scholarly journals Evaluation of Multiple-Locus Variable Number of Tandem Repeats Analysis for Typing Livestock-Associated Methicillin-Resistant Staphylococcus aureus

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54425 ◽  
Author(s):  
Karin M. Brandt ◽  
Alexander Mellmann ◽  
Britta Ballhausen ◽  
Christian Jenke ◽  
Peter J. van der Wolf ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Tandem Repeats ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Variable Number ◽  
Methicillin Resistant ◽  
Multiple Locus

scholarly journals Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec type III

2011 ◽  
Vol 60 (4) ◽  
pp. 303-307 ◽  
Author(s):  
MOHAMMAD EMANEINI ◽  
LEILA JABALAMELI ◽  
HOSSEIN IMAN-EINI ◽  
MARZIEH ALIGHOLI ◽  
AMIR GHASEMI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Tandem Repeats ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Variable Number ◽  
Methicillin Resistant ◽  
Multiple Locus ◽  
Type Iii ◽  
Mrsa Strains ◽  
Type V

Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2% (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hld, found in 87.5% (126/144) of the isolates followed by lukE-lukD and hla found in 72.9% (105/144) and 70.1% (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3% (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5% (126/144) of the isolates. The rate of coexistence of hld with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7% (96/144), 44.4% (64/144) and 44.4% (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.

scholarly journals The Prevalence of Virulence Determinants and Antibiotic Resistance Patterns in Methicillin—Resistant Staphylococcus aureus in a Nursing Home in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 427
Author(s):  
Martyna Kasela ◽  
Agnieszka Grzegorczyk ◽  
Bożena Nowakowicz-Dębek ◽  
Anna Malm
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Variable Number Tandem Repeat ◽  
Multidrug Resistant ◽  
Variable Number ◽  
Pulse Field ◽  
Virulence Determinants ◽  
Gene Encoding ◽  
Methicillin Resistant ◽  
Pulse Field Gel Electrophoresis

Nursing homes (NH) contribute to the regional spread of methicillin-resistant Staphylococcus aureus (MRSA). Moreover, residents are vulnerable to the colonization and subsequent infection of MRSA etiology. We aimed at investigating the molecular and phenotypic characteristics of 21 MRSA collected from the residents and personnel in an NH (Lublin, Poland) during 2018. All MRSA were screened for 20 genes encoding virulence determinants (sea-see, eta, etb, tst, lukS-F-PV, eno, cna, ebpS, fib, bbp, fnbA, fnbB, icaADBC) and for resistance to 18 antimicrobials. To establish the relatedness and clonal complexes of MRSA in NH we applied multiple-locus variable-number tandem-repeat fingerprinting (MLVF), pulse field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. We identified four sequence types (ST) among two clonal complexes (CC): ST (CC22) known as EMRSA-15 as well as three novel STs—ST6295 (CC8), ST6293 (CC8) and ST6294. All tested MRSA were negative for sec, eta, etb, lukS-F-PV, bbp and ebpS genes. The most prevalent gene encoding toxin was sed (52.4%; n = 11/21), and adhesins were eno and fnbA (100%). Only 9.5% (n = 2/21) of MRSA were classified as multidrug-resistant. The emergence of novel MRSA with a unique virulence and the presence of epidemic clone EMRSA-15 creates challenges for controlling the spread of MRSA in NH.

scholarly journals Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia

Antibiotics ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 26 ◽  
Author(s):  
Jelena Asanin ◽  
Dusan Misic ◽  
Ksenija Aksentijevic ◽  
Zoran Tambur ◽  
Bojan Rakonjac ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Companion Animals ◽  
Antibiotic Resistance Genes ◽  
Variable Number ◽  
Animal Origin ◽  
Methicillin Resistant ◽  
Genetic Profiling ◽  
Biocide Resistance

The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates—30 obtained from humans and six from companion animals—were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL—Panton–Valentine leukocidin, ETs—exfoliative toxins, TSST—toxic shock syndrome toxin, SEs—staphylococcal enterotoxins, and MSCRAMMs—microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6′)-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and catpC221. All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.

scholarly journals Simplified protocol of gel-based multiple locus variable number of tandem repeats analysis (MLVA) for Staphylococcus aureus typing

GERMS ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 98-100 ◽  
Author(s):  
Hui-min Neoh ◽  
Choi-Ying Yieng ◽  
Raja Mohd Fadhil B Raja Abd Rahman ◽  
Salasawati Hussin ◽  
Rahman Jamal
Keyword(s):  
Staphylococcus Aureus ◽  
Tandem Repeats ◽  
Variable Number ◽  
Multiple Locus

scholarly journals Multiple-locus VNTR Analyses of Methicillin-resistant Staphylococcus aureus from Jamaica

2015 ◽  
Vol 8 ◽  
pp. IDRT.S31084 ◽  
Author(s):  
Paul D. Brown
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Chain Reaction ◽  
Methicillin Resistant ◽  
Multiple Locus ◽  
Type Iv ◽  
Constitutive Resistance ◽  
Polymerase Chain ◽  
Staphylococcal Cassette Chromosome

Background This study assessed the antimicrobial susceptibilities and the presence of inducible macrolide–lincosamide–streptogramin B (iMLSB) resistance in methicillin-resistant Staphylococcus aureus (MRSA) of Jamaica as well as the relatedness using polymerase chain reaction-based staphylococcal cassette chromosome mec (SCC mec) and multiple-locus variable numbers of tandem repeat analyses (MLVAs). Materials and Methods Antimicrobial susceptibility, the presence of MLSB resistance, and SCC mec and MLVA patterns were assessed for 61 nonduplicate isolates of MRSA from hospitalized patients. Results While no isolate was resistant to vancomycin, 53 (86.9%) isolates were resistant to ciprofloxacin, 52 (85.3%) to erythromycin, 49 (80%) to lincomycin, and 45 (74%) to clindamycin. Of the 52 erythromycin-resistant isolates, 48% exhibited constitutive resistance and 8% showed inducible MLSB (iMLSB) resistance. Most (85%) of typable isolates were SCC mec type IV, and among these, 16 MLVA patterns were identified. Conclusion Multidrug resistance continues to characterize MRSA. Among the erythromycin-resistant isolates, constitutive resistance and iMLSB resistance are common. These facts will complicate the treatment of MRSA infections and warrant continued surveillance and judicial use of antimicrobial agents.

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