scholarly journals Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration

PLoS Genetics ◽  
2021 ◽  
Vol 17 (12) ◽  
pp. e1009948
Author(s):  
Pooja Flora ◽  
Meng-Yen Li ◽  
Phillip M. Galbo ◽  
Maider Astorkia ◽  
Deyou Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Polycomb Repressive Complex ◽  
Polycomb Repressive Complex 2 ◽  
Activated State ◽  
Hair Regeneration ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Hair follicle stem cells (HFSCs) are multipotent cells that cycle through quiescence and activation to continuously fuel the production of hair follicles. Prior genome mapping studies had shown that tri-methylation of histone H3 at lysine 27 (H3K27me3), the chromatin mark mediated by Polycomb Repressive Complex 2 (PRC2), is dynamic between quiescent and activated HFSCs, suggesting that transcriptional changes associated with H3K27me3 might be critical for proper HFSC function. However, functional in vivo studies elucidating the role of PRC2 in adult HFSCs are lacking. In this study, by using in vivo loss-of-function studies we show that, surprisingly, PRC2 plays a non-instructive role in adult HFSCs and loss of PRC2 in HFSCs does not lead to loss of HFSC quiescence or changes in cell identity. Interestingly, RNA-seq and immunofluorescence analyses of PRC2-null quiescent HFSCs revealed upregulation of genes associated with activated state of HFSCs. Altogether, our findings show that transcriptional program under PRC2 regulation is dispensable for maintaining HFSC quiescence and hair regeneration.

scholarly journals Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632

2021 ◽  
Vol 9 ◽  
Author(s):  
Lihong Wen ◽  
Yong Miao ◽  
Zhexiang Fan ◽  
Jiarui Zhang ◽  
Yixuan Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Culture System ◽  
Hair Follicles ◽  
Human Hair Follicle ◽  
Rock Inhibitor ◽  
Hair Follicle Stem Cells ◽  
Human Fibronectin ◽  
Follicle Stem Cells

BackgroundHair follicle tissue engineering is a promising strategy for treating hair loss. Human hair follicle stem cells (hHFSCs), which play a key role in the hair cycle, have potential applications in regenerative medicine. However, previous studies did not achieve efficient hHFSC expansion in vitro using feeder cells. Therefore, there is a need to develop an efficient primary culture system for the expansion and maintenance of hHFSCs.MethodsThe hHFSCs were obtained by two-step proteolytic digestion combined with microscopy. The cell culture dishes were coated with human fibronectin and inoculated with hHFSCs. The hHFSCs were harvested using a differential enrichment procedure. The effect of Rho-associated protein kinase (ROCK) inhibitor Y-27632, supplemented in keratinocyte serum-free medium (K-SFM), on adhesion, proliferation, and stemness of hHFSCs and the underlying molecular mechanisms were evaluated.ResultsThe hHFSCs cultured in K-SFM, supplemented with Y-27632, exhibited enhanced adhesion and proliferation. Additionally, Y-27632 treatment maintained the stemness of hHFSCs and promoted the ability of hHFSCs to regenerate hair follicles in vivo. However, Y-27632-induced proliferation and stemness in hHFSCs were conditional and reversible. Furthermore, Y-27632 maintained propagation and stemness of hHFSCs through the ERK/MAPK pathway.ConclusionAn efficient short-term culture system for primary hHFSCs was successfully established using human fibronectin and the ROCK inhibitor Y-27632, which promoted the proliferation, maintained the stemness of hHFSCs and promoted the ability to regenerate hair follicles in vivo. The xenofree culturing method used in this study provided a large number of high-quality seed cells, which have applications in hair follicle tissue engineering and stem cell therapy.

scholarly journals A Wnt5a-Cdc42 axis controls aging and rejuvenation of hair-follicle stem cells

2020 ◽  
Author(s):  
Rajiv L Tiwari ◽  
Pratibha Mishra ◽  
Nicola Martin ◽  
Nikhil Oommen George ◽  
Vadim Sakk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wnt Signaling ◽  
Hair Follicle ◽  
Canonical Wnt Signaling ◽  
Hair Regeneration ◽  
Hair Follicle Stem Cells ◽  
Elevated Activity ◽  
Canonical Wnt ◽  
Follicle Stem Cells

SummaryNormal hair growth occurs in cycles, comprising growth (anagen), cessation (catagen) and rest (telogen). Upon aging, the initiation of anagen is significantly delayed, which results in impaired hair regeneration. Hair regeneration is driven by hair follicle stem cells (HFSCs). We show here that aged HFSCs present with a decrease in canonical Wnt signaling and a shift towards non-canonical Wnt5a driven signaling which antagonizes canonical Wnt signaling. Elevated expression of Wnt5a in HFSCs upon aging results in elevated activity of the small RhoGTPase Cdc42 as well as a change in the spatial distribution of Cdc42 within HFSCs. Treatment of aged HFSC with a specific pharmacological inhibitor of Cdc42 activity termed CASIN to suppress the aging-associated elevated activity of Cdc42 restored canonical Wnt signaling in aged HFSCs. Treatment of aged mice in vivo with CASIN induced anagen onset and increased the percentage of anagen skin areas. Aging-associated functional deficits of HFSCs are at least in part intrinsic to HFSCs and can be restored by rational pharmacological approaches.

scholarly journals A Wnt5a-Cdc42 axis controls aging and rejuvenation of hair-follicle stem cells

2020 ◽  
Author(s):  
Rajiv Tiwari ◽  
Pratibha Mishra ◽  
Nicola Martin ◽  
Nikhil George ◽  
Vadim Sakk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wnt Signaling ◽  
Hair Follicle ◽  
Canonical Wnt Signaling ◽  
Hair Regeneration ◽  
Hair Follicle Stem Cells ◽  
Elevated Activity ◽  
Canonical Wnt ◽  
Follicle Stem Cells

Abstract Normal hair growth occurs in cycles, comprising growth (anagen), cessation (catagen) and rest (telogen). Upon aging, the initiation of anagen is significantly delayed, which results in impaired hair regeneration. Hair regeneration is driven by hair follicle stem cells (HFSCs). We show here that aged HFSCs present with a decrease in canonical Wnt signaling and a shift towards non-canonical Wnt5a driven signaling which antagonizes canonical Wnt signaling. Elevated expression of Wnt5a in HFSCs upon aging results in elevated activity of the small RhoGTPase Cdc42 as well as a change in the spatial distribution of Cdc42 within HFSCs. Treatment of aged HFSC with a specific pharmacological inhibitor of Cdc42 activity termed CASIN to suppress the aging-associated elevated activity of Cdc42 restored canonical Wnt signaling in aged HFSCs. Treatment of aged mice in vivo with CASIN induced anagen onset and increased the percentage of anagen skin areas. Aging-associated functional deficits of HFSCs are at least in part intrinsic to HFSCs and can be restored by rational pharmacological approaches.

Hair Follicle Stem Cells Derived from Single Rat Vibrissa via Organ Culture Reconstitute Hair Follicles in Vivo

2012 ◽  
Vol 21 (6) ◽  
pp. 1075-1085 ◽  
Author(s):  
Shoubing Zhang ◽  
Huimin Hu ◽  
Huishan Zhang ◽  
Shuang Liu ◽  
Shu Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Organ Culture ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Hair Follicle Stem Cells and Hair Regeneration

2020 ◽  
pp. 1-32
Author(s):  
Sung-Jan Lin ◽  
Wen-Yen Huang ◽  
Chih-Chiang Chen ◽  
Mingxing Lei ◽  
Jin-Bon Hong
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Hair Regeneration ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Expansion of Hair Follicle Stem Cells Sticking to Isolated Sebaceous Glands to Generate in Vivo Epidermal Structures

2016 ◽  
Vol 25 (11) ◽  
pp. 2071-2082 ◽  
Author(s):  
Huishan Zhang ◽  
Huashan Zhao ◽  
Jingqiao Qiao ◽  
Shoubing Zhang ◽  
Shuang Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Sebaceous Glands ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

scholarly journals Mechanotransductive Differentiation of Hair Follicle Stem Cells Derived from Aged Eyelid Skin into Corneal Endothelial-Like Cells

2021 ◽  
Author(s):  
Christian Olszewski ◽  
Jessika Maassen ◽  
Rebecca Guenther ◽  
Claudia Skazik-Voogt ◽  
Angela Gutermuth
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Adherent Cell ◽  
Promising Alternative ◽  
Eyelid Skin ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

AbstractCorneal endothelial insufficiency is one of the leading causes of blindness. The main contemporary treatment for corneal blindness is endothelial keratoplasty, which, however, is unsatisfactory as a medical therapy due to the lack of donor corneas and graft rejection. Therefore, autologous stem cell-based corneal endothelial tissue substitutes may be a promising alternative to conventional grafts in the future. To address the age of most patients suffering from corneal endothelial deficiencies, we investigated the presence and potential of hair-derived stem cells from older tissue donors. Our studies revealed the presence of pluripotency- and neural crest-associated markers in tissue sections from blepharoplasty patients aged 50 to 80 years. In vitro outgrowths from eyelid hair follicles on collagen-coated tissue culture plates revealed a weak decrease in stem-cell potency. In contrast, cells within the spheres that spontaneously formed from the adherent cell layer retained full stem-cell potency and could be differentiated into cells of the ecto- meso and endodermal lineages. Although these highly potent hair follicle derived stem cells (HFSC) were only very slightly expandable, they were able to recognize the biomimicry of the Descemet’s-like topography and differentiate into corneal endothelial-like cells. In conclusion, HFSCs derived from epidermal skin of eyelid biopsies are a promising cell source to provide autologous corneal endothelial replacement for any age group of patients. Graphical Abstract

scholarly journals Adult hair follicles keep oncogenic growth in check

2019 ◽  
Vol 218 (10) ◽  
pp. 3163-3165
Author(s):  
Denise Gay ◽  
Mayumi Ito
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Somatic Mutations ◽  
Hair Follicles ◽  
Management Technique ◽  
Normal Cells ◽  
Cell Biol ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

Recent research shows that potentially cancerous, somatic mutations can reside in normal cells. Pineda et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201907178) report on a unique management technique by hair follicle stem cells to evade tumorigenesis.

scholarly journals Targeted inactivation of integrin-linked kinase in hair follicle stem cells reveals an important modulatory role in skin repair after injury

2011 ◽  
Vol 22 (14) ◽  
pp. 2532-2540 ◽  
Author(s):  
Kerry-Ann Nakrieko ◽  
Alena Rudkouskaya ◽  
Timothy S. Irvine ◽  
Sudhir J. A. D'souza ◽  
Lina Dagnino
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Stem Cell Population ◽  
Epidermal Surface ◽  
Hair Follicle Stem Cells ◽  
Integrin Linked Kinase ◽  
Hair Follicle Growth ◽  
Follicle Stem Cells

Integrin-linked kinase (ILK) is key for normal epidermal morphogenesis, but little is known about its role in hair follicle stem cells and epidermal regeneration. Hair follicle stem cells are important contributors to newly formed epidermis following injury. We inactivated the Ilk gene in the keratin 15–expressing stem cell population of the mouse hair follicle bulge. Loss of ILK expression in these cells resulted in impaired cutaneous wound healing, with substantially decreased wound closure rates. ILK-deficient stem cells produced very few descendants that moved toward the epidermal surface and into the advancing epithelium that covers the wound. Furthermore, those few mutant cells that homed in the regenerated epidermis exhibited a reduced residence time. Paradoxically, ILK-deficient bulge stem cells responded to anagen growth signals and contributed to newly regenerated hair follicles during this phase of hair follicle growth. Thus ILK plays an important modulatory role in the normal contribution of hair follicle stem cell progeny to the regenerating epidermis following injury.

Hair Follicle Stem Cells and Hair Regeneration

2020 ◽  
pp. 265-296
Author(s):  
Sung-Jan Lin ◽  
Wen-Yen Huang ◽  
Chih-Chiang Chen ◽  
Mingxing Lei ◽  
Jin-Bon Hong
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Hair Regeneration ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells
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