Hyaluronic acid filler vascular complication management: an updated and easy-to-follow emergency protocol

With the breadth and variety of hyaluronic acid hydrogels available, it can be challenging to understand the evolving product characteristics and associated terminology. Similarly, different hyaluronic acid hydrogels can share the same indication, and yet consist of different rheological and physiochemical properties. In this paper, hyaluronic acid biophysical properties, such as molecular weights, stabilisation (crosslinking), modification and hyaluronic acid concentration, are explored in relation to findings from current literature. The significance for these specific properties is explored in relation to specific indications and anti-ageing benefits.

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Clinicians’ Perspectives on the Use of Intra-Articular Hyaluronic Acid as a Treatment for Knee Osteoarthritis: A North American, Multidisciplinary Survey

Clinical Medicine Insights Arthritis and Musculoskeletal Disorders ◽

10.4137/cmamd.s34496 ◽

2016 ◽

Vol 9 ◽

pp. CMAMD.S34496 ◽

Cited By ~ 3

Author(s):

Jeffrey Rosen ◽

Victoria Avram ◽

Anke Fierlinger ◽

Faizan Niazi ◽

Parag Sancheti ◽

...

Keyword(s):

Hyaluronic Acid ◽

Knee Osteoarthritis ◽

Patient Outcomes ◽

Current Literature ◽

Early Stage ◽

Sodium Hyaluronate ◽

Cross Linking ◽

Product Characteristics ◽

Knee Oa ◽

Orthopedic Surgeons

Introduction This study aims to describe the perceptions of orthopedic surgeons on the efficacy of intra-articular hyaluronic acid (IA-HA), the influence of IA-HA product characteristics on its efficacy, and to identify patterns and factors related to the use of IA-HA. Additionally, this study examines factors that influence IA-HA brand selection, focusing on Euflexxa¯ (1% sodium hyaluronate). Methods We developed survey questions by reviewing the current literature and consulting with experts on the use of IA-HA in the management of knee osteoarthritis (OA). The survey included questions on demographics, previous experience with knee OA treatment, opinions on different treatment methods, and where information regarding treatments is obtained. Additionally, questions specific to opinions regarding IA-HA and the reasoning behind these opinions were asked. Results A total of 117 orthopedic surgeons and physicians completed the survey. IA-HA is most frequently prescribed to patients with early-stage (82%) or mid-stage (82.8%) OA, while fewer orthopedic surgeons and physicians use IA-HA for patients with late-stage OA (57.4%). Respondents were generally uncertain of the effects that intrinsic characteristics, such as molecular weight, cross-linking, and production process, had on patient outcomes. Respondents typically use their own clinical experience and results as a deciding factor in utilizing IA-HA treatment, as well as in choosing an IA-HA brand. Conclusion Uncertainty regarding the efficacy of IA-HA treatments is likely due to inconsistency within clinical guidelines and the current literature. Additional research investigating the efficacy of IA-HA treatment and how product characteristics affect outcome and safety is required to provide clarity to the controversy surrounding IA-HA treatment for knee OA.

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Anti-coagulant Activity of Isolated and Partially Characterized Proteoglycans and Glycosaminoglycans from African Night Crawler (Eudrilus eugeniae Kinberg)

KIMIKA ◽

10.26534/kimika.v26i2.31-38 ◽

2015 ◽

Vol 26 (2) ◽

pp. 31-38

Author(s):

Mia Clare Marie L. Bercansil ◽

Miko Lorenzo J. Belgado

Keyword(s):

Hyaluronic Acid ◽

Infrared Spectroscopy ◽

Enzymatic Hydrolysis ◽

Gel Filtration ◽

Acetate Buffer ◽

Eudrilus Eugeniae ◽

Gel Filtration Chromatography ◽

Molecular Weights ◽

Coagulant Activity ◽

Hydrolysis Of

Proteoglycans and glycosaminoglycans were isolated from African night crawler (Eudrilus eugeniae Kinberg) and partially characterized proteoglycans (3.04 % of lyophilized worm) were liberated from the defatted and depurinated worm samples by dissociative method using 4M urea in acetate buffer. Glycosaminoglycans (12.47% of proteoglycan extract) were extracted using enzymatic hydrolysis of the proteoglycan extract with papain. Gel filtration chromatography using Sepharose CL-4B was used to purify and estimate the molecular weights of the proteoglycan and glycosaminoglycan fractions. Three proteoglycan fractions PGF1, PGF2 and PGF3 with estimated molecular weigths 860 kDa, 181 kDa and 3 kDa, respectively were identified as monitored by the Bradford and modified carbazole assay. Two glycosaminoglycan fractions - GF1 (MW = 860 kDa) and GF2 (MW=140 kDa) were identified using the modified carbazole assay. Infrared spectroscopy of the GF1 and GF2 showed the possible identities of the fractions. GF1 may be a hyaluronic acid and GF2 is possibly chondroitin. Anti-coagulant assay for the extracts and fractions revealed that the glycosaminoglycan isolate has anti-coagulant activity but not the GF1 and GF2 fractions individually.

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Expression Hyaluronic Acid Concentration

Biomedical Safety & Standards ◽

10.1097/01.bmsas.0000455054.11181.1a ◽

2014 ◽

Vol 44 (18) ◽

pp. 139

Keyword(s):

Hyaluronic Acid ◽

Acid Concentration

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Serum hyaluronic acid concentration predicts the progression of joint space narrowing in normal knees and established knee osteoarthritis – a five-year prospective cohort study

Arthritis Research & Therapy ◽

10.1186/s13075-015-0793-0 ◽

2015 ◽

Vol 17 (1) ◽

Cited By ~ 19

Author(s):

Eiji Sasaki ◽

Eiichi Tsuda ◽

Yuji Yamamoto ◽

Shugo Maeda ◽

Ryo Inoue ◽

...

Keyword(s):

Cohort Study ◽

Hyaluronic Acid ◽

Knee Osteoarthritis ◽

Acid Concentration ◽

Prospective Cohort Study ◽

Prospective Cohort ◽

Joint Space Narrowing ◽

Joint Space

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Analysis of hyaluronic acid concentration in rat vocal folds during estral and gravidic puerperal cycles

Brazilian Journal of Otorhinolaryngology ◽

10.1016/s1808-8694(15)30513-9 ◽

2009 ◽

Vol 75 (5) ◽

pp. 654-659

Author(s):

José Eduardo de Sá Pedroso ◽

Osíris Camponês do Brasil ◽

João Roberto Maciel Martins ◽

Helena Bociane Nader ◽

Manuel de Jesus Simões

Keyword(s):

Hyaluronic Acid ◽

Acid Concentration ◽

Vocal Folds

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In vitro synthesis of glycosaminoglycans in endocrine ophthalmopathy

Acta Endocrinologica ◽

10.1530/acta.0.1270397 ◽

1992 ◽

Vol 127 (5) ◽

pp. 397-402 ◽

Cited By ~ 6

Author(s):

G Kahaly ◽

C Stover ◽

J Beyer ◽

E Otto

Keyword(s):

Hyaluronic Acid ◽

Acid Concentration ◽

Cetylpyridinium Chloride ◽

Acid Synthesis ◽

Cell Mediated Immunity ◽

Glycosaminoglycan Synthesis ◽

In Vitro Synthesis ◽

Significant Difference ◽

Endocrine Ophthalmopathy

The effects of humoral and cell-mediated immunity on the glycosaminoglycan synthesis of retrobulbar fibroblasts was evaluated in patients with endocrine ophthalmopathy. After incubation with IgG and sera, secreted glycosaminoglycans, radiolabeled with D-6-3H-glucosamine and 35sulfate, were precipitated with cetylpyridinium chloride and ethanol. Hyaluronic acid synthesis of human retrobulbar fibroblasts after incubation with sera and IgG and after co-culture with lymphocytes was assessed by means of a radiometric test. Patients' IgG, compared to controls', accounted for a higher secretory stimulation of porcine retrobulbar fibroblasts (as measured by cetylpyridinium chloride precipitation) after 24 and 48 h. Contrasting with 24 h incubation time, glycosaminoglycan values after 48 h were increased two to threefold. Patients' and controls' sera caused earlier and stronger, yet indistinguishable glycosaminoglycan production. Non-sulfated hyaluronic acid was the preponderant glycosaminoglycan secreted into the media by retrobulbar fibroblasts. As assessed with the radiometric test, incubation with patients' and controls' sera and IgG did not reveal a significant difference in stimulating the hyaluronic synthesis of patients' and controls' retrobulbar fibroblasts. When measuring the hyaluronic acid synthesis of controls' and patients' retrobulbar fibroblasts after co-cultivation of lymphocytes, however, patients' lymphocytes had a marked ability to increase the hyaluronic acid concentration compared to controls' lymphocytes. The hyaluronic acid concentration after incubation of a patient's retrobulbar fibroblasts with autologous lymphocytes was markedly more elevated than the intrinsic hyaluronic acid production of retrobulbar fibroblasts. In conclusion, though a significant in vitro influence of patients' IgG and sera on the glycosaminoglycan release of both porcine and human (patients' as well as controls') retrobulbar fibroblasts could not be observed in this study, the indications of a marked stimulatory influence of lymphocytes on the hyaluronic acid secretion of retrobulbar fibroblasts demand further investigation.

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