scholarly journals Feline infectious peritonitis virus antibody test using enzyme-linked immunosorbent assay.

1987 ◽  
Vol 49 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Takuo ISHIDA ◽  
Tsukimi WASHIZU ◽  
Jun FUKUOKA ◽  
Kazushige TORIYABE ◽  
Tomiya UCHINO ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Antibody ◽  
Feline Infectious Peritonitis Virus ◽  
Feline Infectious Peritonitis

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

scholarly journals Development of Rapid Immunochromatographic Test with Recombinant NcSAG1 for Detection of Antibodies to Neospora caninum in Cattle

2005 ◽  
Vol 12 (7) ◽  
pp. 885-887 ◽  
Author(s):  
Min Liao ◽  
Shoufa Zhang ◽  
Xuenan Xuan ◽  
Guohong Zhang ◽  
Xiaohong Huang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Reliable Method ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Serum Samples ◽  
Immunofluorescent Antibody Test ◽  
Good Agreement

ABSTRACT An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.

scholarly journals AVIAN INFLUENZA ANTIBODY RESPONSE IN PIGLETS THATAREGIVEN ESCHERICHIA COLI–AVIAN INFLUENZA VACCINES

2021 ◽  
Vol 10 (1) ◽  
pp. 61-70
Author(s):  
Audrey Febiannya Putri Bhaskara ◽  
I Gusti Ngurah Kade Mahardika ◽  
I Nyoman Suartha
Keyword(s):  
Escherichia Coli ◽  
Avian Influenza ◽  
Optical Density ◽  
Antibody Test ◽  
Influenza Vaccines ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Antibody ◽  
E Coli ◽  
Test Kit ◽  
Virus Influenza

Babi berperan penting dalam ekologi virus influenza, karena babi dapat berperan sebagai wahana untuk reasorsi virus influenza dari unggas dan mamalia. Vaksinasi dengan antigen influenza universal, yaitu nukleoprotein, dapat menurunkan peluang babi dalam memunculkan virus influenza baru. Penelitian ini bertujuan untuk mengentahui respons antibodi dari vaksinasi dengan nukleoprotein rekombinan - Escherichia coli. Sebanyak 12 anak babi landrace dari tiga induk yang berbeda dipilih secara acak. Enam ekor divaksinasi dengan vaksin nucleoprotein-E. coli pada umur tujuh hari dan diulang pada umur 21 hari. Enam ekor tidak divaksinasi. Serum diambil pada umur 35 hari. Nilai optical density (OD) antibodi terhadap nukleoprotein diuji dengan teknik Enzyme-Linked Immunosorbent Assay (ELISA) dengan menggunakan Kit ELISA komersial, Avian Influenza Virus Antibody Test Kit. Hasil penelitian menunjukkan bahwa nilai Optical Density rata-rata babi yang divaksinasi (0,367) secara statistika nyata lebih tinggi dibandingkan dengan yang tidak divaksinasi (0,054). Vaksin rekombinan nucleoprotein-E. coli yang dicobakan mampu meningkatan antibodi terhadap virus avian influenza pada anak babi.

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