scholarly journals Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Ima Fauziah ◽  
Widya Asmara ◽  
Agnesia Endang Tri Hastuti Wahyuni ◽  
Rini Widayanti
Keyword(s):  
Short Communication ◽  
Pcr Detection ◽  
Molecular Techniques ◽  
Specific Pcr ◽  
Infectious Coryza ◽  
Avibacterium Paragallinarum ◽  
Poultry Farms ◽  
Commercial Poultry ◽  
Tract Disease ◽  
Species Specific

Abstract. Fauziah I, Asmara W, Wahyuni AETH, Widayanti R. 2021. Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia. Biodiversitas 22: 4890-4894. Infectious coryza (IC), caused by Avibacterium paragallinarum, is a contagious and infectious respiratory tract disease that affects the commercial poultry industry. Molecular techniques, such as species-specific PCR, HPG-2 PCR are mostly used for the detection of  A. paragallinarum. The current research was carried out to isolate A. paragallinarum from the layers of infectious coryza signs in Sleman District, special region of Yogyakarta, followed by PCR confirmation of the identified bacteria. Nine field isolates were observed and determined based on their colony and cell morphology. All isolates were characterized biochemically and confirmed with species-specific HPG-2 PCR for A. paragallinarum. Out of 9 isolates, 6 (66.7%) isolates were biochemically identified as A. paragallinarum and confirmed by HPG-2 PCR.

Antimicrobial susceptibility of Avibacterium paragallinarum isolates from outbreaks of infectious coryza in Dutch commercial poultry flocks, 2008–2017

2018 ◽  
Vol 217 ◽  
pp. 135-143 ◽  
Author(s):  
Annet Heuvelink ◽  
Jeanine Wiegel ◽  
Corinna Kehrenberg ◽  
Remco Dijkman ◽  
Edgardo Soriano-Vargas ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Infectious Coryza ◽  
Avibacterium Paragallinarum ◽  
Commercial Poultry

Species specific PCR detection protocol for the main mycotoxin-producing Aspergillus species in paprika

2010 ◽  
Author(s):  
N. Sardiñas Díaz ◽  
J. Gil-Serna ◽  
B. Patiño Álvarez ◽  
M.T. González-Jaén ◽  
C. Vázquez Estévez
Keyword(s):  
Pcr Detection ◽  
Aspergillus Species ◽  
Specific Pcr ◽  
Species Specific

scholarly journals Survey of Vaginal-Flora Candida Species Isolates from Women of Different Age Groups by Use of Species-Specific PCR Detection

2008 ◽  
Vol 46 (4) ◽  
pp. 1501-1503 ◽  
Author(s):  
J.-P. Vermitsky ◽  
M. J. Self ◽  
S. G. Chadwick ◽  
J. P. Trama ◽  
M. E. Adelson ◽  
...  
Keyword(s):  
Candida Species ◽  
Age Groups ◽  
Vaginal Flora ◽  
Pcr Detection ◽  
Specific Pcr ◽  
Species Specific

Molecular identification of zoonotic hookworm species in dog faeces from Tanzania

2018 ◽  
Vol 93 (3) ◽  
pp. 313-318 ◽  
Author(s):  
A. Merino-Tejedor ◽  
P. Nejsum ◽  
E.M. Mkupasi ◽  
M.V. Johansen ◽  
Annette Olsen
Keyword(s):  
Sanger Sequencing ◽  
Molecular Techniques ◽  
Health Concern ◽  
Specific Pcr ◽  
Molecular Approaches ◽  
Faecal Samples ◽  
Pcr Rflp ◽  
Veterinary Importance ◽  
Polymerase Chain ◽  
Species Specific

AbstractThe presence and distribution of various species of canine hookworms in Africa are poorly known. The main objective of this study, therefore, was to identify the hookworm species present in canine faecal samples from Morogoro, Tanzania, using molecular techniques. Faecal samples from 160 local dogs were collected and hookworm positive samples processed to recover larvae for further molecular characterization. DNA was extracted from pools of larvae from individual samples (n = 66), which were analysed subsequently using two different molecular approaches, polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) and species-specific PCR coupled with Sanger sequencing. The PCR-RFLP technique detected only the presence of the ubiquitousAncylostoma caninumin the 66 samples. However, by species-specific PCR coupled with Sanger sequencing we identified ten samples withA. braziliense, two withUncinaria stenocephalaand five withA. ceylanicum. Thus, all four known species of canine hookworms were identified in Morogoro, Tanzania. To our knowledge this is the first report of the detection of the presence ofU. stenocephalaandA. ceylanicumin Africa using molecular techniques. In addition to their veterinary importance, canine hookworms have zoonotic potential and are of public health concern.

Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

Nematology ◽  
2009 ◽  
Vol 11 (3) ◽  
pp. 471-480 ◽  
Author(s):  
Robert Robbins ◽  
Allen Szalanski ◽  
Chang-Hwan Bae
Keyword(s):  
Multiplex Pcr ◽  
Dna Sequences ◽  
Rflp Analysis ◽  
Pcr Primers ◽  
Interspecific Variation ◽  
Molecular Techniques ◽  
Specific Pcr ◽  
Pcr Multiplex ◽  
Pcr Rflp ◽  
Species Specific

AbstractTwo different molecular approaches, a multiplex PCR and PCR-RFLP of ITS-rDNA, were developed for the identification of Hoplolaimus species. DNA sequences of H. columbus, H. galeatus, H. concaudajuvencus, H. magnistylus, H. seinhorsti and three undescribed Hoplolaimus species were used to design species-specific primers. Three reverse species-specific PCR primers for H. columbus, H. galeatus and H. magnistylus were developed using the ITS1 region exhibiting interspecific variation. Three species-specific PCR primers in combination with the forward primer, Hoc-1f, produced distinct amplicons of 580 bp for H. columbus, 120 bp for H. galeatus and 340 bp for H. magnistylus. We successfully identified each of three species by multiplex PCR when all three were mixed in a single PCR reaction. Restriction enzyme digests of the PCR amplicon using HaeIII and RsaI permitted discrimination of H. columbus, H. galeatus, H. magnistylus, H. concaudajuvencus, H. sp. 1, H. sp. 2 and H. sp. 3 from each other. These results suggest that these molecular techniques allow for rapid, easy and reliable identification of Hoplolaimus species.

scholarly journals Species-specific PCR detection of the fish pathogen, Vibrio anguillarum, using the amiB gene, which encodes N-acetylmuramoyl-l-alanine amidase

2007 ◽  
Vol 269 (2) ◽  
pp. 201-206 ◽  
Author(s):  
Gyeong-Eun Hong ◽  
Dong-Gyun Kim ◽  
Ju-Yoon Bae ◽  
Sun-Hee Ahn ◽  
Sungchul C. Bai ◽  
...  
Keyword(s):  
Vibrio Anguillarum ◽  
Pcr Detection ◽  
Fish Pathogen ◽  
Specific Pcr ◽  
Species Specific

scholarly journals Incidence, Serotyping and Antimicrobial Susceptibility Profile of Avibacterium Paragallinarum Isolated From Local Commercial Poultry of Balochistan

2020 ◽  
Author(s):  
Ghulam Muhammad ◽  
muhammad kamran Taj ◽  
Imran Taj ◽  
Iqbal Panezai ◽  
Ferhat Abbas ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Optimal Growth ◽  
Nasal Discharge ◽  
Pathogenicity Test ◽  
Upper Respiratory Tract ◽  
Infectious Coryza ◽  
Avibacterium Paragallinarum ◽  
Facial Swelling ◽  
Serotype B ◽  
Tract Disease

Abstract Background Infectious coryza (IC) caused by Avibacterium paragallinarum, a Gram negative, non-motile coccobacilli, is a severe upper respiratory tract disease of poultry. This study aimed to report on the isolation rate and serotyping of Avibacterium paragallinarum causing Infectious Coryza (IC) in commercial layer poultry in Balochistan. Results Total 500 samples were collected from IC-suspected or recently dead birds Results revealed that 80.62% of sample were found positive for A. paragallinarum. Of these, serotype B was 59.60% and serotype C was 21.02%. The isolates of A. paragallinarum were growing well at 35–37 oC, however, growth rate was declined at 24 oC, and 42 oC. Similarly, A. paragallinarum showed optimal growth between pH 5 and 9, but the superlative pH growth values were from 6 to 8 pH. Antimicrobial susceptibility test showed that all tested isolates displayed resistance against metronidazole, colistin sulphate, bacitracin, streptomycin, chloramphenicol and lincomycine, while were found susceptible to tetracycline, erythromycin, vancomycin, amoxicillin, and ciprofloxacin. Pathogenicity test performed indicated that experimental birds showed signs of dullness, fever, serous nasal discharge and facial swelling with pus inside infra-orbital sinus, severe congestion in the trachea and partial cloudiness of air sacs. conclusions Overall, we report, for the first time, on high incidence rate of pathogenic A. paragallinarum among layer birds.

Development of a species-specific PCR to detect the cereal cyst nematode, Heterodera latipons

Nematology ◽  
2013 ◽  
Vol 15 (6) ◽  
pp. 709-717 ◽  
Author(s):  
Fateh Toumi ◽  
Fateh Toumi ◽  
Lieven Waeyenberge ◽  
Fateh Toumi ◽  
Lieven Waeyenberge ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Actin Gene ◽  
Specific Primers ◽  
Second Stage ◽  
Specific Pcr ◽  
Pcr Products ◽  
Heterodera Latipons ◽  
Species Specific ◽  
Very High

Several Heterodera species can reduce the yield of wheat and barley, among which H. avenae, H. filipjevi and H. latipons are economically the most important. Their identification, based on morphological characteristics, is not straightforward but can be made easier using molecular techniques. In this study, we developed species-specific primers for the detection of H. latipons. The actin gene of eight Heterodera species was partially sequenced and, after purifying and sequencing the PCR products, all sequences were aligned to find unique sites. The alignment showed moderate to very high similarities between the species. However, a small fragment of the actin gene was suitable for the construction of a potentially useful species-specific primer for H. latipons. The optimised PCR was subsequently tested with several populations of 14 Heterodera species and a single population of Punctodera punctata. Heterodera latipons was represented by 16 populations originating from six different countries. The primer set (Hlat-act), designed using AlleleID 7.73, was shown to be very specific. To test its sensitivity further, the PCR was conducted on DNA extracted from five second-stage juveniles (J2) of H. latipons mixed with five or 100 J2 belonging to H. avenae. The PCR was able to detect up to 1:10 dilution of the DNA obtained from five J2. The results showed that a specific and sensitive H. latipons species-specific PCR was constructed.

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