scholarly journals In vitro Assessment of Cytotoxic Activity of Bioactive Peptides from Momordica dioica and Solanum trilobatum against Human Colon Cancer Cells

2021 ◽  
Vol 14 (02) ◽  
pp. 1007-1018
Author(s):  
Rupachandra S ◽  
Mario Prateek Selvam ◽  
Nisha Muthukumaran ◽  
Sangamithra Senthilkumar ◽  
Sandhiya Vaidhyalingam ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Bioactive Peptides ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Cell Agglutination ◽  
Plant Peptides ◽  
Solanum Trilobatum

Plant peptides have gained attention in the medicinal field due to their high anti-microbial and anti-cancer properties. Various plant sources are being used to extract proteins and peptides to be used as a cure for a variety of diseases. The latest studies show that plant peptides are effective in the treatment of cancer due to their ability to preferentially bind to the receptors or membranes of cancer cells leading to tumour growth inhibition, cytotoxicity, decreased proliferation, and apoptosis. The peptides get internalized into the cells causing cancer cell agglutination and aggregation. In addition to acting as therapeutic agents, plant peptides are also used for the targeting of drugs specific to cancer cells. In this study, bioactive peptides were isolated from the seeds of Momordica dioica and leaves of Solanum trilobatum. They were screened and identified using HPLC and MALDI-TOF techniques. In this study, apoptosis was analyzed by the Hoechst 33342 staining method which detected the presence of condensed pycnotic nuclei in apoptotic COLO320DM and COLO205 colon cancer cellsat the maximum concentrations of 150 and 175 µg/mL of plant peptides.Further DCFH-DA staining indicated the intracellular ROS production in the treated COLO320DM and COLO205 colon cancer cells. Thus, the isolated bioactive plant peptides can be formulated towards the development of effective anticancer drugs for the treatment of colon cancer in humans.

scholarly journals P-0193 Transgelin Promotes The Invasiveness of Human Colon Cancer Cells in Vitro

2012 ◽  
Vol 23 ◽  
pp. iv85-iv86
Author(s):  
Ying Lin ◽  
Yuan-yuan Fang ◽  
Hong Su ◽  
Zhou Hui-Min ◽  
Qi-Kui Chen
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

scholarly journals In Vitro and in Vivo antitumor activity and the mechanism of siphonodictyal B in human colon cancer cells

2019 ◽  
Vol 8 (12) ◽  
pp. 5662-5672 ◽  
Author(s):  
Sonoko Chikamatsu ◽  
Ken Saijo ◽  
Hiroo Imai ◽  
Koichi Narita ◽  
Yoshifumi Kawamura ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
In Vivo Antitumor Activity ◽  
Human Colon Cancer Cells

scholarly journals NF-κB maintains the stemness of colon cancer cells by downregulating miR-195-5p/497–5p and upregulating MCM2

2020 ◽  
Vol 39 (1) ◽  
Author(s):  
Longgang Wang ◽  
Jinxiang Guo ◽  
Jin Zhou ◽  
Dongyang Wang ◽  
Xiuwen Kang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Tumor Models ◽  
Human Colon Cancer ◽  
Subcutaneous Tumor

Abstract Background Colon cancer represents one of the leading causes of gastrointestinal tumors in industrialized countries, and its incidence appears to be increasing at an alarming rate. Accumulating evidence has unveiled the contributory roles of cancer stem cells (CSCs) in tumorigenicity, recurrence, and metastases. The functions of NF-kappa B (NF-κB) activation on cancer cell survival, including colon cancer cells have encouraged us to study the role of NF-κB in the maintenance of CSCs in colon cancer. Methods Tumor samples and matched normal samples were obtained from 35 colon cancer cases. CSCs were isolated from human colon cancer cell lines, where the stemness of the cells was evaluated by cell viability, colony-forming, spheroid-forming, invasion, migration, and apoptosis assays. NF-κB activation was then performed in subcutaneous tumor models of CSCs by injecting lipopolysaccharides (LPS) i.p. Results We found that NF-κB activation could reduce the expression of miR-195-5p and miR-497-5p, where these two miRNAs were determined to be downregulated in colon cancer tissues, cultured colon CSCs, and LPS-injected subcutaneous tumor models. Elevation of miR-195-5p and miR-497-5p levels by their specific mimic could ablate the effects of NF-κB on the stemness of colon cancer cells in vivo and in vitro, suggesting that NF-κB could maintain the stemness of colon cancer cells by downregulating miR-195-5p/497–5p. MCM2 was validated as the target gene of miR-195-5p and miR-497-5p in cultured colon CSCs. Overexpression of MCM2 was shown to restore the stemness of colon cancer cells in the presence of miR-195-5p and miR-497-5p, suggesting that miR-195-5p and miR-497-5p could impair the stemness of colon cancer cells by targeting MCM2 in vivo and in vitro. Conclusions Our work demonstrates that the restoration of miR-195-5p and miR-497-5p may be a therapeutic strategy for colon cancer treatment in relation to NF-κB activation.

scholarly journals Anticancer potential of an ethanol extract of Asiasari radix against HCT-116 human colon cancer cells in vitro

2012 ◽  
Vol 5 (1) ◽  
pp. 305-310 ◽  
Author(s):  
SE-MI OH ◽  
JINHEE KIM ◽  
JUN LEE ◽  
JIN-MU YI ◽  
DAL-SEOK OH ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Ethanol Extract ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Hct 116 ◽  
Human Colon Cancer Cells
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