Toxicological Effects of Aqueous Leaf Extract of Bitter (Vernonia Amygdalina) on Liver Enzymes of Albino Rats

2021 ◽  
Vol 8 (1) ◽  
Keyword(s):  
Leaf Extract ◽  
Liver Enzymes ◽  
Albino Rats ◽  
Vernonia Amygdalina ◽  
Toxicological Effects ◽  
Aqueous Leaf Extract

scholarly journals Histopathological effect of aqueous bitter leaf extract (Vernonia amygdalina) on acetaminophen-induced liver damage in albino rat (Rattus norvegicus)

2020 ◽  
Vol 47 (4) ◽  
pp. 24-31
Author(s):  
C. O. Raimi ◽  
W. A. Oyelade ◽  
A. O. Iyanda
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Albino Rats ◽  
Vernonia Amygdalina ◽  
Leaf Extracts ◽  
Aqueous Leaf Extract ◽  
Group 2

Liver diseases are a worldwide problem. Medicinal plants are being increasingly utilized to treat a wide variety of diseases, liver disorder inclusive. The effect of aqueous leaf extracts of Vernonia amygdalina on acetaminophen-induced liver damage via the assessment of the histopathological examination was examined. Five groups of albino rats were used (n = 5). Animals in group 1 were fed normal laboratory pellet and water ad libitum (Control); those in group 2 received 3000mg/kg of acetaminophen alone. Groups 3, 4 and 5 received 3000mg/kg of acetaminophen and in addition 50mg/kg Vernonia amygdalina leaf extract. 100mg/kg Vernonia amygdalina leaf extract and 200mg/kg Silymarin, respectively for 14 days. Twenty-four hours after the last administration, the animals were sacrificed. The organs (liver) was excised and used for the histopathological examination. Histological examinations of the liver showed acetaminophen-induced hepatotoxicity. Histology of the liver revealed altered cellular architecture (poor architecture, moderate to severe congestion of the portal vein as well as severe peri portal infiltration of inflammatory cells, the liver parenchyma also showed area with destroyed liver plates with severe hemorrhage and necrosis. The morphology of the hepatocytes showed some degenerated liver cells, the liver parenchyma also showed focal area of moderate aggregate of inflammatory cells) following administration of acetaminophen (Group 2). Normal histological features were restored after treatment with aqueous leaf extract of Vernonia amygdalina. The study has demonstrated that aqueous leaf extract of Vernonia amygdalina ameliorate liver of rats against acetaminophen-induced toxicity and modulates the adverse effects of acetaminophen on the liver

scholarly journals Antidiabetic activity and acute toxicity evaluation of aqueous leaf extract of Vernonia amygdalina

2014 ◽  
Vol 13 (50) ◽  
pp. 4586-4593 ◽  
Author(s):  
A. Momoh M. ◽  
O. Adedokun M. ◽  
T. Mora A. ◽  
A. Agboke A.
Keyword(s):  
Acute Toxicity ◽  
Leaf Extract ◽  
Antidiabetic Activity ◽  
Vernonia Amygdalina ◽  
Toxicity Evaluation ◽  
Aqueous Leaf Extract

scholarly journals Antioxidant and Hepatoprotective Potentials of Lasimorpha senegalensis Schott Leaf Extract on Carbon Tetrachloride-induced Liver Damage in Rats

2020 ◽  
pp. 70-78
Author(s):  
Chinyere Blessing Chigor ◽  
Felix Ifeanyi Nwafor ◽  
Edith Ugwuja ◽  
Chisimdi S. Obi
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Liver Enzymes ◽  
Radical Scavenging Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Albino Rats ◽  
Endogenous Antioxidants ◽  
Wistar Albino Rats

Aims: The present study assessed the antioxidant and hepatoprotective potentials of the methanolic leaf extract of Lasimorpha senegalensis – a medicinal plant used by the indigenous people of Nigeria to treat hepatitis and feverish conditions. Place and Duration of Study: The research work was conducted in the Department of Pharmacognosy and Environmental Medicine and Department of Plant Science and Biotechnology, both in the University of Nigeria, Nsukka, from May to August, 2019. Methodology: Phytochemical analyses and acute toxicity study of the sample followed standard procedures. In vitro antioxidant assay was by DPPH and H2O2 models. A total of 25 male Wistar albino rats (120 – 150 g) were grouped into five, each group with five animals. Hepatotoxicity was induced with carbontetrachloride (1 ml/kg). The treatment groups (3-5) received extract (200 and 400 mg/kg) and Silymarin (100 mg/kg). Endogenous antioxidants (superoxide dismutase, catalase, glutathione peroxidase), plasma malondialdehyde and liver enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphate) were determined after treatment. Results: The results showed the leaf extract had appreciable amounts of bioactive phytochemicals and free radical scavenging activity (IC50 of 0.52 mg/ml and 0.71 mg/ml for DPPH and H2O2 respectively) with no toxicity at 5000 mg/kg. The extract also elevated the endogenous antioxidants and significantly (p ≤ .05) reduced lipid peroxidase and liver enzymes. Conclusion: This report justifies the local use of this plant in the management of various diseases related to oxidative stress and liver damage.

scholarly journals In vitro Antioxidant and In vivo Hepatocurative and Nephrocurative Activities of Aqueous Leaf Extract of Newbouldia laevis in Albino Rats

2021 ◽  
pp. 114-125
Author(s):  
Mohammed A. Sulaiman ◽  
Mahmoud S. Jada ◽  
Augustine Elizabeth ◽  
Abubakar Umar Modibbo
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Kidney Diseases ◽  
Albino Rats ◽  
Protein Levels ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Aqueous Leaf Extract

The in vitro antioxidant activity and in vivo hepatocurative and nephrocurative potential of Newbouldia laevis aqueous leaf extract (NLALE) was evaluated. The study used 30 male, albino rats (Rattus norvegicus) weighing 180 ± 20 g, of which 25 were intoxicated by oral administration of a single dose of diclofenac (100 mg/kg b. wt.). Animals were treated by oral administration of silymarin (200 mg/kg b. wt.), furosemide (1.5 mg/kg b. wt.) and NLALE (200 mg/kg and 400 mg/kg b. wt.) for seven consecutive days before animals were sacrificed on the 8th day and serum/plasma was analyzed for biochemical markers of hepatotoxicity and nephrotoxicity. Phytochemical screening of NLALE revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids and tannins. The extract scavenged DPPH radical, reduced Fe3+ and inhibited TBARs in comparable manner to ascorbic acid in vitro. NLALE also attenuated diclofenac-induced liver and kidney intoxication as indicated by the significantly (p<0.05) reduced levels of serum biomarkers of hepatotoxicity: ALT, AST, bilirubin, but increased total protein levels and nephrotoxicity: urea, creatinine, Na+ and K+. The observed effects are dose dependent as the 400 mg/kg b. wt. appeared to be more potent than the 200 mg/kg b. wt. dose. It may be concluded from this study that Newbouldia laevis leaf has ameliorative effect against diclofenac-induced hepatotoxicity and nephrotoxicity probably through antioxidative mechanism and the curative claim and the folkloric use of the plant in the treatment of liver and kidney diseases have been scientifically validated

scholarly journals HEPATOPROTECTIVE AND ANTI-HEPATOTOXIC ACTIVITIES OF AQUEOUS LEAF EXTRACT OF TACAZZEA BARTERI AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ALBINO RATS

2013 ◽  
Vol 2 (9) ◽  
pp. 60-65 ◽  
Author(s):  
Obioha Mary Quinette Uru ◽  
Ilodigwe Emmanuel Emeka ◽  
Ajaghaku Daniel Lotanna ◽  
Umeokoli Blessing Ogechukwu
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Albino Rats ◽  
Aqueous Leaf Extract
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