Megapixel Fluorescence Lifetime Imaging by Super-Resolution of a SPAD array

2020 ◽  
Author(s):  
Yongzhuang Zhou ◽  
Graham Hungerford ◽  
Guillem Carles ◽  
Vytautas Zickus ◽  
David McLoskey ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Fluorescence axial nanotomography with plasmonics

2015 ◽  
Vol 178 ◽  
pp. 371-381 ◽  
Author(s):  
Nicholas I. Cade ◽  
Gilbert O. Fruhwirth ◽  
Alexey V. Krasavin ◽  
Tony Ng ◽  
David Richards
Keyword(s):  
Fluorescence Lifetime ◽  
Cell Imaging ◽  
Super Resolution ◽  
Cellular Membrane ◽  
Carcinoma Cells ◽  
Axial Position ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Position Sensitivity ◽  
Confocal Fluorescence

We present a novel imaging technique with super-resolution axial sensitivity, exploiting the changes in fluorescence lifetime above a plasmonic substrate. Using conventional confocal fluorescence lifetime imaging, we show that it is possible to deliver down to 6 nm axial position sensitivity of fluorophores in whole biological cell imaging. We employ this technique to map the topography of the cellular membrane, and demonstrate its application in an investigation of receptor-mediated endocytosis in carcinoma cells.

Super-resolution Fluorescence Lifetime Imaging

2012 ◽  
Author(s):  
Zhaotai Gu ◽  
Cuifang Kuang ◽  
Shuai Li ◽  
Yi Xue ◽  
Zhenrong Zheng ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Multiphoton fluorescence lifetime imaging microscopy (FLIM) and super-resolution fluorescence imaging with a supramolecular biopolymer for the controlled tagging of polysaccharides

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9498-9507 ◽  
Author(s):  
Haobo Ge ◽  
Fernando Cortezon-Tamarit ◽  
Hui-Chen Wang ◽  
Adam C. Sedgwick ◽  
Rory L. Arrowsmith ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Boronate Ester ◽  
Lifetime Imaging ◽  
In Cells

A new coumarin-appended boronate ester for fluorogenic imaging which binds polysaccharides in solution and in cells.

scholarly journals High-speed, long-term, 4D in vivo lifetime imaging in intact and injured zebrafish and mouse brains by instant FLIM

2020 ◽  
Author(s):  
Yide Zhang ◽  
Ian H. Guldner ◽  
Evan L. Nichols ◽  
David Benirschke ◽  
Cody J. Smith ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Fluorescence Lifetime ◽  
High Speed ◽  
Super Resolution ◽  
Cost Effective ◽  
Fluorescence Lifetime Imaging ◽  
Imaging Data ◽  
Lifetime Imaging ◽  
Effective Components

AbstractTraditional fluorescence microscopy is blind to molecular microenvironment information that is present in fluorescence lifetime, which can be measured by fluorescence lifetime imaging microscopy (FLIM). However, existing FLIM techniques are typically slow to acquire and process lifetime images, difficult to implement, and expensive. Here, we present instant FLIM, an analog signal processing method that allows real-time streaming of fluorescence intensity, lifetime, and phasor imaging data through simultaneous image acquisition and instantaneous data processing. Instant FLIM can be easily implemented by upgrading an existing two-photon microscope using cost-effective components and our open-source software. We further improve the functionality, penetration depth, and resolution of instant FLIM using phasor segmentation, adaptive optics, and super-resolution techniques. We demonstrate through-skull intravital 3D FLIM of mouse brains to depths of 300 μm and present the first in vivo 4D FLIM of microglial dynamics in intact and injured zebrafish and mouse brains up to 12 hours.

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