scholarly journals Specific Insect-Virus Interactions Are Responsible for Variation in Competency of Different Thrips tabaci Isolines to Transmit Different Tomato Spotted Wilt Virus Isolates

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54567 ◽  
Author(s):  
Alana L. Jacobson ◽  
George G. Kennedy
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Thrips Tabaci ◽  
Insect Virus ◽  
Tomato Spotted Wilt ◽  
Virus Interactions ◽  
Wilt Virus ◽  
Virus Isolates

Some properties and thrip transmission of tomato spotted wilt virus in Canada

1974 ◽  
Vol 52 (6) ◽  
pp. 1177-1182 ◽  
Author(s):  
Y. C. Paliwal
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
High Titer ◽  
Tomato Leaf ◽  
Thrips Tabaci ◽  
Eastern Canada ◽  
Mesophyll Cells ◽  
Virus Particles ◽  
Tomato Spotted Wilt ◽  
Frankliniella Fusca ◽  
Wilt Virus

A virus isolated from white clover (Trifolium repens L.), dahlia, and tomato from Ontario, dahlia from Manitoba, and tomato and dahlia from British Columbia was identified as tomato spotted wilt virus (TSWV). Seven isolates of the virus examined had similar host reactions; four of them, investigated in detail, were similar in their localization in tomato leaf cells and physical properties also. The virus particles, 78–97 nm in diameter, were abundant in tomato leaf mesophyll cells and occurred in clusters or arrays surrounded by a membrane.The virus was purified by a method modified from a previously reported one and a specific antiserum was prepared. TSWV appears to be weakly immunogenic, as a high titer antiserum could not be obtained.At least three species of thrips, i.e. Thrips tabaci Lind., Frankliniella fusca Hinds, and F. occidentalis Perg., known as vectors of TSWV elsewhere, occur in Canada. F. fusca, which occurs in eastern Canada, efficiently transmitted all isolates of the virus. T. tabaci, a vector in several countries and widespread in Canada, failed to transmit the virus.

scholarly journals Tomato Spotted Wilt Virus Isolates in Japan are Grouped into Two Strains.

1993 ◽  
Vol 59 (6) ◽  
pp. 626-634 ◽  
Author(s):  
Shinya TSUDA ◽  
Kaoru HANADA ◽  
Yuzo MINOBE ◽  
Mitsuro KAMEYA-IWAKI ◽  
Keiichi TOMARU
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Virus Isolates

scholarly journals The Efficiency by Which Thrips tabaci Populations Transmit Tomato spotted wilt virus Depends on Their Host Preference and Reproductive Strategy

2002 ◽  
Vol 92 (6) ◽  
pp. 603-609 ◽  
Author(s):  
Elisavet K. Chatzivassiliou ◽  
Dick Peters ◽  
Nikolaos I. Katis
Keyword(s):  
Reproductive Strategy ◽  
Tomato Spotted Wilt Virus ◽  
Host Preference ◽  
Datura Stramonium ◽  
Thrips Tabaci ◽  
Transmission Parameters ◽  
Tomato Spotted Wilt ◽  
Access Period ◽  
Increasing Temperature ◽  
Wilt Virus

Arrhenotokous and thelytokous populations of Thrips tabaci from tobacco or leek plants were evaluated for their ability to transmit Tomato spotted wilt virus (TSWV) and for their host preference. Transmission efficiencies were comparatively studied using leaf disks of Petunia hybrida, Datura stramonium, and Nicotiana tabacum cv. Basmas. Adults of arrhenotokous populations collected on infected tobacco plants in the field were efficient transmitters (up to 48.5% transmission) and remained so when maintained on tobacco for several generations. Arrhenotokous T. tabacipopulations from leek plants were poor transmitters (up to 3.1% transmission), whereas no transmission was obtained with thelytokous populations from leek. All populations could infest leek, however none of the arrhenotokous and thelytokous populations from leek plants was able to infest tobacco. TSWV could be acquired by both first and second larval instars of a T. tabacipopulation from tobacco. However, the transmission by adults decreased with the age at which the virus was acquired by larvae. The highest efficiencies (61% of males and 51% of females transmitted) were obtained when newborn (0- to 24-h old) larvae acquired the virus. The majority of thrips started to transmit after becoming adult and rates were positively correlated with the temperature at which the thrips were kept. The median latent period values found for adults decreased with increasing temperature. The median acquisition access period (AAP50) of the population was 41 min, whereas the AAP50 was 65 min for males and 35 min for females. The median inoculation access period of males was 246 and 365 min on tobacco and petunia, respectively, and 96 and 345 min for females. The results show that T. tabaci forms a complex in terms of host preference, reproductive strategy, and ability to transmit TSWV. The transmission parameters show that the thrips of arrhenotokous populations infesting tobacco are highly efficient vectors.

scholarly journals Factors determining vector competence and specificity for transmission of Tomato spotted wilt virus

2002 ◽  
Vol 83 (3) ◽  
pp. 663-671 ◽  
Author(s):  
Tatsuya Nagata ◽  
Alice K. Inoue-Nagata ◽  
Jan van Lent ◽  
Rob Goldbach ◽  
Dick Peters
Keyword(s):  
Salivary Glands ◽  
Tomato Spotted Wilt Virus ◽  
Frankliniella Occidentalis ◽  
Vector Competence ◽  
Muscle Cells ◽  
Basal Membrane ◽  
Thrips Tabaci ◽  
Virus Particles ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

The competence of a Frankliniella occidentalis and a Thrips tabaci population to transmit Tomato spotted wilt virus (TSWV) was analysed. Adults of the F. occidentalis population transmitted this virus efficiently, whereas those of the thelytokous T. tabaci population failed to transmit. TSWV replicated in the midgut of the larvae of both populations after ingestion of virus; however, lower amounts accumulated in T. tabaci larvae than in F. occidentalis larvae. The virus was almost undetectable in T. tabaci adults, whereas high titres were readily detected in the F. occidentalis adults. The first infections in F. occidentalis larvae were detected by immunocytochemical studies in midgut epithelial and subsequently in midgut muscle cells, the ligaments, and finally in the salivary glands. The infections were weaker in the midgut epithelial and muscle cells of T. tabaci larvae, followed by an almost complete absence of any infection in the ligaments, and a complete absence in the salivary glands. Studies by electron microscopy revealed the budding of some virus particles from the basal membrane of midgut epithelial cells of F. occidentalis larvae into the extracellular space of the basal labyrinth. Enveloped virus particles were also seen in midgut muscle cells of F. occidentalis larvae. They were not discerned in epithelial and muscle cells of T. tabaci larvae and adults. This study showed that the rate of virus replication in the midgut and the extent of virus migration from the midgut to the visceral muscle cells and the salivary glands are probably crucial factors in the determination of vector competence.

scholarly journals First Report of Tomato Spotted Wilt Virus on Potatoes in Iran

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 442-442 ◽  
Author(s):  
R. Pourrahim ◽  
Sh. Farzadfar ◽  
A. A. Moini ◽  
N. Shahraeen ◽  
A. Ahoonmanesh
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Chenopodium Quinoa ◽  
Plant Viruses ◽  
Thrips Tabaci ◽  
Enzyme Linked Immunosorbent Assay ◽  
Leaf Extracts ◽  
Systemic Necrosis ◽  
First Report ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic chlorotic spots. TSWV was reported from ornamental crops in Tehran and Absard areas near to Firouzkoh province (2), but this is the first report of TSWV occurrence on potatoes in Iran. References: (1) T. S. Ie. Descriptions of Plant Viruses. No. 39, 1970. (2) A. A. Moeini, et al. Iran. J. Plant Pathol. (In press.)

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