Differential tissue growth and cell adhesion alone drive early tooth morphogenesis: An ex vivo and in silico study

Telomeres protect chromosomal ends and they are maintained by the specialised enzyme, telomerase. Endometriosis is a common gynaecological disease and high telomerase activity and higher hTERT levels associated with longer endometrial telomere lengths are characteristics of eutopic secretory endometrial aberrations of women with endometriosis. Our ex-vivo study examined the levels of hTERC and DKC1 RNA and dyskerin protein levels in the endometrium from healthy women and those with endometriosis (n = 117). The in silico study examined endometriosis-specific telomere- and telomerase-associated gene (TTAG) transcriptional aberrations of secretory phase eutopic endometrium utilising publicly available microarray datasets. Eutopic secretory endometrial hTERC levels were significantly increased in women with endometriosis compared to healthy endometrium, yet dyskerin mRNA and protein levels were unperturbed. Our in silico study identified 10 TTAGs (CDKN2A, PML, ZNHIT2, UBE3A, MCCC2, HSPC159, FGFR2, PIK3C2A, RALGAPA1, and HNRNPA2B1) to be altered in mid-secretory endometrium of women with endometriosis. High levels of hTERC and the identified other TTAGs might be part of the established alteration in the eutopic endometrial telomerase biology in women with endometriosis in the secretory phase of the endometrium and our data informs future research to unravel the fundamental involvement of telomerase in the pathogenesis of endometriosis.

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A selective defect in Fas-mediated apoptosis in HAM/TSP: An ex vivo, in vitro and in silico study

Retrovirology ◽

10.1186/1742-4690-11-s1-p78 ◽

2014 ◽

Vol 11 (Suppl 1) ◽

pp. P78 ◽

Cited By ~ 1

Author(s):

Soraya Menezes ◽

Daniele Decanine ◽

Ricardo Khouri ◽

Saul Schnitman ◽

Ramon A Kruschewsky ◽

...

Keyword(s):

In Silico ◽

Ex Vivo ◽

In Silico Study

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Crassocephalum rubens , a leafy vegetable, suppresses oxidative pancreatic and hepatic injury and inhibits key enzymes linked to type 2 diabetes: An ex vivo and in silico study

Journal of Food Biochemistry ◽

10.1111/jfbc.12930 ◽

2019 ◽

Vol 43 (8) ◽

Author(s):

Olajumoke Arinola Oyebode ◽

Ochuko Lucky Erukainure ◽

Collins Ibeji ◽

Neil Anthony Koorbanally ◽

Md. Shahidul Islam

Keyword(s):

Type 2 Diabetes ◽

In Silico ◽

Hepatic Injury ◽

Ex Vivo ◽

Leafy Vegetable ◽

Key Enzymes ◽

In Silico Study

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The Effect of Glargine As Basal Insulin Support for Recovering Critically Ill and High Dependency Unit Patients: An in Silico Study

7th IFAC Symposium on Modelling and Control in Biomedical Systems ◽

10.3182/20090812-3-dk-2006.00009 ◽

2009 ◽

Author(s):

Lin, Jessica

Keyword(s):

Critically Ill ◽

In Silico ◽

Basal Insulin ◽

High Dependency Unit ◽

High Dependency ◽

In Silico Study

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Assessing Drug Transport Across the Human Placental Barrier: From In Vivo and In Vitro Measurements to the Ex Vivo Perfusion Method and In silico Techniques

Current Pharmaceutical Biotechnology ◽

10.2174/138920111795470930 ◽

2011 ◽

Vol 12 (5) ◽

pp. 804-813 ◽

Cited By ~ 11

Author(s):

Constantinos Giaginis ◽

Anna Tsantili-Kakoulidou ◽

Stamatios Theocharis

Keyword(s):

In Silico ◽

Drug Transport ◽

Ex Vivo ◽

Placental Barrier ◽

Ex Vivo Perfusion ◽

Perfusion Method ◽

In Vitro Measurements

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In Silico Study of Structural and Geometrical Requirements of Natural Sesquiterpene Lactones with Trypanocidal Activity

Mini-Reviews in Medicinal Chemistry ◽

10.2174/13895575113139990066 ◽

2013 ◽

Vol 13 (10) ◽

pp. 1407-1414 ◽

Cited By ~ 10

Author(s):

L. Fabian ◽

V. Sulsen ◽

F. Frank ◽

S. Cazorla ◽

E. Malchiodi ◽

...

Keyword(s):

In Silico ◽

Sesquiterpene Lactones ◽

Trypanocidal Activity ◽

In Silico Study

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In Vitro, In Silico and Ex Vivo Studies of Dihydroartemisinin Derivatives as Antitubercular Agents

Current Topics in Medicinal Chemistry ◽

10.2174/1568026619666190305131425 ◽

2019 ◽

Vol 19 (8) ◽

pp. 633-644 ◽

Cited By ~ 1

Author(s):

Komal Kalani ◽

Sarfaraz Alam ◽

Vinita Chaturvedi ◽

Shyam Singh ◽

Feroz Khan ◽

...

Keyword(s):

Bone Marrow ◽

In Silico ◽

Ex Vivo ◽

Virulent Strain ◽

Infection Model ◽

Mouse Bone Marrow ◽

Significant Activity ◽

Macrophage Infection ◽

In Silico Studies

Introduction: As a part of our drug discovery program for anti-tubercular agents, dihydroartemisinin (DHA-1) was screened against Mtb H37Rv, which showed moderate anti-tubercular activity (>25.0 µg/mL). These results prompted us to carry out the chemical transformation of DHA-1 into various derivatives and study their antitubercular potential. Materials and Methods: DHA-1 was semi-synthetically converted into four new acyl derivatives (DHA-1A – DHA-1D) and in-vitro evaluated for their anti-tubercular potential against Mycobacterium tuberculosis H37Rv virulent strain. The derivatives, DHA-1C (12-O-(4-nitro) benzoyl; MIC 12.5 µg/mL) and DHA-1D (12-O-chloro acetyl; MIC 3.12µg/mL) showed significant activity against the pathogen. Results: In silico studies of the most active derivative (DHA-1D) showed interaction with ARG448 inhibiting the mycobacterium enzymes. Additionally, it showed no cytotoxicity towards the Vero C1008 cells and Mouse bone marrow derived macrophages. Conclusion: DHA-1D killed 62% intracellular M. tuberculosis in Mouse bone marrow macrophage infection model. To the best of our knowledge, this is the first-ever report on the antitubercular potential of dihydroartemisinin and its derivatives. Since dihydroartemisinin is widely used as an antimalarial drug; these results may be of great help in anti-tubercular drug development from a very common, inexpensive, and non-toxic natural product.

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In Silico Study of 1, 4 Alpha Glucan Branching Enzyme and Substrate Docking Studies

Current Proteomics ◽

10.2174/1570164616666190401204009 ◽

2020 ◽

Vol 17 (1) ◽

pp. 40-50

Author(s):

Farzane Kargar ◽

Amir Savardashtaki ◽

Mojtaba Mortazavi ◽

Masoud Torkzadeh Mahani ◽

Ali Mohammad Amani ◽

...

Keyword(s):

Phylogenetic Tree ◽

In Silico ◽

Alpha Helix ◽

In Silico Analysis ◽

Glycogen Storage ◽

Docking Studies ◽

Random Coil ◽

Special Topic ◽

Industrial Biotechnology ◽

In Silico Study

Background: The 1,4-alpha-glucan branching protein (GlgB) plays an important role in the glycogen biosynthesis and the deficiency in this enzyme has resulted in Glycogen storage disease and accumulation of an amylopectin-like polysaccharide. Consequently, this enzyme was considered a special topic in clinical and biotechnological research. One of the newly introduced GlgB belongs to the Neisseria sp. HMSC071A01 (Ref.Seq. WP_049335546). For in silico analysis, the 3D molecular modeling of this enzyme was conducted in the I-TASSER web server. Methods: For a better evaluation, the important characteristics of this enzyme such as functional properties, metabolic pathway and activity were investigated in the TargetP software. Additionally, the phylogenetic tree and secondary structure of this enzyme were studied by Mafft and Prabi software, respectively. Finally, the binding site properties (the maltoheptaose as substrate) were studied using the AutoDock Vina. Results: By drawing the phylogenetic tree, the closest species were the taxonomic group of Betaproteobacteria. The results showed that the structure of this enzyme had 34.45% of the alpha helix and 45.45% of the random coil. Our analysis predicted that this enzyme has a potential signal peptide in the protein sequence. Conclusion: By these analyses, a new understanding was developed related to the sequence and structure of this enzyme. Our findings can further be used in some fields of clinical and industrial biotechnology.

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