scholarly journals Unexpectedly Stalled: Two-Photon Microscopy Reveals White Blood Cell Adhesion in Capillaries Causes Reduced Brain Blood Flow In Alzheimer’s Disease

2017 ◽  
Author(s):  
C.B. Schaffer
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Blood Flow ◽  
Cell Adhesion ◽  
Blood Cell ◽  
White Blood Cell ◽  
Brain Blood Flow ◽  
Two Photon Microscopy ◽  
Two Photon

ACE inhibitor lacks acute effect on cognition or brain blood flow in alzheimer's disease

1992 ◽  
Vol 26 (4) ◽  
pp. 467-471 ◽  
Author(s):  
Myron F. Weiner ◽  
Frederick J. Bonte ◽  
Ron Tintner ◽  
Neville Ford ◽  
Doris Svetlik ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Blood Flow ◽  
Ace Inhibitor ◽  
Acute Effect ◽  
Brain Blood Flow

scholarly journals Muramyl dipeptide mediated immunomodulation on monocyte subsets exerts therapeutic effects in a mouse model of Alzheimer’s disease

2020 ◽  
Author(s):  
Adham Fani Malekia ◽  
Giulia Cisbania ◽  
Marie-Michèle Plante ◽  
Paul Préfontaine ◽  
Nataly Laflamme ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Muramyl Dipeptide ◽  
Therapeutic Effects ◽  
Monocyte Subsets ◽  
Expression Levels ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Model Of Alzheimer’S Disease

Abstract Background Muramyl dipeptide (MDP) is a component derived from minimal peptidoglycan motif from bacteria and it is a ligand for the NOD2 receptor. Peripheral administration of MDP converts Ly6Chigh into Ly6Clow monocytes. Previously we have shown that Ly6Clow monocytes play crucial roles in the pathology of a mouse model of Alzheimer’s disease (AD). However, medications with mild immunomodulatory effects that solely target specific monocyte subsets, without triggering microglial activation are rare. Methods 3-months old APPswe/PS1 transgenic male mice and age-matched C57BL/6J mice were used for high frequency (2-times/week) over 6-months and low frequency (once a week) over a 3-months period of intraperitoneally MDP (10 mg/kg) administrations. Flow cytometry analysis of monocyte subsets in blood, behavioral and post mortem analyses were performed. Two-photon microscopy using APP/PS1/CX3CR1gfp/+ mice were conducted to study vascular Aβ clearance by Ly6Clow monocytes upon MDP administration.Results The treatment improved cognitive declines, increased expression levels of postsynaptic density protein 95 (PSD95) andlow density lipoprotein receptor-related protein 1 (LRP1), which are involved in synaptic plasticity and amyloid beta (Aβ) elimination, respectively. In addition, we found monocyte chemoattractant protein-1(MCP-1) levels significantly increased, whereas intercellular adhesion molecule-1(ICAM-1) significantly decreased and microglial marker (Iba1) did not change in treatment group compared to the control. In parallel, we discovered elevated cyclooxygenase-2 (COX2) expression levels in the treated group, which might be a positive factor for synaptic activity. Following MDP treatment, intravital two-photon microscopy demonstrated that Ly6Clow monocytes are recruited into the brain vasculature in APP but not wild type mice, and they are able to pick up Aβ peptides. Conclusions Our results demonstrate that MDP is beneficial in both the early phase and to some extent later phases of the pathology in the mouse model of AD. These data open the way for potential MDP-based medications for AD.

P4-027: Kinetics of dendritic structural plasticity in the living brain of an Alzheimer's disease mouse model acquired with two photon microscopy

2009 ◽  
Vol 5 (4S_Part_14) ◽  
pp. P439-P439
Author(s):  
Steffen Burgold ◽  
Martin Fuhrmann ◽  
Boris Schmidt ◽  
Hans Kretzschmar ◽  
Jochen Herms
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Structural Plasticity ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Kinetics Of

scholarly journals Whole brain vascular imaging in a mouse model of Alzheimer’s disease with two-photon microscopy

2018 ◽  
Vol 23 (07) ◽  
pp. 1 ◽  
Author(s):  
Patrick Delafontaine-Martel ◽  
Joel Lefebvre ◽  
Pier-Luc Tardif ◽  
Bernard I. Lévy ◽  
Philippe Pouliot ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Vascular Imaging ◽  
Whole Brain ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Model Of Alzheimer’S Disease

scholarly journals Unexpectedly stalled: the dynamics of brain blood flow in Alzheimer’s disease

2019 ◽  
Vol 05 (03) ◽  
Author(s):  
Oliver Bracko ◽  
Nozomi Nishimura ◽  
Chris B. Schaffer
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Blood Flow ◽  
Brain Blood Flow
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