scholarly journals Expression of the Glycoprotein E2 of the Classical Swine Fever Virus in Escherichia coli.

1998 ◽  
Vol 60 (4) ◽  
pp. 541-544 ◽  
Author(s):  
Min-Liang WONG ◽  
Jau-Jin LIU ◽  
Yu-sun CHANG ◽  
Tien-Jye CHANG
Keyword(s):  
Escherichia Coli ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Glycoprotein E2

Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus

2008 ◽  
Vol 153 (8) ◽  
pp. 1593-1598 ◽  
Author(s):  
Y. Qi ◽  
L. C. Liu ◽  
B. Q. Zhang ◽  
Z. Shen ◽  
J. Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Responses ◽  
Neutralizing Epitope ◽  
Glycoprotein E2

Efficacy and stability of a subunit vaccine based on glycoprotein E2 of classical swine fever virus

1999 ◽  
Vol 66 (2) ◽  
pp. 101-114 ◽  
Author(s):  
A Bouma ◽  
A.J de Smit ◽  
E.P de Kluijver ◽  
C Terpstra ◽  
R.J.M Moormann
Keyword(s):  
Classical Swine Fever Virus ◽  
Subunit Vaccine ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
A Subunit ◽  
Glycoprotein E2

scholarly journals Optimal Conditions for the Expression of Glycoprotein E2 of Classical Swine Fever Virus using Baculovirus in Insect Cells

2014 ◽  
Vol 29 (2) ◽  
pp. 207-213
Author(s):  
Sung Min Bae ◽  
Seung Hee Lee ◽  
Won Suk Kwak ◽  
Yong Oh Ahn ◽  
Tae Young Shin ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Insect Cells ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Optimal Conditions ◽  
Glycoprotein E2

scholarly journals P108 and T109 on E2 Glycoprotein Domain I Are Critical for the Adaptation of Classical Swine Fever Virus to Rabbits but Not for Virulence in Pigs

2020 ◽  
Vol 94 (17) ◽  
Author(s):  
Libao Xie ◽  
Yuying Han ◽  
Yuteng Ma ◽  
Mengqi Yuan ◽  
Weike Li ◽  
...  
Keyword(s):  
Viral Entry ◽  
Classical Swine Fever Virus ◽  
Molecular Basis ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Viral Adaptation ◽  
Glycoprotein E2 ◽  
Domain I ◽  
Highly Virulent

ABSTRACT The classical swine fever virus (CSFV) live attenuated vaccine C-strain is adaptive to rabbits and attenuated in pigs, in contrast with the highly virulent CSFV Shimen strain. Previously, we demonstrated that P108 and T109 on the E2 glycoprotein (E2P108-T109) in domain I (E2DomainI) rather than R132, S133, and D191 in domain II (E2DomainII) determine C-strain’s adaptation to rabbits (ATR) (Y. Li, L. Xie, L. Zhang, X. Wang, C. Li, et al., Virology 519:197–206, 2018). However, it remains elusive whether these critical amino acids affect the ATR of the Shimen strain and virulence in pigs. In this study, three chimeric viruses harboring E2P108-T109, E2DomainI, or E2DomainII of C-strain based on the non-rabbit-adaptive Shimen mutant vSM-HCLVErns carrying the Erns glycoprotein of C-strain were generated and evaluated. We found that E2P108-T109 or E2DomainI but not E2DomainII of C-strain renders vSM-HCLVErns adaptive to rabbits, suggesting that E2P108-T109 in combination with the Erns glycoprotein (E2P108-T109-Erns) confers ATR on the Shimen strain, creating new rabbit-adaptive CSFVs. Mechanistically, E2P108-T109-Erns of C-strain mediates viral entry during infection in rabbit spleen lymphocytes, which are target cells of C-strain. Notably, pig experiments showed that E2P108-T109-Erns of C-strain does not affect virulence compared with the Shimen strain. Conversely, the substitution of E2DomainII and Erns of C-strain attenuates the Shimen strain in pigs, indicating that the molecular basis of the CSFV ATR and that of virulence in pigs do not overlap. Our findings provide new insights into the mechanism of adaptation of CSFV to rabbits and the molecular basis of CSFV adaptation and attenuation. IMPORTANCE Historically, live attenuated vaccines produced by blind passage usually undergo adaptation in cell cultures or nonsusceptible hosts and attenuation in natural hosts, with a classical example being the classical swine fever virus (CSFV) lapinized vaccine C-strain, which was developed by hundreds of passages in rabbits. However, the mechanism of viral adaptation to nonsusceptible hosts and the molecular basis for viral adaptation and attenuation remain largely unknown. In this study, we demonstrated that P108 and T109 on the E2 glycoprotein together with the Erns glycoprotein of the rabbit-adaptive C-strain confer adaptation to rabbits on the highly virulent CSFV Shimen strain by affecting viral entry during infection but do not attenuate the Shimen strain in pigs. Our results provide vital information on the different molecular bases of CSFV adaptation to rabbits and attenuation in pigs.

Production of Classical Swine Fever Virus Envelope Glycoprotein E2 as Recombinant Polyhedra in Baculovirus-Infected Silkworm Larvae

2011 ◽  
Vol 50 (3) ◽  
pp. 211-220 ◽  
Author(s):  
Kwang Sik Lee ◽  
Mi Ri Sohn ◽  
Bo Yeon Kim ◽  
Young Moo Choo ◽  
Soo Dong Woo ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Envelope Glycoprotein ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Silkworm Larvae ◽  
Virus Envelope ◽  
Glycoprotein E2

scholarly journals Comprehensive analysis of the codon usage patterns in the envelope glycoprotein E2 gene of the classical swine fever virus

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0183646 ◽  
Author(s):  
Ye Chen ◽  
Xinxin Li ◽  
Xiaojuan Chi ◽  
Song Wang ◽  
Yanmei Ma ◽  
...  
Keyword(s):  
Codon Usage ◽  
Classical Swine Fever Virus ◽  
Envelope Glycoprotein ◽  
Classical Swine Fever ◽  
Comprehensive Analysis ◽  
Fever Virus ◽  
E2 Gene ◽  
Usage Patterns ◽  
Glycoprotein E2

Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System

2010 ◽  
Vol 74 (7) ◽  
pp. 1343-1349 ◽  
Author(s):  
Chi-Ming WU ◽  
Shih-Ling HSUAN ◽  
Zeng-Weng CHEN ◽  
Tzyy-Rong JINN ◽  
Chienjin HUANG ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Expression System ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Virus Glycoprotein ◽  
Glycoprotein E2

scholarly journals Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Qiang Wei ◽  
Yilin Bai ◽  
Yapeng Song ◽  
Yunchao Liu ◽  
Wei Yu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Classical Swine Fever Virus ◽  
Expression System ◽  
Classical Swine Fever ◽  
Neutralizing Antibody ◽  
Baculovirus Expression System ◽  
Viral Envelope ◽  
Fever Virus ◽  
Complete Protection ◽  
Glycoprotein E2

AbstractClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and Erns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and Erns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-Erns, or their combination (CSFV-E2 + Erns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + Erns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the Erns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the Erns alone is not able to induce a protective immune response. Taken together, while the Erns could not confer protection against CSFV, E2 and E2 + Erns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.

Sign in / Sign up

Export Citation Format

Share Document