Oral Isotretinoin ◽  
Preliminary Study ◽  
Altered Gene
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