scholarly journals Primary Neuron Culture for Nerve Growth and Axon Guidance Studies in Zebrafish (Danio rerio)

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57539 ◽  
Author(s):  
Zheyan Chen ◽  
Han Lee ◽  
Steven J. Henle ◽  
Thomas R. Cheever ◽  
Stephen C. Ekker ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Danio Rerio ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Nerve Growth ◽  
Primary Neuron Culture

Effects of hypoxic preconditioning on the expression of iron influx and efflux proteins in primary neuron culture

2012 ◽  
Vol 60 (4) ◽  
pp. 335-343 ◽  
Author(s):  
Fang Du ◽  
Ming Fan ◽  
Qi Gong ◽  
Ling Ling Zhu ◽  
Zhou-Jing Zhu ◽  
...  
Keyword(s):  
Hypoxic Preconditioning ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Primary Neuron Culture ◽  
Efflux Proteins

Neurosphere Development from Hippocampal and Cortical Embryonic Mixed Primary Neuron Culture: A Potential Platform for Screening Neurochemical Modulator

2018 ◽  
Vol 9 (11) ◽  
pp. 2870-2878 ◽  
Author(s):  
Juhee Khan ◽  
Gaurav Das ◽  
Varsha Gupta ◽  
Saswat Mohapatra ◽  
Subhajit Ghosh ◽  
...  
Keyword(s):  
Primary Neuron ◽  
Neuron Culture ◽  
Primary Neuron Culture

Toward a Merged Microfluidic/Microelectrode Array Device for Culture of Unidirectional Neural Network

2010 ◽  
Vol 1272 ◽  
Author(s):  
Alexander Mo ◽  
Sarah Heilshorn
Keyword(s):  
Neural Network ◽  
Soft Lithography ◽  
Microelectrode Array ◽  
Primary Neurons ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Initial Validation ◽  
Primary Neuron Culture ◽  
Neural Cultures ◽  
Two Populations

AbstractTraditional neural cultures are formed from disassociating primary neurons that are sourced from animals. However by disassociating them, any larger organizational structure between the neurons is lost. In an effort to regain some of the lost organization a device that is capable of recreating and monitoring a unidirectional connection between two populations is proposed. Initial validation toward a fully functional device is presented. Using soft lithography techniques, a microfluidic chip has been designed and produced with a design conducive for facilitating such a neuron culture. Using a specialized adhesive method, this chip can be attached and removed from a commercially available microelectrode array. Finally, cell viability is demonstrated using the PC12 neuron-like cell line after exploring several device configurations. Further efforts will be focused on primary neuron culture and establishment of a unidirectional network.

scholarly journals A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

10.3791/3823 ◽  
2012 ◽  
Author(s):  
Mariko Kobayashi ◽  
Ju-Youn Kim ◽  
Vladimir Camarena ◽  
Pamela C. Roehm ◽  
Moses V. Chao ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Culture System ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Virus Latency ◽  
Primary Neuron Culture ◽  
Simplex Virus

scholarly journals Spatiotemporally resolved subcellular phosphoproteomics

2021 ◽  
Vol 118 (25) ◽  
pp. e2025299118
Author(s):  
Yanjun Liu ◽  
Ruxin Zeng ◽  
Ruixuan Wang ◽  
Yicheng Weng ◽  
Ruixiang Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Underlying Mechanism ◽  
Living Cells ◽  
Light Illumination ◽  
Primary Neuron ◽  
Quantitative Mass Spectrometry ◽  
Neuron Culture ◽  
Biotin Ligase ◽  
Primary Neuron Culture ◽  
Rapid Activation

Proteome-wide profiling of protein phosphorylation has been widely used to reveal the underlying mechanism of diverse cellular signaling events. Yet, characterizing subcellular phosphoproteome with high spatial–temporal resolution has remained challenging. Herein, we developed a subcellular-specific uncaging-assisted biotinylation and mapping of phosphoproteome (SubMAPP) strategy to monitor the phosphorylation dynamics of subcellular proteome in living cells and animals. Our method capitalizes on the genetically encoded bioorthogonal decaging strategy, which enables the rapid activation of subcellular localized proximity labeling biotin ligase through either light illumination or small-molecule triggers. By further adopting an integrated orthogonal pull-down strategy with quantitative mass spectrometry, SubMAPP allowed for the investigation of subcellular phosphoproteome dynamics, revealing the altered phosphorylation patterns of endoplasmic reticulum (ER) luminal proteins under ER stress. Finally, we further expanded the scope of the SubMAPP strategy to primary neuron culture and living mice.

Sign in / Sign up

Export Citation Format

Share Document