scholarly journals Proteomic Identification of ADAM12 as a Regulator for TGF-β1-Induced Differentiation of Human Mesenchymal Stem Cells to Smooth Muscle Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e40820 ◽  
Author(s):  
Young Mi Kim ◽  
Jaeyoon Kim ◽  
Soon Chul Heo ◽  
Sang Hun Shin ◽  
Eun Kyoung Do ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Mesenchymal Stem Cells ◽  
Muscle Cells ◽  
Induced Differentiation ◽  
Proteomic Identification ◽  
Tgf Β1

MicroRNA-145-based differentiation of human mesenchymal stem cells to smooth muscle cells

2016 ◽  
Vol 38 (11) ◽  
pp. 1975-1981 ◽  
Author(s):  
Maryam Pajoohesh ◽  
Hossein Naderi-Manesh ◽  
Masoud Soleimani
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Mesenchymal Stem Cells ◽  
Muscle Cells

Evaluation of Mechanical Properties of Human Mesenchymal Stem Cells During Differentiation to Smooth Muscle Cells

2013 ◽  
Vol 42 (7) ◽  
pp. 1373-1380 ◽  
Author(s):  
Mohammad-Mehdi Khani ◽  
Mohammad Tafazzoli-Shadpour ◽  
Mostafa Rostami ◽  
Habibollah Peirovi ◽  
Mohsen Janmaleki
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Human Mesenchymal Stem Cells ◽  
Muscle Cells

scholarly journals Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells

2020 ◽  
Vol 10 (03) ◽  
pp. e335-e341
Author(s):  
Arunmani Mani ◽  
John W. Hotra ◽  
Sean C. Blackwell ◽  
Laura Goetzl ◽  
Jerrie S. Refuerzo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Preterm Birth ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uterine Smooth Muscle ◽  
Cell Based Therapy ◽  
Increased Risk

Abstract Objective The aim of this study was to determine if mesenchymal stem cells (MSCs) would suppress the inflammatory response in human uterine cells in an in vitro lipopolysaccharide (LPS)-based preterm birth (PTB) model. Study Design Cocultures of human uterine smooth muscle cells (HUtSMCs) and MSCs were exposed to 5 μg/mL LPS for 4 hours and further challenged with 1 μg/mL LPS for a subsequent 24 hours. Key elements of the parturition cascade regulated by toll-like receptors (TLRs) through activation of mitogen-activated protein kinases (MAPKs) were quantified in culture supernatant as biomarkers of MSC modulation. Results Coculture with MSCs significantly attenuated TLR-4, p-JNK, and p- extracellular signal-regulated kinase 1/2 (ERK1/2) protein levels compared with HUtSMCs monoculture (p = 0.05). In addition, coculture was associated with significant inhibition of proinflammatory cytokines interleukin (IL)-6 and IL-8 (p = 0.0001) and increased production of anti-inflammatory cytokines IL-10 and transforming growth factor (TGF)-β1 (p = 0.0001). Conclusion MSCs appear to play a role in significantly attenuating LPS-mediated inflammation via alteration of down-stream MAPKs. MSCs may represent a novel, cell-based therapy in women with increased risk of inflammatory-mediated preterm birth.

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