High-resolution, ultrafast, wide-field retinal eye-tracking for enhanced quantification of fixational and saccadic motion

The digital in-line holographic microscope (DIHM) was developed for a 2D imaging technology and has recently been adapted to 3D imaging methods, providing new approaches to obtaining volumetric images with both a high resolution and wide field-of-view (FOV), which allows the physical limitations to be overcome. However, during the sectioning process of 3D image generation, the out-of-focus image of the object becomes a significant impediment to obtaining evident 3D features in the 2D sectioning plane of a thick biological sample. Based on phase retrieved high-resolution holographic imaging and a 3D deconvolution technique, we demonstrate that a high-resolution 3D volumetric image, which significantly reduces wave-front reconstruction and out-of-focus artifacts, can be achieved. The results show a 3D volumetric image that is more finely focused compared to a conventional 3D stacked image from 2D reconstructed images in relation to micron-size polystyrene beads, a whole blood smear, and a kidney tissue sample. We believe that this technology can be applicable for medical-grade images of smeared whole blood or an optically cleared tissue sample for mobile phytological microscopy and laser sectioning microscopy.

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A technique for resolution assessment in blind-SIM experiments

10.1101/2021.09.01.458550 ◽

2021 ◽

Author(s):

Imen Boujmil ◽

Giancarlo Ruocco ◽

Marco Leonetti

Keyword(s):

High Resolution ◽

Biological Sample ◽

A Priori ◽

Resolution Enhancement ◽

Super Resolution ◽

Experimental Setup ◽

Wide Field ◽

Structured Illumination ◽

Structured Illumination Microscopy ◽

Resolution Measurement

Super resolution techniques are an excellent alternative to wide field microscopy, providing high resolution also in (typically fragile) biological sample. Among the various super resolution techniques, Structured Illumination Microscopy (SIM) improve resolution by employing multiple illumination patterns to be deconvolved with a dedicated software. In the case of blind SIM techniques, unknown patterns, such as speckles, are used, thus providing super resolved images, nearly unaffected by aberrations with a simplified experimental setup. Scattering Assisted Imaging, a special blind SIM technique, exploits an illumination PSF (speckle grains size), smaller than the collection PSF (defined by the collection objectives), to surpass the typical SIM resolution enhancement. However, if SAI is used, it is very difficult to extract the resolution enhancement form a priori considerations. In this paper we propose a protocol and experimental setup for the resolution measurement, demonstrating the resolution enhancement for different collection PSF values.

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Fiber-bundle-basis sparse reconstruction for high resolution wide-field microendoscopy

Biomedical Optics Express ◽

10.1364/boe.9.001843 ◽

2018 ◽

Vol 9 (4) ◽

pp. 1843 ◽

Cited By ~ 4

Author(s):

Simon Peter Mekhail ◽

Nilupaer Abudukeyoumu ◽

Jonathan Ward ◽

Gordon Arbuthnott ◽

Síle Nic Chormaic

Keyword(s):

High Resolution ◽

Fiber Bundle ◽

Sparse Reconstruction ◽

Wide Field

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High-resolution wide-field standing-wave surface plasmon resonance fluorescence microscopy with optical vortices

Applied Physics Letters ◽

10.1063/1.3525173 ◽

2010 ◽

Vol 97 (24) ◽

pp. 241109 ◽

Cited By ~ 30

Author(s):

P. S. Tan ◽

X.-C. Yuan ◽

G. H. Yuan ◽

Q. Wang

Keyword(s):

Surface Plasmon Resonance ◽

High Resolution ◽

Fluorescence Microscopy ◽

Standing Wave ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Optical Vortices ◽

Wide Field ◽

Resonance Fluorescence ◽

Wave Surface

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Synthetic Wavelength Holography: Snapshot Non-Line-of-Sight Imaging with High-Resolution and Wide Field of View

10.21203/rs.3.rs-84906/v1 ◽

2020 ◽

Author(s):

Florian Willomitzer ◽

Prasanna Rangarajan ◽

Fengqiang Li ◽

Muralidhar Balaji ◽

Marc Christensen ◽

...

Keyword(s):

High Resolution ◽

Imaging System ◽

Scattered Light ◽

Field Of View ◽

Line Of Sight ◽

Wide Field ◽

Navigation Systems ◽

Small Probe ◽

Wide Field Of View ◽

Non Line Of Sight

Abstract The presence of a scattering medium in the imaging path between an object and an observer is known to severely limit the visual acuity of the imaging system. We present an approach to circumvent the deleterious effects of scattering, by exploiting spectral correlations in scattered wavefronts. Our Synthetic Wavelength Holography (SWH) method is able to recover a holographic representation of hidden targets with high resolution over a wide field of view. The complete object field is recorded in a snapshot-fashion, by monitoring the scattered light return in a small probe area. This unique combination of attributes opens up a plethora of new Non-Line-of-Sight imaging applications ranging from medical imaging and forensics, to early-warning navigation systems and reconnaissance. Adapting the findings of this work to other wave phenomena will help unlock a wider gamut of applications beyond those envisioned in this paper.

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Structural and Morphological Properties of Ultraluminous Infrared Galaxies at 1 < z < 3

Open Astronomy ◽

10.1515/astro-2017-0224 ◽

2015 ◽

Vol 24 (3) ◽

Author(s):

Guanwen Fang ◽

Zhongyang Ma ◽

Yang Chen ◽

Xu Kong

Keyword(s):

High Resolution ◽

Near Infrared ◽

Hubble Space Telescope ◽

Morphological Properties ◽

Wide Field ◽

High Resolution Imaging ◽

Infrared Galaxies ◽

Morphology And Structure ◽

Ultraluminous Infrared Galaxies ◽

Resolution Imaging

AbstractUsing the Hubble Space Telescope (HST)/Wide Field Camera 3 (WFC3) near-infrared high-resolution imaging from the 3D-HST survey, we analyze the morphology and structure of 502 ultraluminous infrared galaxies (ULIRGs;

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