Enhanced Acetone-Butanol-Ethanol Production by Clostridium beijerinckii Using Biochar

Author(s):  
Xiao Sun ◽  
Hasan K. Atiyeh ◽  
Yinka A. Adesanya ◽  
Hailin Zhang ◽  
Christopher Okonkwo ◽  
...  
2015 ◽  
Vol 190 ◽  
pp. 332-338 ◽  
Author(s):  
Carolina Bellido ◽  
Celia Infante ◽  
Mónica Coca ◽  
Gerardo González-Benito ◽  
Susana Lucas ◽  
...  

2021 ◽  
Vol 8 (9) ◽  
pp. 201858
Author(s):  
Lihua Lin ◽  
Zhikai Zhang ◽  
Hongchi Tang ◽  
Yuan Guo ◽  
Bingqing Zhou ◽  
...  

A heterologous pathway for sucrose transport and metabolism was introduced into Clostridium beijerinckii to improve sucrose use for n -butanol production. The combined expression of StSUT1 , encoding a sucrose transporter from potato ( Solanum tuberosum ), and SUC2 , encoding a sucrose invertase from Saccharomyces cerevisiae , remarkably enhanced n -butanol production. With sucrose, sugarcane molasses and sugarcane juice as substrates, the C. beijerinckii strain harbouring StSUT1 and SUC2 increased acetone–butanol–ethanol production by 38.7%, 22.3% and 52.8%, respectively, compared with the wild-type strain. This is the first report to demonstrate enhanced sucrose fermentation due to the heterologous expression of a sucrose transporter and invertase in Clostridium . The metabolic engineering strategy used in this study can be widely applied in other microorganisms to enhance the production of high-value compounds from sucrose-based biomass.


2014 ◽  
Vol 49 (8) ◽  
pp. 1238-1244 ◽  
Author(s):  
Min Jiang ◽  
Jia-nan Chen ◽  
Ai-yong He ◽  
Hao Wu ◽  
Xiang-ping Kong ◽  
...  

2015 ◽  
Vol 50 (4) ◽  
pp. 614-622 ◽  
Author(s):  
Hao Wu ◽  
Ai-yong He ◽  
Xiang-ping Kong ◽  
Min Jiang ◽  
Xiao-peng Chen ◽  
...  

2012 ◽  
Vol 6 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Jie Zhang ◽  
Mingyu Wang ◽  
Mintian Gao ◽  
Xu Fang ◽  
Shinichi Yano ◽  
...  

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 185-191 ◽  
Author(s):  
Marek Drahokoupil ◽  
Petra Patáková

A solventogenic strain of Clostridium beijerinckii, NRRL B-598, was cultured for the production of butyric acid as the main fermentation product. However, unlike typical acetone-butanol-ethanol (ABE) fermentations, where pH is not regulated, in this study the pH was kept constant during fermentation. From the five pH values tested, 6.0, 6.5, 7.0, 7.5 and 8.0, pH 6.5 and 7.0 resulted in the highest concentrations of butyric acid, at 9.69 ± 0.09 g L–1 and 11.5 ± 0.39 g L–1, respectively. However, a low concentration of solvents, 1.8 ± 0.22 g L–1, was only reached at pH 7.0. These results are comparable with those from typical butyric acid producers, i.e. Clostridium butyricum and Clostridium tyrobutyricum strains. At pH 7.0, we succeeded in suppressing sporulation and prolonging the population viability, which was confirmed by flow cytometry combined with double fluorescence staining.


2020 ◽  
Vol 316 ◽  
pp. 123899
Author(s):  
Zhi-you Zhou ◽  
Shang-tian Yang ◽  
Curtis D. Moore ◽  
Qing-hua Zhang ◽  
Shuai-ying Peng ◽  
...  

2019 ◽  
Vol 95 (3) ◽  
pp. 614-620 ◽  
Author(s):  
Nasib Qureshi ◽  
Badal Saha ◽  
Siqing Liu ◽  
Rogers Harry‐O'kuru

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