Biodeposition of Copper Crystals by Marine Microorganisms

2017 ◽  
Vol 2017.66 (0) ◽  
pp. 801
Author(s):  
Masahito MATSUI ◽  
Takao NOGUCHI ◽  
Kenichi MURAI ◽  
Yuichi NAKAMURA
2020 ◽  
Vol 9 (1) ◽  
pp. 3
Author(s):  
Saskia Rughöft ◽  
Nico Jehmlich ◽  
Tony Gutierrez ◽  
Sara Kleindienst

The application of chemical dispersants during marine oil spills can affect the community composition and activity of marine microorganisms. Several studies have indicated that certain marine hydrocarbon-degrading bacteria, such as Marinobacter spp., can be inhibited by chemical dispersants, resulting in lower abundances and/or reduced biodegradation rates. However, a major knowledge gap exists regarding the mechanisms underlying these physiological effects. Here, we performed comparative proteomics of the Deepwater Horizon isolate Marinobacter sp. TT1 grown under different conditions. Strain TT1 received different carbon sources (pyruvate vs. n-hexadecane) with and without added dispersant (Corexit EC9500A). Additional treatments contained crude oil in the form of a water-accommodated fraction (WAF) or chemically-enhanced WAF (CEWAF; with Corexit). For the first time, we identified the proteins associated with alkane metabolism and alginate biosynthesis in strain TT1, report on its potential for aromatic hydrocarbon biodegradation and present a protein-based proposed metabolism of Corexit components as carbon substrates. Our findings revealed that Corexit exposure affects hydrocarbon metabolism, chemotactic motility, biofilm formation, and induces solvent tolerance mechanisms, like efflux pumps, in strain TT1. This study provides novel insights into dispersant impacts on microbial hydrocarbon degraders that should be taken into consideration for future oil spill response actions.


Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 256
Author(s):  
Yehui Gang ◽  
Tae-Yang Eom ◽  
Svini Dileepa Marasinghe ◽  
Youngdeuk Lee ◽  
Eunyoung Jo ◽  
...  

Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism–derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.


2021 ◽  
Vol 28 (1) ◽  
pp. 224-231
Author(s):  
Fuad Ameen ◽  
Saleh AlNadhari ◽  
Ali A. Al-Homaidan

1953 ◽  
Vol 1 (1) ◽  
pp. 75-78 ◽  
Author(s):  
W.G Burgers ◽  
J.C Meijs ◽  
T.J Tiedema

BMC Genomics ◽  
2011 ◽  
Vol 12 (Suppl 3) ◽  
pp. S15 ◽  
Author(s):  
Atsushi Ogura ◽  
Mengjie Lin ◽  
Yuya Shigenobu ◽  
Atushi Fujiwara ◽  
Kazuho Ikeo ◽  
...  

1996 ◽  
Vol 14 (1) ◽  
pp. 6-12 ◽  
Author(s):  
Chiaki Kato ◽  
Akira Inoue ◽  
Koki Horikoshi

2021 ◽  
Vol 73 (2) ◽  
pp. 209-224
Author(s):  
Maxwell Uphaus

Abstract Where beaches and harbors have frequently been taken to signify openness and intermingling, a different coastal setting, the cliffs of Dover, overtly bespeaks opposition and closure. Demarcating the British coast at its closest point to continental Europe, the cliffs often stand for Britain’s supposedly elemental insularity. However, the chalk composing the cliffs makes them, in their own way, as malleable and permeable as a beach. I argue that poems by Matthew Arnold, W. H. Auden, and Daljit Nagra contest the cliffs’ association with an exclusive Britishness by focusing on their material composition. In these poems, the cliffs’ chalk—formed by fossilized marine microorganisms at a time when what would become Britain was at the bottom of a prehistoric sea—attests to Britain’s geohistorical contingency. Arnold, Auden, and Nagra use this chalk geology to develop a new model of British identity as contingent, permeable, and linked with the wider world. In these poems, that is, Dover’s cliffs collapse oppositions rather than enforcing them: they blur the lines between Britain and the world, past and present, organic and inorganic, human history and geological history. The literature of the Dover cliffs thus highlights the revisionary potential of this distinctive kind of littoral space.


2010 ◽  
Vol 73 (8) ◽  
pp. 1529-1532 ◽  
Author(s):  
JUN KUDAKA ◽  
TORU HORII ◽  
KOJI TAMANAHA ◽  
KIYOMASA ITOKAZU ◽  
MASAJI NAKAMURA ◽  
...  

The enumeration and evaluation of the activity of marine bacteria are important in the food industry. However, detection of marine bacteria in seawater or seafood has not been easy. The Petrifilm aerobic count plate (ACP) is a ready-to-use alternative to the traditional enumeration media used for bacteria associated with food. The purpose of this study was to evaluate the usefulness of a simple detection and enumeration method utilizing the Petrifilm ACP for enumeration of aerobic marine bacteria from seawater and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80 seawater and 64 C. lentillifera samples. With sterile seawater as the diluent, a close correlation was observed between the method utilizing Petrifilm ACP and that utilizing the conventional marine agar (r = 0.98 for seawater and 0.91 for C. lentillifera). The Petrifilm ACP method was simpler and less time-consuming than the conventional method. These results indicate that Petrifilm ACP is a suitable alternative to conventional marine agar for enumeration of marine microorganisms in seawater and C. lentillifera samples.


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