The analysis of nitric oxide production behavior in mouse isolated osteocytes in oscillatory shear stress flow

2018 ◽  
Vol 2018.30 (0) ◽  
pp. 2A07
Author(s):  
Ryotaro ASHIYA ◽  
Junko SUNAGA ◽  
Taiji ADACHI
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Oscillatory Shear ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Production Behavior ◽  
Oscillatory Shear Stress ◽  
Stress Flow

Shear-stress mediated nitric oxide production within red blood cells: A dose-response

2019 ◽  
Vol 71 (2) ◽  
pp. 203-214 ◽  
Author(s):  
Jarod T. Horobin ◽  
Nobuo Watanabe ◽  
Masaya Hakozaki ◽  
Surendran Sabapathy ◽  
Michael J. Simmonds
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Red Blood Cells ◽  
Dose Response ◽  
Blood Cells ◽  
Nitric Oxide Production ◽  
Oxide Production

scholarly journals In Silico Modeling of Shear-Stress-Induced Nitric Oxide Production in Endothelial Cells through Systems Biology

2013 ◽  
Vol 104 (10) ◽  
pp. 2295-2306 ◽  
Author(s):  
Andrew Koo ◽  
David Nordsletten ◽  
Renato Umeton ◽  
Beracah Yankama ◽  
Shiva Ayyadurai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Systems Biology ◽  
In Silico ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
In Silico Modeling

Gene Expression of Endothelial Cells under Pulsatile Non-Reversing vs. Steady Shear Stress; Comparison of Nitric Oxide Production

2008 ◽  
Vol 36 (4) ◽  
pp. 571-579 ◽  
Author(s):  
Andrew Yee ◽  
Kami A. Bosworth ◽  
Daniel E. Conway ◽  
Suzanne G. Eskin ◽  
Larry V. McIntire
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Steady Shear ◽  
Nitric Oxide Production ◽  
Oxide Production

Impaired shear stress-induced nitric oxide production through decreased NOS phosphorylation contributes to age-related vascular stiffness

2006 ◽  
Vol 101 (6) ◽  
pp. 1751-1759 ◽  
Author(s):  
Kevin G. Soucy ◽  
Sungwoo Ryoo ◽  
Alexandre Benjo ◽  
Hyun Kyo Lim ◽  
Gaurav Gupta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Vascular Stiffness ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Age Related

Plaque-prone hemodynamics impair endothelial function in pig carotid arteries

2006 ◽  
Vol 290 (6) ◽  
pp. H2320-H2328 ◽  
Author(s):  
Veronica Gambillara ◽  
Céline Chambaz ◽  
Gabriela Montorzi ◽  
Sylvain Roy ◽  
Nikos Stergiopulos ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Dysfunction ◽  
Oscillatory Shear ◽  
Enos Gene ◽  
Low Shear Stress ◽  
Oscillatory Shear Stress ◽  
Low Shear

Hemodynamic forces play an active role in vascular pathologies, particularly in relation to the localization of atherosclerotic lesions. It has been established that low shear stress combined with cyclic reversal of flow direction (oscillatory shear stress) affects the endothelial cells and may lead to an initiation of plaque development. The aim of the study was to analyze the effect of hemodynamic conditions in arterial segments perfused in vitro in the absence of other stimuli. Left common porcine carotid segments were mounted into an ex vivo arterial support system and perfused for 3 days under unidirectional high and low shear stress (6 ± 3 and 0.3 ± 0.1 dyn/cm2) and oscillatory shear stress (0.3 ± 3 dyn/cm2). Bradykinin-induced vasorelaxation was drastically decreased in arteries exposed to oscillatory shear stress compared with unidirectional shear stress. Impaired nitric oxide-mediated vasodilation was correlated to changes in both endothelial nitric oxide synthase (eNOS) gene expression and activation in response to bradykinin treatment. This study determined the flow-mediated effects on native tissue perfused with physiologically relevant flows and supports the hypothesis that oscillatory shear stress is a determinant factor in early stages of atherosclerosis. Indeed, oscillatory shear stress induces an endothelial dysfunction, whereas unidirectional shear stress preserves the function of endothelial cells. Endothelial dysfunction is directly mediated by a downregulation of eNOS gene expression and activation; consequently, a decrease of nitric oxide production and/or bioavailability occurs.

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