scholarly journals Effects of Hibiscus sabdariffa Linn. on insulin-like growth factor binding protein 3 (IGFBP-3) to prevent overtraining syndrome

2015 ◽  
Vol 23 (4) ◽  
pp. 187-91 ◽  
Author(s):  
Ermita I.I. Ilyas ◽  
Neng T. Kartinah ◽  
Trinovita Andraini ◽  
Roman A. Goenarjo ◽  
Donna N. Kahandjak
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Male Rats ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Overtraining Syndrome ◽  
Significant Difference ◽  
Igfbp 3

Background: Excessive physical exercises (overtraining) can increase the production of reactive oxygen species (ROS). One of the indicators of overtraining syndrome is a decrease in insulin-like growth factor binding protein 3 (IGFBP-3). Administration of Hibiscus sabdariffa Linn., a powerful antioxidant, is expected to boost endogenous antioxidants, and thus prevents overtraining. The aim of this study is to determine the effect of H. sabdariffa on IGFBP-3 levels in rats under ”overtraining physical excersice”.Methods: This experimental study was conducted on 30 male rats (Rattus norvegicus 200-250 grams), randomly allocated into 5 groups: 1) control group (C); 2) control with H. sabdariffa (C-Hib); 3) mild aerobic exercise (A-Ex); 4) overtraining exercise (OT); 5) overtraining exercise with H. Sabdariffa (OT-Hib). H. sabdariffa (400 mg/kg/d, 11 weeks) were administered orally via syringe cannula. IGFBP-3 was measured by using ELISA (Cusa bio kit) and data were analyzed with ANOVA test.Results: Plasma level of IGFBP-3 in the C and OT groups were 17.4 ± 10 mIU/L, the lowest in OT groups (10.7 ± 9.9 mIU/L) and the OT-Hib group had the highest level (31.5 ± 6.2 mIU/L). There was significant difference of the level IGFBP-3 in OT groups with A-Ex groups (10.7 ± 9.9 vs 23.5 ± 9.7 mIU/L; p < 0,05). The significant difference was also observed in the level of IGFBP 3 between C groups and the OT-Hib groups (17.4 ± 10 vs 31.5 ± 6.2; p < 0.05).Conclusion: Administration of H. sabdariffa can prevent the decrease of IGFBP-3 levels in overtraining rats, indicating its role in preventing overtraining syndrome.

scholarly journals Malnutrition in children associated with low insulin-like growth factor binding protein-3 (IGFBP-3) levels

2021 ◽  
Vol 35 ◽  
pp. S275-S277
Author(s):  
Dwi Ayu Rahmawati ◽  
Aminuddin Aminuddin ◽  
Firdaus Hamid ◽  
Prihantono Prihantono ◽  
Burhanuddin Bahar ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Igfbp 3

scholarly journals Transcriptional and Posttranscriptional Regulation of Insulin-Like Growth Factor Binding Protein-3 by Cyclic Adenosine 3′,5′-Monophosphate: Messenger RNA Stabilization Is Accompanied by Decreased Binding of a 42-kDa Protein to a Uridine-Rich Domain in the 3′-Untranslated Region

1999 ◽  
Vol 13 (3) ◽  
pp. 495-504
Author(s):  
N. E. Erondu ◽  
J. Nwankwo ◽  
Y. Zhong ◽  
M. Boes ◽  
B. Dake ◽  
...  
Keyword(s):  
Growth Factor ◽  
Untranslated Region ◽  
Specific Binding ◽  
Binding Protein ◽  
Binding Activity ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Mdbk Cells ◽  
Camp Induction ◽  
Igfbp 3

Abstract The Madin Darby bovine kidney (MDBK) cell line was used to investigate the mechanisms underlying the cAMP regulation of insulin-like growth factor binding protein-3 (IGFBP-3) gene expression. Treatment of confluent monolayers either with forskolin or cAMP produced a 60- to 75-fold induction of IGFBP-3 mRNA and protein levels. This effect did not require new protein synthesis as inhibition of translation by cycloheximide actually caused a 2-fold increase in the cAMP induction. The rates of IGFBP-3 gene transcription, assessed by nuclear run-on assays, increased approximately 15-fold in cells exposed to cAMP. In addition, the half-life of the IGFBP-3 mRNA transcript was increased ∼3-fold in the presence of cAMP. Gel mobility shift and competition experiments revealed the specific binding of an approximately 42-kDa cytoplasmic protein factor to the 3′-untranslated region (3′-UTR) of the IGFBP-3 mRNA. A 21-nucleotide uridine-rich segment that contained no AUUUA motif was sufficient for the specific binding. The binding activity of this protein was reduced after cAMP treatment but was increased by phosphatase treatment. In conclusion, the cAMP induction of IGFBP-3 mRNA in MDBK cells occurred at both the transcriptional and posttranscriptional levels. The IGFBP-3 mRNA stabilization in MDBK cells probably involved the phosphorylation of a member of the family of U-rich region mRNA-binding proteins and is the first reported member whose RNA-binding activity is reduced by cAMP.

Oestrogen regulation of insulin-like growth factor binding protein-3 (IGFBP-3) and expression of IGFBP-3 messenger RNA in the ovine endometrium

1998 ◽  
Vol 10 (3) ◽  
pp. 241 ◽  
Author(s):  
A. J. Peterson ◽  
A. M. Ledgard ◽  
S. C. Hodgkinson
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Oestrous Cycle ◽  
Endometrial Tissue ◽  
Northern Analysis ◽  
Northern Blot Hybridization ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Oestradiol Treatment ◽  
Igfbp 3

Transcriptional regulation of insulin-like growth factor binding protein-3 (IGFBP-3) by oestrogen was investigated by Northern blot hybridization of endometrial tissue mRNA from anoestrous ewes treated with oestradiol-17β at either 0, 40 or 80 µg per day for 7 days. The 2.6 kb IGFBP-3 transcript, seen in control animals, was virtually undetectable in treated animals. This suppressive effect was reflected in Western ligand blots of the uterine luminal fluid (ULF) proteins where the concentration of IGFBP-3 was significantly decreased with increasing oestrogen treatments. IGFBP-2 levels were increased with oestradiol treatment but no significant effect was seen on the other minor IGFBP’s present in the ULF. Northern analysis also showed that the IGFBP-3 transcript was present from days 12 to 16 of the oestrous cycle but was either absent or very weak on days 0 (oestrus) and 9 of cycling ewes. In situ hybridization of endometrial tissue sections localized the IGFBP-3 mRNA to the luminal epithelial cell layer, areas of the stromal tissue and in some glandular epithelial cells. Oestradiol treatment of ewes down-regulated expression of IGFBP-3 in the endometrium; therefore, the low levels of IGFBP-3 in ULF during the early part of the oestrous cycle is possibly due to elevated levels of plasma oestradiol around oestrus.

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