scholarly journals Insecticidal activity of culture filtrates from liquid medium of Beauveria bassiana isolates from South Sumatra (Indonesia) wetland soil against larvae of Spodoptera litura

2019 ◽  
Vol 20 (8) ◽  
Author(s):  
DWI RIZKI AYUDYA ◽  
Siti Herlinda ◽  
SUWANDI SUWANDI
Keyword(s):  
Liquid Medium ◽  
Beauveria Bassiana ◽  
Culture Filtrate ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Wetland Soil ◽  
Liquid Media ◽  
Toxic Activity ◽  
Culture Filtrates ◽  
Beauveria Bassiana Isolates

Abstract. Ayudya DR, Herlinda S, Suwandi S. 2019. Insecticidal activity of culture filtrates from liquid medium of Beauveria bassiana isolates from South Sumatra (Indonesia) wetland soil against larvae of Spodoptera litura. Biodiversitas 20: 2101-2109. The obstacle in utilizing entomopathogenic fungi to control pest insects in wetlands is the inability of their isolates to survive during the saprophytic phase due to the soil being very acidic. Therefore, the exploration of fungi being able to survive in the acidic soil was utilized in the acidic ecosystem. This study aimed to test the toxic activity of Beauveria bassiana culture filtrates from liquid medium at pH 2.5 and 6 against Spodoptera litura larvae. Total of ten isolates collected from South Sumatra, Indonesia was grown in liquid media at pH of 2.5 and 6 for six weeks. The result showed that the culture filtrate from liquid medium at pH 6 was more toxic against the larvae than that at pH 2.5. The mortality of the larvae at pH 6 reached 92%, while that at pH 2.5 it reached 13.33% only. The findings of present study revealed that the culture filtrate isolates of BPdR, BJgTs, BSwTd2, BSwTd3, BSwTd4, BKKPp2 from pH 2.5 media still caused high larvae mortality (6.67-13.33%). These seven isolates are superior due to their toxic nature at pH 2.5. Consequently, present study has increased the chances of success in utilizing these isolates for biological control in acidic ecosystems, such as peatlands, tidal lowlands, and freshwater swamps.

scholarly journals Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of South Sumatra (Indonesia) against Spodoptera litura larvae

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
MIMMA GUSTIANINGTYAS ◽  
Siti Herlinda ◽  
SUWANDI ◽  
SUPARMAN ◽  
HARMAN HAMIDSON ◽  
...  
Keyword(s):  
Culture Filtrate ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Metarhizium Anisopliae ◽  
Spodoptera Litura ◽  
Fungal Culture ◽  
Fungal Metabolites ◽  
Liquid Media ◽  
Control Techniques ◽  
Toxic Culture

Abstract. Gustianingtyas M, Herlinda S, Suwandi, Suparman, Hamidson H, Hasbi, Setiawan A, Verawaty M, Elfita, Arsi. 2020. Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of South Sumatra against Spodoptera litura larvae. Biodiversitas 21: 1839-1849. The use of secondary fungal metabolites for the active ingredient of mycoinsecticide is more effective and more easily integrated with other pest control techniques. This study aimed to measure the toxicity of the culture filtrate of entomopathogenic fungi originating from South Sumatra against the Spodoptera litura larvae. Beauveria bassiana (25 isolates) and Metarhizium anisopliae (20 isolates) of South Sumatra were cultured in liquid media and filtered to produce culture filtrate. The larvae which were sick due to the filtrate showed the symptoms of decreased appetite and were not actively moving, while the dead larvae were characterized by being wrinkled, dry, black integument, and odorless. Mortality caused by B. bassiana filtrate was the highest 98% (BJgTs isolate) and not significantly different from the BSwTd2 isolate (94.67%). Yet, the LT50 BSwTd2 isolate was shorter (5.92 days) compared to the LT50 BJgTs isolate (6.35 days). The most toxic M. anisopliae filtrate produced the mortality of 96% (MKbTp2 isolate) and 85.33% (MPdB isolate) each of which had LT50 of 7.36 days and 8.09 days, respectively. So, the most toxic culture filtrate was BSwTd2 isolate of B. bassiana and MKbTp2 isolate of M. anisopliae. The entomopathogenic fungi producing filtrate which are toxic have the potential to be active ingredients of mycoinsecticides.

scholarly journals Toxic metabolites from culture filtrate of Fusarium oxysporum and its effects on cucumber cells and plantlets

1999 ◽  
Vol 30 (2) ◽  
pp. 104-106 ◽  
Author(s):  
Itamar Soares de Melo ◽  
Everaldo Piccinin
Keyword(s):  
Cell Death ◽  
Fusarium Oxysporum ◽  
Culture Filtrate ◽  
Single Cells ◽  
Fluorescein Diacetate ◽  
Toxic Activity ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
High Level

Resistance of cucumber plantlets to culture filtrate of Fusarium oxysporum is correlated with resistance of single cells from callus. Single cells and plantlets of two cultivars of cucumber were incubated with culture filtrates. Rapid cell death occurred, as assessed by the stain fluorescein diacetate. More cell death ocurred in the cells of the cultivar Aodai than in to cells of the cultivar Caipira, which presented high level of resistance. Maximum toxic activity of culture filtrates was attained after 21-25 days of growth of the fungus.

scholarly journals New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Siti Herlinda ◽  
Riski Anwar Efendi ◽  
Radix Suharjo ◽  
Hasbi Hasbi ◽  
Arum Setiawan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Culture Filtrate ◽  
Entomopathogenic Fungi ◽  
Insecticidal Activity ◽  
Metarhizium Anisopliae ◽  
Internal Transcribed Spacer ◽  
Spodoptera Litura ◽  
Its Region ◽  
Morphological Characters ◽  
Penicillium Citrinum

Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.

scholarly journals The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

2007 ◽  
Vol 12 (2) ◽  
Author(s):  
M. Estrada ◽  
Manuel Camacho ◽  
César Benito
Keyword(s):  
Beauveria Bassiana ◽  
The Other ◽  
Caribbean Region ◽  
Pcr Markers ◽  
Issr Primer ◽  
Geographic Origins ◽  
The Caribbean ◽  
Beauveria Bassiana Isolates ◽  
High Level ◽  
Issr Pcr

AbstractInter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

scholarly journals AKTIVITAS EKSTRAK BIJI TANAMAN MINDI MELIA AZEDARACH (L.) TERHADAP SPODOPTERA LITURA (F.) (LEPIDOPTERA: NOCTUIDAE)

2005 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Hamdani Hamdani
Keyword(s):  
Biological Activity ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Developmental Time ◽  
Seed Extract ◽  
Melia Azedarach ◽  
The Third ◽  
First Instar ◽  
Lepidoptera Noctuidae

Activity of Melia azedarach (L.) seed extract against armyworm Spodoptera litura (F.) (Lepidoptera: Noctuidae). This study was conducted to evaluate the effectivenes and biological activity of Melia azedarach L. (Meliaceae) seed extract against armyworm, Spodoptera litura F. The first instar larvae were fed extract-treated cotton leaves for 2 days, then were maintained on untreated leaves until the third instar stage. Records were kept in regard to the larvae mortality and developmental time of surviving larvae from first instar to third instar. The result showed that Melia azedarach L. seed extract at consentration of 50 g of seeds/l of water (5%) exhibited moderate insecticidal activity against S. litura larvae (43.33 - 68.33% mortality). Addition of detergen at 0.2% to extract did not increase insecticidal activity of the extract. However, boiling seed extract at consentration of 50 g of seeds/l of water (5%) during 10 until 20 minutes increased insecticidal activity of extract (66.67 - 68.33% mortality). Generally, M. azedarach seed extract treatment did not affect  developmental time of  S. litura larvae.

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