scholarly journals SEROLOGICAL DIAGNOSIS OF EQUINE INFECTIOUS ANEMIA VÍRUS INFECTION IN THE CENTRAL REGION OF THE RIO GRANDE DO SUL STATE

1992 ◽  
Vol 22 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Marlon Cezar Rebelatto ◽  
Clóvis de Oliveira ◽  
Rudi Weiblen ◽  
Saul Fontoura da Silva ◽  
Luiz Sérgio Segala de Oliveira
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Serological Diagnosis ◽  
Rio Grande Do Sul ◽  
Serum Samples ◽  
Agar Gel ◽  
Blood Sucking ◽  
Equine Infectious Anemia ◽  
South Of Brazil ◽  
Anemia Virus ◽  
Santa Maria

This paper consists of a brief review on equine Infectious anemia added of the results of serological diagnosis of this infection performed at the Federal University of Santa Maria, RS, Brazil, between 1979 to 1990. A total of 7,035 serum samples were tested by the agar gel immunodiffusion test (Coggins test), to which 40 (0,57%) reacted positively. This percentage of positivity is lower than in other regions of Brazil, probably due to the lack of predisposing factors for equine infectious anemia virus spread, such as, a low density of blood-sucking insects in the South of Brazil and the seldom use of massive therapies and vaccinations in the region.

scholarly journals Recombinant envelope protein (rgp90) ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion

2012 ◽  
Vol 180 (1-2) ◽  
pp. 62-67 ◽  
Author(s):  
Jenner K.P. Reis ◽  
Rejane S. Diniz ◽  
João P.A. Haddad ◽  
Isabella B.F. Ferraz ◽  
Alex F. Carvalho ◽  
...  
Keyword(s):  
Envelope Protein ◽  
Equine Infectious Anemia Virus ◽  
Agar Gel ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Recombinant Envelope Protein

scholarly journals Comparison of Diagnostic Tests for the Detection of Equine Infectious Anemia Antibody

1989 ◽  
Vol 1 (1) ◽  
pp. 50-52 ◽  
Author(s):  
Tatsuo Matsushita ◽  
Lyndal K. Hesterberg ◽  
James P. Porter ◽  
Barbara J. Smith ◽  
Louis E. Newman
Keyword(s):  
Western Blot ◽  
Diagnostic Tests ◽  
Equine Infectious Anemia Virus ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Veterinary Services ◽  
Agar Gel ◽  
Equine Infectious Anemia ◽  
Low Levels ◽  
Anemia Virus

Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.

scholarly journals Development of a Fluorescence Polarization-Based Diagnostic Assay for Equine Infectious Anemia Virus

2000 ◽  
Vol 38 (5) ◽  
pp. 1854-1859 ◽  
Author(s):  
Sarah Burroughs Tencza ◽  
Kazi R. Islam ◽  
Vandana Kalia ◽  
Mohammad S. Nasir ◽  
Michael E. Jolley ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Equine Infectious Anemia Virus ◽  
Transmembrane Protein ◽  
Diagnostic Assay ◽  
Serum Samples ◽  
Diagnostic Laboratory ◽  
Peptide Antigen ◽  
Peptide Probe ◽  
Equine Infectious Anemia ◽  
Anemia Virus

The control of equine infectious anemia virus (EIAV) infections of horses has been over the past 20 years based primarily on the identification and elimination of seropositive horses, predominantly by a standardized agar gel immunodiffusion (AGID) assay in centralized reference laboratories. This screening for EIAV-seropositive horses has been to date hindered by the lack of a rapid diagnostic format that can be easily employed in the field. We describe here the development of a rapid solution-phase assay for the presence of serum antibodies to EIAV based on fluorescence polarization (FP) (patent pending). Peptides derived from antigenic regions of EIAV core and envelope proteins were initially screened for their utility as probes in an FP assay to select the best peptide antigen candidates. The FP assay was optimized to detect the presence of EIAV-specific antibodies by a change in the FP of a fluorescein-labeled immunoreactive peptide diagnostic antigen. The most sensitive and specific peptide probe was a peptide corresponding to the immunodominant region of the EIAV transmembrane protein, gp45. This probe was tested for its reactivity in the optimized FP assay with 151 AGID-positive horse sera and 106 AGID-negative serum samples. The results of these studies demonstrated that the FP assay reactivity correlated with reported AGID results in 106 of 106 negative serum samples (100% specificity) and in 135 of 151 positive serum samples (89.4% sensitivity). The FP assay was also found to have a very low background reactivity and to readily detect antibodies produced early in infection (≤3 weeks postinfection). The developed EIAV FP assay is rapid (5 to 20 min) and simple to perform and is equally suitable for use both in the field and in the diagnostic laboratory, thus providing the basis of an improved commercial diagnostic assay for EIAV infection of horses.

scholarly journals Western Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies

2007 ◽  
Vol 14 (12) ◽  
pp. 1646-1648 ◽  
Author(s):  
I. Alvarez ◽  
G. Gutierrez ◽  
E. Ostlund ◽  
M. Barrandeguy ◽  
K. Trono
Keyword(s):  
Western Blot ◽  
Capsid Protein ◽  
Diagnostic Tool ◽  
Equine Infectious Anemia Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agar Gel ◽  
Western Blot Assay ◽  
Specific Antibodies ◽  
Equine Infectious Anemia ◽  
Anemia Virus

ABSTRACT We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.

scholarly journals Equine infectious anemia on Marajo Island at the mouth of the Amazon river

2015 ◽  
Vol 35 (12) ◽  
pp. 947-950 ◽  
Author(s):  
Nayra F.Q.R. Freitas ◽  
Carlos M.C. Oliveira ◽  
Rômulo C. Leite ◽  
Jenner K.P. Reis ◽  
Fernanda G. Oliveira ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Serological Survey ◽  
Santa Cruz ◽  
Agar Gel ◽  
Incurable Disease ◽  
Island State ◽  
Equine Infectious Anemia ◽  
Marajó Island ◽  
Males And Females ◽  
Anemia Virus

Abstract: Equine infectious anemia (EIA) is a transmissible and incurable disease caused by a lentivirus, the equine infectious anemia virus (EIAV). There are no reports in the literature of this infection in Equidae on Marajo Island. The objective of this study was to diagnose the disease in the municipalities of Cachoeira do Arari, Salvaterra, Santa Cruz do Arari and Soure, on Marajó Island, state of Pará, Brazil. For serological survey samples were collected from 294 horses, over 5-month-old, males and females of puruca and marajoara breeds and from some half-breeds, which were tested by immunodiffusion in Agar gel (AGID). A prevalence of 46.26% (136/294) positive cases was found. EIA is considered endemic in the municipalities studied, due to the ecology of the region with a high numbered population of bloodsucking insect vectors and the absence of official measures for the control of the disease.

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