The aerial parts of three endemic Centaurea L. species, namely C. tomorosii
Micevski, C. soskae Hayek and C. galicicae Micevski, afforded sesquiterpene
lactone cnicin (1) and seven flavonoids: apigenin (2), isokaempferide (3),
hispidulin (4), eupatorin (5), cirsimaritin (6), santaflavone (7) and
salvigenin (8). The structures of the isolated compounds were determined by
UV, 1H NMR and 13C NMR spectroscopy and HR-ESI-MS spectrometry. 1H NMR
spectroscopy was used as a method for quantitative analysis of sesquiterpene
lactone cnicin.