scholarly journals Evaluation of Equine Infectious Anemia Virus Core Proteins Produced in a Baculovirus Expression System in Agar Gel Immunodiffusion Test and Enzyme-Linked Immunosorbent Assay.

1998 ◽  
Vol 60 (12) ◽  
pp. 1361-1362 ◽  
Author(s):  
Xian-Gang KONG ◽  
Hai PANG ◽  
Takeo SUGIURA ◽  
Yoshitsugu MATSUMOTO ◽  
Takashi ONODERA ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agar Gel ◽  
Virus Core ◽  
Baculovirus Expression ◽  
Core Proteins ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Comparison of Diagnostic Tests for the Detection of Equine Infectious Anemia Antibody

1989 ◽  
Vol 1 (1) ◽  
pp. 50-52 ◽  
Author(s):  
Tatsuo Matsushita ◽  
Lyndal K. Hesterberg ◽  
James P. Porter ◽  
Barbara J. Smith ◽  
Louis E. Newman
Keyword(s):  
Western Blot ◽  
Diagnostic Tests ◽  
Equine Infectious Anemia Virus ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Veterinary Services ◽  
Agar Gel ◽  
Equine Infectious Anemia ◽  
Low Levels ◽  
Anemia Virus

Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.

scholarly journals Western Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies

2007 ◽  
Vol 14 (12) ◽  
pp. 1646-1648 ◽  
Author(s):  
I. Alvarez ◽  
G. Gutierrez ◽  
E. Ostlund ◽  
M. Barrandeguy ◽  
K. Trono
Keyword(s):  
Western Blot ◽  
Capsid Protein ◽  
Diagnostic Tool ◽  
Equine Infectious Anemia Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agar Gel ◽  
Western Blot Assay ◽  
Specific Antibodies ◽  
Equine Infectious Anemia ◽  
Anemia Virus

ABSTRACT We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.

scholarly journals SEROLOGICAL DIAGNOSIS OF EQUINE INFECTIOUS ANEMIA VÍRUS INFECTION IN THE CENTRAL REGION OF THE RIO GRANDE DO SUL STATE

1992 ◽  
Vol 22 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Marlon Cezar Rebelatto ◽  
Clóvis de Oliveira ◽  
Rudi Weiblen ◽  
Saul Fontoura da Silva ◽  
Luiz Sérgio Segala de Oliveira
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Serological Diagnosis ◽  
Rio Grande Do Sul ◽  
Serum Samples ◽  
Agar Gel ◽  
Blood Sucking ◽  
Equine Infectious Anemia ◽  
South Of Brazil ◽  
Anemia Virus ◽  
Santa Maria

This paper consists of a brief review on equine Infectious anemia added of the results of serological diagnosis of this infection performed at the Federal University of Santa Maria, RS, Brazil, between 1979 to 1990. A total of 7,035 serum samples were tested by the agar gel immunodiffusion test (Coggins test), to which 40 (0,57%) reacted positively. This percentage of positivity is lower than in other regions of Brazil, probably due to the lack of predisposing factors for equine infectious anemia virus spread, such as, a low density of blood-sucking insects in the South of Brazil and the seldom use of massive therapies and vaccinations in the region.

scholarly journals Identification and genetic characterization of equine infectious anemia virus in Western Balkans

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Diana Lupulovic ◽  
Sara Savić ◽  
Delphine Gaudaire ◽  
Nicolas Berthet ◽  
Živoslav Grgić ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Clinical Signs ◽  
Full Genome Sequence ◽  
Enzyme Linked Immunosorbent Assay ◽  
Full Genome ◽  
Western Balkans ◽  
Full Genome Sequencing ◽  
Horse Population ◽  
Equine Infectious Anemia ◽  
Anemia Virus

Abstract Background Equine infectious anemia (EIA) is a viral disease, caused by the Equine Infectious Anemia virus (EIAV) belonging to the Retroviridae family, genus Lentivirus. Horses (or equids) infected with EIAV are lifelong carriers and they remain contagious for other horses even in the absence of clinical signs. So far, EIAV infection has been reported among horses in North and South America, France, Germany, Italy, Hungary and Romania, with no publication regarding the presence of EIAV in horses in Serbia. To determine the circulation of EIAV among, approximately, the 5000 horses of the Vojvodina region, northern part of Serbia, 316 serum undergone serological testing for EIA. Then, identification and full genome sequencing using next generation sequencing was performed from one EIA positive horse. Results the 316 sera were tested with 3 different commercial agar gel immunodiffusion (AGID) tests and two different commercial enzyme-linked immunosorbent assay (ELISA). With the three AGID kits, 311 (98.4%) among the 316 tested sera were negative and only five (1.6%) sera were positive for EIA. Some discrepancies were seen for the two ELISA kits tested since one exhibited the same results as AGID test and the second gave 295 sera with negative results, five with a positive result and 16 with doubtful outcome. Phylogenetic analysis performed using the full genome sequence showed that EIAV characterized from a horse in Serbia is different from those identify so fare around the world and form a distinct and separate group together with another EIAV strain. Conclusions This study demonstrate for the first time that EIAV is circulating at a low level in the horse population from the Northern part of Serbia. Interestingly, phylogenetic data indicates that this EIAV from the western Balkan region of Europe belongs to a new cluster.

scholarly journals Molecular Cloning, Expression, and Antigenicity of Seto Virus Belonging to Genogroup I Norwalk-Like Viruses

2000 ◽  
Vol 38 (9) ◽  
pp. 3492-3494 ◽  
Author(s):  
Shinichi Kobayashi ◽  
Kenji Sakae ◽  
Yasumoto Suzuki ◽  
Kuniko Shinozaki ◽  
Mineyuki Okada ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Capsid Protein ◽  
Immunosorbent Assay ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid ◽  
Specific Detection ◽  
Japanese Strain ◽  
Baculovirus Expression

The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.

scholarly journals Serological Method Using Recombinant S2 Protein To Differentiate Equine Infectious Anemia Virus (EIAV)-Infected and EIAV-Vaccinated Horses

2004 ◽  
Vol 11 (6) ◽  
pp. 1120-1129 ◽  
Author(s):  
Sha Jin ◽  
Charles J. Issel ◽  
Ronald C. Montelaro
Keyword(s):  
Vaccine Strain ◽  
Equine Infectious Anemia Virus ◽  
Genetically Engineered ◽  
Commercial Application ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Antibodies ◽  
Serological Method ◽  
Live Virus ◽  
Equine Infectious Anemia ◽  
Anemia Virus

ABSTRACT We recently reported a highly protective attenuated live virus vaccine for equine infectious anemia virus (EIAV) based on a proviral construct (EIAVUKΔS2) with a genetically engineered mutation in the viral S2 gene that eliminates expression of this accessory protein. While the EIAVUKΔS2 vaccine provides protection from detectable infection by experimental challenge with highly virulent virus, the potential for commercial application of this vaccine is complicated by the fact that horses inoculated with the EIAVUKΔS2 vaccine strain become seropositive in various reference diagnostic assays based on detection of antibodies to virion core or envelope proteins. To address this issue, we describe here the development and optimization of a new serologic EIAV diagnostic enzyme-linked immunosorbent assay (ELISA) to detect serum antibodies to the EIAV S2 protein that are produced in infected horses but not in horses inoculated with the EIAVUKΔS2 vaccine virus. The test S2 protein antigen was developed using the S2 gene sequence from the EIAVUK strain of virus and a series of modifications to facilitate production and purification of the diagnostic antigen, designated HS2G. Using this HS2G as antigen, we describe the development of an affinity ELISA that provides a sensitive and specific detection of S2-specific serum antibodies in experimentally and field-infected horses (22 of 24), without detectable reactivity with immune serum from uninfected (12 of 12) or vaccinated (29 of 29) horses. These data indicate that the S2-based diagnostic ELISA has the potential to accurately differentiate horses infected with EIAV from horses inoculated with an attenuated EIAV vaccine strain with a mutant S2 gene.

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