D213 Ice growth and temperature field in solution of antifreeze protein and sodium chloride flowing in a mini-channel

2013 ◽  
Vol 2013 (0) ◽  
pp. 339-340
Author(s):  
Kazuya Taira ◽  
Yoshimichi Hagiwara ◽  
Yosuke Onishi ◽  
Yoshiaki Nakagawa
Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 306
Author(s):  
Kazuya Taira ◽  
Tomonori Waku ◽  
Yoshimichi Hagiwara

The control of ice growth inside channels of aqueous solution flows is important in numerous fields, including (a) cold-energy transportation plants and (b) the preservation of supercooled human organs for transplantation. A promising method for this control is to add a substance that influences ice growth in the flows. However, limited results have been reported on the effects of such additives. Using a microscope, we measured the growth of ice from one sidewall toward the opposite sidewall of a mini-channel, where aqueous solutions of sodium chloride and antifreeze protein flowed. Our aim was to considerably suppress ice growth by mixing the two solutes. Inclined interfaces, the overlapping of serrated interfaces, and interfaces with sharp and flat tips were observed in the cases of the protein-solution, salt-solution, and mixed-solution flows, respectively. In addition, it was found that the average interface velocity in the case of the mixed-solution flow was the lowest and decreased by 64% compared with that of pure water. This significant suppression of the ice-layer growth can be attributed to the synergistic effects of the ions and antifreeze protein on the diffusion of protein.


Cryobiology ◽  
2009 ◽  
Vol 59 (3) ◽  
pp. 372
Author(s):  
Manabu Takamichi ◽  
Yoshiyuki Nishimiya ◽  
Ai Miura ◽  
Sakae Tsuda

Biomolecules ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 70 ◽  
Author(s):  
Yuki Takeshita ◽  
Tomonori Waku ◽  
Peter W. Wilson ◽  
Yoshimichi Hagiwara

The control of ice growth in ice slurry is important for many fields, including (a) the cooling of the brain during cardiac arrest, (b) the storage and transportation of fresh fish and fruits, and (c) the development of distributed air-conditioning systems. One of the promising methods for the control is to use a substance such as antifreeze protein. We have observed and report here growth states of ice particles in both quiescent and flowing aqueous solutions of winter flounder antifreeze proteins in mini-channels with a microscope. We also measured ice growth rates. Our aim was to improve the levels of ice growth inhibition by subjecting the antifreeze protein solution both to preheating and to concentrating by ultrafiltration. We have found that the ice growth inhibition by the antifreeze protein decreased in flowing solutions compared with that in quiescent solutions. In addition, unlike unidirectional freezing experiments, the preheating of the antifreeze protein solution reduced the ice growth inhibition properties. This is because the direction of flow, containing HPLC6 and its aggregates, to the ice particle surfaces can change as the ice particle grows, and thus the probability of interaction between HPLC6 and ice surfaces does not increase. In contrast to this, ultrafiltration after preheating the solution improved the ice growth inhibition. This may be due to the interaction between ice surfaces and many aggregates in the concentrates.


2015 ◽  
Vol 2015 (0) ◽  
pp. _G133-1_-_G133-2_
Author(s):  
Kazuhide Arai ◽  
Masato Nishi ◽  
Takuya Miyamoto ◽  
Yoshimichi Hagiwara

1974 ◽  
Vol 96 (3) ◽  
pp. 415-420 ◽  
Author(s):  
T. E. Cooper ◽  
W. K. Petrovic

Liquid crystals, a material that exhibits brilliant changes in color over narrow temperature bands, have been successfully used to study the temperature field that is produced by a cryosurgical cannula (cryoprobe). Cryoprobe tip temperatures ranging from −36 to −117 C were used to produce frozen regions in a clear gel. Experimental results compare within experimental uncertainty with results of a one-dimensional analytical solution for predicting ice growth rates.


Cryobiology ◽  
2013 ◽  
Vol 67 (3) ◽  
pp. 438
Author(s):  
Maddalena Bayer-Giraldi ◽  
Ilka Weikusat ◽  
Cornelia Isert

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