Synthesis and Molecular Structure of the  5-Methoxycarbonylpentyl α-Glycoside of the Upstream, Terminal Moiety of the O-Specific Polysaccharide of  Vibrio cholerae O1, Serotype Inaba

Multidrug resistance in several strains of Vibrio cholerae has encouraged anti-cholera vaccine developmental attempts using various subcellular moieties. In order to examine the immunological efficacy of detoxified LPS (dLPS)-derived saccharide immunogens, ex vivo activation of mouse peritoneal macrophages (MΦs) was investigated. The immunomodulatory effect was evaluated via induction of the pro-inflammatory cytokines tumour necrosis factor-α, interleukin (IL)-1α and IL-6 and acceleration of nitric oxide (NO) and reactive oxygen species (ROS). Immunologically active structures triggered mouse peritoneal MΦs to secrete cytokines and release NO/ROS, even at concentrations as low as 12.5 μg ml−1. It was found that the O-specific polysaccharide moiety was more immunologically efficient than the glycolipid one, probably due to the position of 3-deoxy-d-manno-octulosonic acid. The results revealed effective structure–immunomodulating relationships of dLPS-derived moieties that are desirable in subcellular anti-cholera vaccine design.

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Immune responses to O-specific polysaccharide (OSP) in North American adults infected with Vibrio cholerae O1 Inaba

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0007874 ◽

2019 ◽

Vol 13 (11) ◽

pp. e0007874 ◽

Cited By ~ 1

Author(s):

Motaher Hossain ◽

Kamrul Islam ◽

Meagan Kelly ◽

Leslie M. Mayo Smith ◽

Richelle C. Charles ◽

...

Keyword(s):

Immune Responses ◽

Vibrio Cholerae ◽

North American ◽

Specific Polysaccharide ◽

Vibrio Cholerae O1

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Immunological efficacy of glycoconjugates derived from Vibrio cholerae O1 serotype Ogawa detoxified LPS in mice

Journal of Medical Microbiology ◽

10.1099/jmm.0.020875-0 ◽

2010 ◽

Vol 59 (12) ◽

pp. 1440-1448 ◽

Cited By ~ 7

Author(s):

Ema Paulovičová ◽

Jana Korcová ◽

Pavol Farkaš ◽

Slavomír Bystrický

Keyword(s):

B Cell ◽

Vibrio Cholerae ◽

Primary Vaccination ◽

Receptor Expression ◽

T Helper 1 ◽

Phagocytic Capacity ◽

Humoral And Cellular Immunity ◽

Significant Acceleration ◽

Specific Polysaccharide ◽

Vibrio Cholerae O1

This study focused on changes in selected parameters of humoral and cellular immunity following vaccination of mice with unique Vibrio cholerae LPS–protein-complexed conjugates. The V. cholerae detoxified LPS (dLPS)-derived antigenic structures O-specific polysaccharide (O-SP) and de-O-acylated LPS (DeOAc-LPS) were used to prepare glycoconjugates by linking both dLPSs to glucan, the immunomodulating matrix, and then to BSA carrier. Animals were given a primary vaccination and boosted at 2-week intervals with a dose of 4.5 μg saccharide antigen. The last boost was given either subcutaneously or intraperitoneally (i.p.) to compare the boosting effect and to optimize the effective immunization route. Both conjugates (O-SP–BSA and DeOAc-LPS–BSA) induced significant levels of antigen-specific Ig isotypes, especially IgG and IgM. The i.p. booster route was more effective. A T helper 1 response was achieved only by immunization with O-SP–BSA conjugate administered i.p. Significant acceleration of phagocytic capacity and respiratory burst of neutrophils was demonstrated by both immunogenic formulations. Activation of T- and B-cell adaptive immunities was exhibited as specific changes in CD3 : CD19 and CD4 : CD8 ratios, B-cell low-affinity Fcγ II and III receptor expression and induction of CD45R antigen.

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