scholarly journals Adhesion Activity of Campylobacter jejuni for Intestinal Epithelial Cells and Mucus and Erythrocytes.

1994 ◽  
Vol 56 (6) ◽  
pp. 1123-1127 ◽  
Author(s):  
Soichi MARUYAMA ◽  
Yasuji KATSUBE
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Adhesion Activity

scholarly journals A σ28-Regulated Nonflagella Gene Contributes to Virulence of Campylobacter jejuni 81-176

2006 ◽  
Vol 74 (1) ◽  
pp. 769-772 ◽  
Author(s):  
Scarlett Goon ◽  
Cheryl P. Ewing ◽  
Maria Lorenzo ◽  
Dawn Pattarini ◽  
Gary Majam ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Diarrheal Disease ◽  
Intestinal Epithelial Cells ◽  
Disease Model ◽  
Intestinal Epithelial ◽  
Model Expression

ABSTRACT A Campylobacter jejuni 81-176 mutant in Cj0977 was fully motile but reduced >3 logs compared to the parent in invasion of intestinal epithelial cells in vitro. The mutant was also attenuated in a ferret diarrheal disease model. Expression of Cj0977 protein was dependent on a minimal flagella structure.

scholarly journals CapC, a Novel Autotransporter and Virulence Factor ofCampylobacter jejuni

2018 ◽  
Vol 84 (16) ◽  
Author(s):  
Jai W. Mehat ◽  
Simon F. Park ◽  
Arnoud H. M. van Vliet ◽  
Roberto M. La Ragione
Keyword(s):  
Public Health ◽  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Virulence Factor ◽  
Causative Agent ◽  
Galleria Mellonella ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Content Type ◽  
Reduced Adherence

ABSTRACTCampylobacter jejuniis recognized as an important causative agent of bacterial gastroenteritis in the developed world. Despite the identification of several factors contributing to infection, characterization of the virulence strategies employed byC. jejuniremains a significant challenge. Bacterial autotransporter proteins are a major class of secretory proteins in Gram-negative bacteria, and notably, many autotransporter proteins contribute to bacterial virulence. The aim of this study was to characterize theC. jejuni81116 C8J_1278 gene (capC), predicted to encode an autotransporter protein, and examine the contribution of this factor to virulence ofC. jejuni. The predicted CapC protein has a number of features that are consistent with autotransporters, including the N-terminal signal sequence and the C-terminal β-barrel domain and was determined to localize to the outer membrane. Inactivation of thecapCgene inC. jejuni81116 andC. jejuniM1 resulted in reduced insecticidal activity inGalleria mellonellalarvae. Furthermore,C. jejuni capCmutants displayed significantly reduced adherence to and invasion of nonpolarized, partially differentiated Caco-2 and T84 intestinal epithelial cells. Gentamicin treatment showed that the reduced invasion of thecapCmutant is primarily caused by reduced adherence to intestinal epithelial cells, not by reduced invasion capability.C. jejuni capCmutants caused reduced interleukin 8 (IL-8) secretion from intestinal epithelial cells and elicited a significantly diminished immune reaction inGallerialarvae, indicating that CapC functions as an immunogen. In conclusion, CapC is a new virulence determinant ofC. jejunithat contributes to the integral infection process of adhesion to human intestinal epithelial cells.IMPORTANCECampylobacter jejuniis a major causative agent of human gastroenteritis, making this zoonotic pathogen of significant importance to human and veterinary public health worldwide. The mechanisms by whichC. jejuniinteracts with intestinal epithelial cells and causes disease are still poorly understood due, in part, to the heterogeneity ofC. jejuniinfection biology. Given the importance ofC. jejunito public health, the need to characterize novel and existing virulence mechanisms is apparent. The significance of our research is in demonstrating the role of CapC, a novel virulence factor inC. jejunithat contributes to adhesion and invasion of the intestinal epithelium, thereby in part, addressing the dearth of knowledge concerning the factors involved inCampylobacterpathogenesis and the variation observed in the severity of human infection.

scholarly journals Polyphosphate kinases modulate Campylobacter jejuni outer membrane constituents and alter its capacity to invade and survive in intestinal epithelial cells in vitro

2015 ◽  
Vol 4 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Ruby Pina-Mimbela ◽  
Jesús Arcos Madrid ◽  
Anand Kumar ◽  
Jordi B Torrelles ◽  
Gireesh Rajashekara
Keyword(s):  
Epithelial Cells ◽  
Outer Membrane ◽  
Campylobacter Jejuni ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

An adapted in vitro assay to assess Campylobacter jejuni interaction with intestinal epithelial cells: Taking into stimulation with TNFα

2018 ◽  
Vol 149 ◽  
pp. 67-72 ◽  
Author(s):  
Ramila Cristiane Rodrigues ◽  
Anne-Lise Pocheron ◽  
Jean-Michel Cappelier ◽  
Odile Tresse ◽  
Nabila Haddad
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Intestinal Epithelial Cells ◽  
In Vitro Assay ◽  
Intestinal Epithelial ◽  
Vitro Assay

scholarly journals A Deep-Rough Mutant of Campylobacter jejuni 81-176 Is Noninvasive for Intestinal Epithelial Cells

2004 ◽  
Vol 72 (4) ◽  
pp. 2452-2455 ◽  
Author(s):  
Margaret I. Kanipes ◽  
Lindsay C. Holder ◽  
Adrian T. Corcoran ◽  
Anthony P. Moran ◽  
Patricia Guerry
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Type Strain ◽  
Wild Type Strain ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Wild Type ◽  
Increased Sensitivity ◽  
Rough Mutant

ABSTRACT A waaF mutant of Campylobacter jejuni 81-176 showed decreased invasion of INT407 cells in vitro and increased sensitivity to some antibiotics compared to what was seen with the wild-type strain.

Adherence to and Invasion of Human Intestinal Epithelial Cells by Campylobacter jejuni and Campylobacter coli Isolates from Retail Meat Products

2006 ◽  
Vol 69 (4) ◽  
pp. 768-774 ◽  
Author(s):  
JIE ZHENG ◽  
JIANGHONG MENG ◽  
SHAOHUA ZHAO ◽  
RUBY SINGH ◽  
WENXIA SONG
Keyword(s):  
Epithelial Cells ◽  
Campylobacter Jejuni ◽  
Virulence Genes ◽  
Intestinal Epithelial Cells ◽  
Clinical Strain ◽  
Campylobacter Coli ◽  
Intestinal Epithelial ◽  
T84 Cells ◽  
Human Intestinal Epithelial Cells ◽  
Retail Meat

The abilities of 34 Campylobacter jejuni and 9 Campylobacter coli isolates recovered from retail meats to adhere to and invade human intestinal epithelial T84 cells were examined and compared with those of a well-characterized human clinical strain, C. jejuni 81-176, to better assess the pathogenic potential of these meat isolates. The meat isolates exhibited a wide range of adherence and invasion abilities; a few of the isolates adhered to and invaded T84 cells almost as well as did C. jejuni 81-176. There was a significant correlation between the adherence ability and the invasion ability of the Campylobacter isolates. The presence of eight putative virulence genes in these Campylobacter isolates that are potentially responsible for adherence and invasion or that encode cytolethal distending toxin was determined using PCR. All Campylobacter isolates possessed flaA, cadF, pldA, cdtA, cdtB, and cdtC, and most (91%) also contained the ciaB gene. However, the virB11 gene, carried by virulence plasmid pVir, was absent in almost all the Campylobacter isolates. Our findings indicated that C. jejuni and C. coli present in retail meat were diverse in their ability to adhere to and invade human intestinal epithelial cells and that the putative virulence genes were widespread among the Campylobacter isolates. Thus, despite of the presence of the putative virulence genes, only some but not all Campylobacter strains isolated from retail meat can effectively invade human intestinal epithelial cells in vitro.

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