scholarly journals Induction of systemic, mucosal and memory antibody responses targeting Vibrio cholerae O1 O-specific polysaccharide (OSP) in adults following oral vaccination with an oral killed whole cell cholera vaccine in Bangladesh

2019 ◽  
Vol 13 (8) ◽  
pp. e0007634 ◽  
Author(s):  
Aklima Akter ◽  
Pinki Dash ◽  
Amena Aktar ◽  
Sultana Rownok Jahan ◽  
Sadia Afrin ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Antibody Responses ◽  
Cholera Vaccine ◽  
Oral Vaccination ◽  
Whole Cell ◽  
Specific Polysaccharide ◽  
Vibrio Cholerae O1

scholarly journals Vibrio cholerae O1 Ogawa detoxified lipopolysaccharide structures as inducers of cytokines and oxidative species in macrophages

2010 ◽  
Vol 59 (2) ◽  
pp. 158-164 ◽  
Author(s):  
Ema Paulovičová ◽  
Elena Kováčová ◽  
Slavomír Bystrický
Keyword(s):  
Vibrio Cholerae ◽  
Ex Vivo ◽  
Peritoneal Macrophages ◽  
Cholera Vaccine ◽  
Specific Polysaccharide ◽  
Vibrio Cholerae O1 ◽  
Mouse Peritoneal Macrophages ◽  
Oxidative Species ◽  
Necrosis Factor ◽  
Pro Inflammatory Cytokines

Multidrug resistance in several strains of Vibrio cholerae has encouraged anti-cholera vaccine developmental attempts using various subcellular moieties. In order to examine the immunological efficacy of detoxified LPS (dLPS)-derived saccharide immunogens, ex vivo activation of mouse peritoneal macrophages (MΦs) was investigated. The immunomodulatory effect was evaluated via induction of the pro-inflammatory cytokines tumour necrosis factor-α, interleukin (IL)-1α and IL-6 and acceleration of nitric oxide (NO) and reactive oxygen species (ROS). Immunologically active structures triggered mouse peritoneal MΦs to secrete cytokines and release NO/ROS, even at concentrations as low as 12.5 μg ml−1. It was found that the O-specific polysaccharide moiety was more immunologically efficient than the glycolipid one, probably due to the position of 3-deoxy-d-manno-octulosonic acid. The results revealed effective structure–immunomodulating relationships of dLPS-derived moieties that are desirable in subcellular anti-cholera vaccine design.

scholarly journals Transcutaneous Immunization with a Vibrio cholerae O1 Ogawa Synthetic Hexasaccharide Conjugate following Oral Whole-Cell Cholera Vaccination Boosts Vibriocidal Responses and Induces Protective Immunity in Mice

2012 ◽  
Vol 19 (4) ◽  
pp. 594-602 ◽  
Author(s):  
A. A. Tarique ◽  
A. Kalsy ◽  
M. Arifuzzaman ◽  
S. M. Rollins ◽  
R. C. Charles ◽  
...  
Keyword(s):  
Immune Responses ◽  
Vibrio Cholerae ◽  
Protective Immunity ◽  
Protective Efficacy ◽  
Immune Serum ◽  
Whole Cell ◽  
Content Type ◽  
Specific Polysaccharide ◽  
Subcutaneous Immunization ◽  
Cholera Vaccination

ABSTRACTA shortcoming of currently available oral cholera vaccines is their induction of relatively short-term protection against cholera compared to that afforded by wild-type disease. We were interested in whether transcutaneous or subcutaneous boosting using a neoglycoconjugate vaccine made from a synthetic terminal hexasaccharide of the O-specific polysaccharide ofVibrio choleraeO1 (Ogawa) coupled to bovine serum albumin as a carrier (CHO-BSA) could boost lipopolysaccharide (LPS)-specific and vibriocidal antibody responses and result in protective immunity following oral priming immunization with whole-cell cholera vaccine. We found that boosting with CHO-BSA with immunoadjuvantative cholera toxin (CT) orEscherichia coliheat-labile toxin (LT) following oral priming with attenuatedV. choleraeO1 vaccine strain O395-NT resulted in significant increases in serum anti-V. choleraeLPS IgG, IgM, and IgA (P< 0.01) responses as well as in anti-Ogawa (P< 0.01) and anti-Inaba (P< 0.05) vibriocidal titers in mice. The LPS-specific IgA responses in stool were induced by transcutaneous (P< 0.01) but not subcutaneous immunization. Immune responses following use of CT or LT as an adjuvant were comparable. In a neonatal mouse challenge assay, immune serum from boosted mice was associated with 79% protective efficacy against death. Our results suggest that transcutaneous and subcutaneous boosting with a neoglycoconjugate following oral cholera vaccination may be an effective strategy to prolong protective immune responses againstV. cholerae.

scholarly journals Immune responses to O-specific polysaccharide (OSP) in North American adults infected with Vibrio cholerae O1 Inaba

2019 ◽  
Vol 13 (11) ◽  
pp. e0007874 ◽  
Author(s):  
Motaher Hossain ◽  
Kamrul Islam ◽  
Meagan Kelly ◽  
Leslie M. Mayo Smith ◽  
Richelle C. Charles ◽  
...  
Keyword(s):  
Immune Responses ◽  
Vibrio Cholerae ◽  
North American ◽  
Specific Polysaccharide ◽  
Vibrio Cholerae O1

scholarly journals Immunological efficacy of glycoconjugates derived from Vibrio cholerae O1 serotype Ogawa detoxified LPS in mice

2010 ◽  
Vol 59 (12) ◽  
pp. 1440-1448 ◽  
Author(s):  
Ema Paulovičová ◽  
Jana Korcová ◽  
Pavol Farkaš ◽  
Slavomír Bystrický
Keyword(s):  
B Cell ◽  
Vibrio Cholerae ◽  
Primary Vaccination ◽  
Receptor Expression ◽  
T Helper 1 ◽  
Phagocytic Capacity ◽  
Humoral And Cellular Immunity ◽  
Significant Acceleration ◽  
Specific Polysaccharide ◽  
Vibrio Cholerae O1

This study focused on changes in selected parameters of humoral and cellular immunity following vaccination of mice with unique Vibrio cholerae LPS–protein-complexed conjugates. The V. cholerae detoxified LPS (dLPS)-derived antigenic structures O-specific polysaccharide (O-SP) and de-O-acylated LPS (DeOAc-LPS) were used to prepare glycoconjugates by linking both dLPSs to glucan, the immunomodulating matrix, and then to BSA carrier. Animals were given a primary vaccination and boosted at 2-week intervals with a dose of 4.5 μg saccharide antigen. The last boost was given either subcutaneously or intraperitoneally (i.p.) to compare the boosting effect and to optimize the effective immunization route. Both conjugates (O-SP–BSA and DeOAc-LPS–BSA) induced significant levels of antigen-specific Ig isotypes, especially IgG and IgM. The i.p. booster route was more effective. A T helper 1 response was achieved only by immunization with O-SP–BSA conjugate administered i.p. Significant acceleration of phagocytic capacity and respiratory burst of neutrophils was demonstrated by both immunogenic formulations. Activation of T- and B-cell adaptive immunities was exhibited as specific changes in CD3 : CD19 and CD4 : CD8 ratios, B-cell low-affinity Fcγ II and III receptor expression and induction of CD45R antigen.

scholarly journals Evaluation of antibody responses to outer membrane vesicles (OMVs) and killed whole cell of Vibrio cholerae O1 El Tor in immunized mice

2019 ◽  
Author(s):  
Manijeh Sedaghat ◽  
Seyed Davar Siadat ◽  
Esmat Mirabzadeh ◽  
Malihe Keramati ◽  
Farzam Vaziri ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Vibrio Cholerae ◽  
Membrane Vesicles ◽  
Intestinal Secretion ◽  
Oral Immunization ◽  
Outer Membrane Vesicles ◽  
Clinical Strain ◽  
Whole Cell ◽  
Vibrio Cholerae O1 ◽  
El Tor

Background and Objectives: Cholera disease remains an important global health problem affecting 3-5 million subjects worldwide. Outer membrane vesicles (OMVs) have been found in a variety of Gram-negative bacteria and act as protective transport vesicles. The aim of this study was to evaluate Immune responses against Vibrio cholerae O1 El Tor clinical strain OMV and compare it with killed whole cell (KWC), complex of (KWC-OMV) as well as the internationally licensed oral cholera vaccine, Dukoral, in serum and intestinal secretions of mice. Materials and Methods: OMVs were prepared by using modified detergent-centrifugation procedure from V. cholerae O1 El Tor clinical strain from 2005 outbreak. The ultrastructure and content of OMVs were investigated via the Scanning Elec- tron Microscopy (SEM) and SDS-PAGE analysis. Three doses of oral immunization were adjusted and total IgG and IgA in serum and intestinal secretion were measured by enzyme-linked immunosorbent assay (ELISA). Results: Extracted OMVs from the V. cholerae were spherical vesicles with a size ranging from 10 to 300 nm. OMV-im- munized mice showed an increased level of total IgG and IgA both in serum and intestinal secretion when compared to the negative controls. Also, there existed a higher level of secretory IgA than the total IgG, suggesting the most of protection against V. cholerae colonization provided by sIgA. Conclusion: Our findings revealed that oral immunization with V. cholerae OMVs might induce a long-term immunity, es- pecially when administered in combination with KWC. This study tested the adjuvant activity of OMVs and may be useful in future nano vaccine research.

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