scholarly journals Immunohistochemical Evaluation of a Malignant Intestinal Carcinoid in a Dog

2003 ◽  
Vol 40 (2) ◽  
pp. 212-215 ◽  
Author(s):  
T. Sako ◽  
E. Uchida ◽  
M. Okamoto ◽  
E. Yamamoto ◽  
Y. Kagawa ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Neuron Specific Enolase ◽  
Clinical History ◽  
Intestinal Bleeding ◽  
Neoplastic Cells ◽  
Protein Gene Product ◽  
Eosinophilic Cytoplasm ◽  
Multiple Metastases ◽  
History Of ◽  
Cytokeratin 13

An intestinal carcinoid with multiple metastases was identified in a 5-year-old male Shih Tzu with a clinical history of anemia, fatigue, anorexia, vomiting, intermittent diarrhea, intestinal bleeding, and progressive emaciation. There was a yellowish-white mass 15 mm in diameter in the anterior jejunum and white nodules consistent with metastases in many organs. Histopathologically, the mass consisted of neoplastic cells arranged in lobules, trabeculae, or closely interdigitating islands of cells. Neoplastic cells were generally polygonal with round hyperchromatic nuclei, modest amounts of eosinophilic cytoplasm, and eosinophilic cytoplasmic granules. Mitoses were common. Rosette formations of tumor cells were apparent in metastatic tumors. Immunohistochemically, tumor cells stained positive for cytokeratin 13, synaptophysin, protein gene product 9.5, neuron-specific enolase, chromogranin A, calcitonin gene-related peptide, serotonin (5-HT), and Leu-7. Serum 5-HT concentrations for this dog were increased 10-fold compared with those of normal dogs. All findings were consistent with a diagnosis of a malignant intestinal carcinoid.

Cardiomyopathy Associated with Angiomatous Pheochromocytoma in a Rhesus Macaque (Macaca mulatta)

2003 ◽  
Vol 40 (4) ◽  
pp. 468-473 ◽  
Author(s):  
P. Vogel ◽  
D. Fritz
Keyword(s):  
Rhesus Macaque ◽  
Tumor Cells ◽  
Macaca Mulatta ◽  
Interstitial Fibrosis ◽  
Neuron Specific Enolase ◽  
Clinical History ◽  
Chronic Heart Disease ◽  
S 100 ◽  
Prolonged Recovery ◽  
History Of

A 24-year-old female rhesus macaque ( Macaca mulatta) presented with a clinical history of chronic heart disease and prolonged recovery from sedation and anesthesia. At necropsy, the heart was markedly enlarged, with thinned ventricular walls, dilated chambers, and severe left atrioventricular valvular insufficiency. The ventricular walls contained numerous narrow, pale, often coalescing streaks that often extended along vessels into the deeper myocardium. Histologically, bands of interstitial fibrosis in the heart were associated with areas of myofiber atrophy, myofibril degeneration and loss, and inflammation. The left adrenal gland contained a 2 × 1 × 0.5 cm tumor with an unusual angiomatous pattern consisting largely of blood-filled sinusoids lined by one to four layers of low cuboidal to polyhedral tumor cells. In most sinusoids, the tumor cells appeared to be in direct contact with blood, although endothelial cells lined some sinusoids. Ultrastructurally, the tumor cells contained abundant electron-dense cytoplasmic granules. These granules were argyrophilic by Sevier-Munger staining and intensely immunoreactive for synaptophysin, chromogranin A, neuron-specific enolase, and S-100. These findings helped confirm the diagnosis of angiomatous pheochromocytoma. The heart lesions match those reported for catecholamine cardiomyopathy in other species.

Malignant Melanoma Presenting as an Intrathymic Tumor

2000 ◽  
Vol 124 (1) ◽  
pp. 130-134
Author(s):  
P. M. Alli ◽  
B. J. Crain ◽  
R. Heitmiller ◽  
P. Argani
Keyword(s):  
Malignant Melanoma ◽  
Previous History ◽  
Clinical History ◽  
Computed Tomographic ◽  
Eosinophilic Cytoplasm ◽  
S 100 ◽  
History Of ◽  
Solitary Metastases ◽  
Physical Findings ◽  
Pleomorphic Cells

Abstract The identification of malignant melanoma in a visceral organ of nonepidermal origin is not an uncommon occurrence. Frequently, these cases are solitary metastases that present years after a thin epidermal melanoma has been diagnosed (and sometimes forgotten). However, primary visceral melanomas have been reported that have not been preceded by an epidermal lesion. We describe herein a unique case of melanoma presenting as a primary intrathymic tumor. The patient had no previous history of epidermal melanoma, and extensive workup did not reveal evidence for an alternative primary site. The tumor exhibited histologic features characteristic of melanoma, including an abundance of large pleomorphic cells with eosinophilic cytoplasm, prominent nucleoli, and occasional intranuclear inclusions. Tumor cells stained for HMB-45 and S-100 protein and ultrastructural analysis revealed stage II and stage III melanosomes. The patient remained free of disease until intrathoracic recurrence was detected on a computed tomographic scan 14 months later. The lack of clinical history and physical findings of melanoma at presentation, the intrathymic location of the tumor, and the pattern of recurrence suggest that this case likely represents a primary thymic melanoma, a previously unreported entity.

scholarly journals Primary intestinal fibrosarcoma caused by intestinal perforation in a dog: a case report

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 314-319 ◽  
Author(s):  
H. Avci ◽  
R. Yaygingul ◽  
M. Gultekin ◽  
ET Epikmen ◽  
K. Ural ◽  
...  
Keyword(s):  
Intestinal Perforation ◽  
Present Report ◽  
Abdominal Mass ◽  
Neuron Specific Enolase ◽  
Intestinal Wall ◽  
Tumour Mass ◽  
Neoplastic Cells ◽  
S 100 ◽  
History Of ◽  
Gross Examination

An 11-year-old male Cocker Spaniel was examined for a palpable abdominal mass located in the jejunum after presenting with a history of anorexia and constipation for several weeks. In a contrast radiogram, a structure with well-defined borders adjacent to the intestine was determined. The intestinal mass, measuring 16 × 9.19 × 8.6 cm and weighing 900 g was surgically removed. At gross examination, when the lumen of a portion of the intestine excised together with the tumour mass was exposed, an ulcerated, oval-shaped area 1.2 × 0.6 cm in size was observed on the mucosa. The outer surface of the tumour was homogenous and expanded outwards from the intestinal wall. Histologically, the tumour was composed of fusiform-elongated spindle-shaped to polygonal neoplastic cells forming interlacing fascicles or interwoven bundles in an atypical herringbone pattern. Immnunohistochemically, neoplastic cells stained intensely positive for vimentin, and negative for α-SMA, desmin, cyotokeratin (AE1/AE3), S-100 protein, glial fibrillary acidic protein, neuron specific enolase and synaptophsin. On the basis of the histopathological and immunohistochemical results, the tumour was diagnosed as a fibrosarcoma. The present report is a very rare description of fibrosarcoma of the dog intestine associated with intestinal perforation.  

scholarly journals Ovarian female adnexal tumor of probable Wolffian origin – Case report

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 899-903
Author(s):  
Ljiljana Vučković ◽  
Aleksandra Klisic ◽  
Mirjana Miladinović
Keyword(s):  
Case Report ◽  
Tumor Cells ◽  
Epithelial Membrane Antigen ◽  
Neuron Specific Enolase ◽  
Diagnostic Algorithm ◽  
Accurate Diagnosis ◽  
Histopathological Analysis ◽  
Eosinophilic Cytoplasm ◽  
Adnexal Tumor ◽  
Adnexal Tumors

Abstract Background During embryonic development in women, a regression of temporary embryonic structures – mesonephric (Wolffian) ducts occurs. Adnexal tumors of Wolffian duct origin (FATWO) are rare. Case report We presented the case of a 64-year-old female patient who was diagnosed with FATWO. After the surgical treatment, the uterus with bilateral adnexal structures was submitted for histopathological analysis. The left ovary was occupied by a tumor measuring 80 × 60 × 50 mm, with smooth, shiny, whitish surface. Tumor cells were medium-sized, relatively uniform, round, and polygonal, with eosinophilic cytoplasm and centrally laid nucleus with fine chromatin, organized into solid, trabecular, and tubular formations. Tumor cells were positive for pancytokeratin (CK), CK7, CD10, neuron-specific enolase (NSE), synaptophysin, calretinin, progesterone, estrogen, and epithelial membrane antigen (EMA). Conclusion This case adds a report of a rare tumor to the literature. We must think of it in the differential diagnostic algorithm to make an accurate diagnosis for selecting the best treatment modality.

scholarly journals Sporadic hemangioblastoma of the kidney: a clinicopathologic study of three cases and a literature review

2021 ◽  
Vol 49 (7) ◽  
pp. 030006052110277
Author(s):  
Yanmei Xu ◽  
Xuehua Ma ◽  
Yong Ma ◽  
Juan Li ◽  
Renya Zhang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
S100 Protein ◽  
Neuron Specific Enolase ◽  
Renal Tumors ◽  
Vascular Networks ◽  
Uniform Size ◽  
Clinicopathologic Study ◽  
Eosinophilic Cytoplasm ◽  
Lipid Vacuoles ◽  
Cluster Of Differentiation

Much attention has been paid to renal hemangioblastoma, but there are still challenges in its differential diagnosis. Three cases (2 men, 1 woman; age: 40–56 years) presented with renal tumors. The tumors were surrounded by a thick fibrous capsule, well-demarcated from the surrounding renal parenchyma, and composed of sheets or nests of polygonal to short spindle-shaped tumor cells with a rich capillary network. In cases 1 and 3, the large polygonal tumor cells contained abundant pale or eosinophilic cytoplasm, and some possessed intracytoplasmic lipid vacuoles. In case 2, tumor cells were characterized by a uniform size, mild, clear, or lightly stained cytoplasm, and typical "clear cell" appearance. Immunohistochemistry revealed that the polygonal stromal cells were strongly and diffusely positive for α-inhibin, neuron-specific enolase (NSE), S100 protein, and vimentin. Cluster of differentiation (CD)10 and paired box gene (PAX)8 were positive, while epithelial membrane antigen (EMA) and cytokeratin (CK) were focally positive in case 3. CD34 and CD31 outlined the contours and distribution of the vascular networks. Renal hemangioblastoma is rare and prone to misdiagnosis; more attention should be paid to the morphological features and reasonable application of immunohistochemistry in the diagnosis of hemangioblastoma.

scholarly journals A Subependymal Giant Cell Astrocytoma in a Cat

2000 ◽  
Vol 37 (3) ◽  
pp. 275-278 ◽  
Author(s):  
S. Duniho ◽  
F. Y. Schulman ◽  
A. Morrison ◽  
H. Mena ◽  
A. Koestner
Keyword(s):  
Giant Cell ◽  
Neuron Specific Enolase ◽  
Subependymal Giant Cell Astrocytoma ◽  
Neurofilament Protein ◽  
Neoplastic Cells ◽  
S 100 ◽  
History Of ◽  
The Right ◽  
Pleomorphic Cells ◽  
Giant Cell Astrocytoma

A 6-year-old spayed female Domestic Shorthair cat presented with a 1 to 2-month history of blindness and altered behavior. At necropsy, a 1-cm-diameter, firm white mass was found arising from the subependymal region of the right lateral ventricular wall that protruded into and partially filled the lumen. Histologically, there was a well-demarcated, expansile paraventricular neoplasm composed of moderately pleomorphic cells within a richly fibrillar matrix arranged in interlacing streams and perivascular pseudorosette-like patterns. Neoplastic cells varied in morphology from small spindloid cells to larger polygonal cells with eccentric vesicular nuclei to neuronlike cells with vesicular nuclei and prominent nucleoli. The mitotic index was low. Immunohistochemically, neoplastic cells were positive for S-100 protein, glial fibrillary acidic protein, and neuron-specific enolase and negative for neurofilament protein. Ultrastructurally, the cells contained few to abundant bundles of intermediate filaments with variable numbers of mitochondria, endoplasmic reticulum, and ribosomes. These features are characteristic of subependymal giant cell astrocytoma (SEGA) in humans. To our knowledge, this is the first reported case of SEGA in domestic animals.

scholarly journals Histochemical and Ultrastructural Characterization of Primary Cardiac Chondrosarcoma

1997 ◽  
Vol 34 (2) ◽  
pp. 150-151 ◽  
Author(s):  
T. M. Albers ◽  
J. Alroy ◽  
L. A. Garrod ◽  
D. Brown ◽  
D. Penninck
Keyword(s):  
Tumor Cells ◽  
Neuron Specific Enolase ◽  
Intercellular Junctions ◽  
Right Atrial ◽  
Golden Retriever ◽  
Neoplastic Cells ◽  
Ultrastructural Characterization ◽  
S 100 ◽  
The Right

A cardiac chondrosarcoma was found in the right atrium of a Golden Retriever dog. Macroscopically, the right atrial lumen was filled with a 6- X 12- X 8-mm white glossy mass, which was diffusely attached to the underling myocardium. The mass was composed of spindle-shaped mesenchymal neoplastic cells loosely packed in light basophilic matrix, with focal areas of tightly packed cells in linear formation similar to the pattern of a growth plate. Tumor cells were positive when stained for vimentin and neuron-specific enolase, and weakly positive for S-100 protein. Ultrastructurally, neoplastic cells had abundant, dilated rough endoplasmic reticulum, Golgi apparatus, bundles of intermediate fibers, and primitive intercellular junctions between adjacent tumor cells. Based on morphologic, ultrastructural, histochemical, and immunohistochemical characteristics, this tumor was diagnosed as a chondrosarcoma.

scholarly journals Histiocytic Sarcoma of Bone Marrow With Partial Cohesive Neoplastic Cells and Hemophagocytosis: a Case Report

2021 ◽  
Author(s):  
Yang Liang ◽  
Li Mao ◽  
Yihua Chen ◽  
Guangjie Wang
Keyword(s):  
Bone Marrow ◽  
Tumor Cells ◽  
Hemophagocytic Syndrome ◽  
Histiocytic Sarcoma ◽  
Neoplastic Cells ◽  
Case Presentation ◽  
Barr Virus ◽  
History Of ◽  
Epstein Barr ◽  
Aggressive Clinical Course

Abstract Background: Histiocytic sarcoma (HS) is a rare hematolymphoid neoplasms whose cells show morphologic and immunophenotypic features of mature tissue histiocytes. We herein report a HS case without nodules or lymphadenectasis confirmed with the help of immunohistochemistry (IHC) on bone marrow (BM) biopsy and smear.Case presentation: A 63-year-old female patient with a history of cerebral infarction presented with fever, retching and hypodynamic sign for three weeks. The peripheral blood examination showed aggressive pancytopenia accompanying with the positivity for Epstein-Barr virus (EBV) antibody. The computed tomography and abdomen ultrasound scan didn’t reveal any nodules or lymphadenectasis other than hypersplenotrophy. Significantly, the BM aspirate shows vast pleomorphic tumor cells atypically distributed in both forms of single diffuse and cohesive. The hemophagocyte phagocytized granulocytes on BM smear exhibited the lymphoma related hemophagocytic syndrome. Immunohistochemically, neoplastic cells were immunopositively for macrophage-associated antigen cluster of CD4, CD68, CD163, but negative for the T-cell, B-cell and myeloid lineage markers of CD15, CD20, PAX-5, CD5, CD30, CD3, CD56, CD38, CD138, ALK and MPO, confirming the hypothesis of HS in BM. Conclusion: The rare HS case of bone marrow with atypically partial cohesive pleomorphic tumor cells had the hemophagocytic syndrome, presented highly aggressive clinical course and challenged to the diagnoses.

scholarly journals A Rare Pulmonary Myoepithelial Tumor With Novel TRPS1-PLAG1 Fusions

2020 ◽  
Author(s):  
Qiang ZHENG ◽  
Shenglei LI ◽  
Qianming BAI ◽  
Xiaoyan ZHOU ◽  
Yue WANG ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Tumor Cells ◽  
Rna Sequencing ◽  
Clinical History ◽  
Fusion Partner ◽  
Molecular Features ◽  
First Case ◽  
Positron Emission ◽  
Eosinophilic Cytoplasm ◽  
Nodular Growth

Abstract Background: Little is known about the morphological and molecular features of intra-thoracic myoepithelial tumors. Here, we describe a rare pulmonary myoepithelial tumor with novel TRPS1-PLAG1 fusions.Case presentation: A 30-year-old male presented with multiple lung nodules that detected by routine computed tomography (CT) scan 3 years ago. Positron emission tomography-computed tomography (PET-CT) demonstrated a well-circumscribed mass with a diameter of 3cm in the left pulmonary hilum and multiple nodules in bilateral lungs. No other extra-thoracic primary lesion and distant metastasis was found. The clinical history was not other specified. Microscopically, these lesions were well-defined, showed nodular growth pattern filling the bronchioles and alveoli. The tumor cells were epithelioid to plasmacytoid with slightly eosinophilic cytoplasm, arranged in aggregates, nests and cords. Myxoid background presented in some foci. Nuclear pleomorphism was mild and mitotic activity was not prominent. No distinct gland formation was identified. Immunostains demonstrated that the tumor cells were diffusely positive for pan-cytokeratin (AE1/AE3), CK5/6, S100, SOX10, and negative for calponin. Noticeably, a TRPS1-PLAG1 gene fusion was identified in this tumor by targeted RNA sequencing involved TRPS1 exon1 and PLAG1 exon2, this rare rearrangement was validated by fluorescence in situ hybridization (FISH) for PLAG1 break-apart probe, and 30% tumor cells showed break apart signals. Conclusions: Here we present the first case of a disseminated thoracic myoepithelial tumor with novel TRPS1-PLAG1 fusions. Targeted RNA sequencing strongly facilitates precise classification and providing opportunities for unknown fusion partner discovery, which is significant for risk stratification and development of potential therapeutic targets.

scholarly journals Scrotal hemangiosarcoma in a Large White boar

2022 ◽  
Vol 52 (4) ◽  
Author(s):  
Renato Luiz Silveira ◽  
Ana Claudia de Menezes Cruz ◽  
Phillipe Bauer de Araújo Doria ◽  
Joao Marcelo Silva Silveira ◽  
Carlos Otávio de Paula Vasconcelos ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Skin Lesions ◽  
Neoplastic Cells ◽  
Large White ◽  
Existing Problems ◽  
Eosinophilic Cytoplasm ◽  
History Of ◽  
Degree Of Malignancy ◽  
Production Animals ◽  
Immunohistochemical Diagnosis

ABSTRACT: Tumors are rarely diagnosed in swine specie because of the short lifespan of production animals. Normally, these tumors do not present any clinical signs and are often detected at the time of slaughter. A 2-year-old Large White boar, used in the reproductive management of a farm and without a history of pre-existing problems, was examined for skin lesions on the scrotum. Samples were collected from skin segments containing lesions for histopathological and immunohistochemical diagnosis. Microscopically, the nodes in the scrotum pouch consisted of poorly demarcated, highly cellular, expansile, and multifocally invasive neoplasms, composed of immature endotheliocytes organized into neovascular formations. The tumor cells were pleomorphic, slightly oval to spindle-shaped, with eosinophilic cytoplasm and hyperchromatic nuclei with one to three nucleoli. All the nodules analyzed were compatible with hemangiosarcoma. After immunohistochemical evaluation, for the quantification of tissue angiogenesis the neoplastic cells immunoexpressed the CD31 monoclonal antibodies and factor VIII, through the identification of proteins expressed on the surface of endothelial cells. The Ki67 cell proliferation marker was positive in approximately 10% of the neoplastic cells, demonstrating a high degree of malignancy. Hemangiosarcoma in swine species has already been identified in several organs and tissues; however, to date, no study has demonstrated the diagnosis of this condition on the skin of the scrotum, as reported in this study. Therefore, it is expected that this report will contribute to the knowledge of the frequency of neoplasms in swine species.

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