scholarly journals Neuroglobin-overexpression alters hypoxic response gene expression in primary neuron culture following oxygen glucose deprivation

Neuroscience ◽  
2009 ◽  
Vol 162 (2) ◽  
pp. 396-403 ◽  
Author(s):  
Z. Yu ◽  
J. Liu ◽  
S. Guo ◽  
C. Xing ◽  
X. Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Primary Neuron ◽  
Response Gene ◽  
Hypoxic Response ◽  
Neuron Culture ◽  
Primary Neuron Culture

Effects of hypoxic preconditioning on the expression of iron influx and efflux proteins in primary neuron culture

2012 ◽  
Vol 60 (4) ◽  
pp. 335-343 ◽  
Author(s):  
Fang Du ◽  
Ming Fan ◽  
Qi Gong ◽  
Ling Ling Zhu ◽  
Zhou-Jing Zhu ◽  
...  
Keyword(s):  
Hypoxic Preconditioning ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Primary Neuron Culture ◽  
Efflux Proteins

scholarly journals Primary Neuron Culture for Nerve Growth and Axon Guidance Studies in Zebrafish (Danio rerio)

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57539 ◽  
Author(s):  
Zheyan Chen ◽  
Han Lee ◽  
Steven J. Henle ◽  
Thomas R. Cheever ◽  
Stephen C. Ekker ◽  
...  
Keyword(s):  
Axon Guidance ◽  
Danio Rerio ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Nerve Growth ◽  
Primary Neuron Culture

Neurosphere Development from Hippocampal and Cortical Embryonic Mixed Primary Neuron Culture: A Potential Platform for Screening Neurochemical Modulator

2018 ◽  
Vol 9 (11) ◽  
pp. 2870-2878 ◽  
Author(s):  
Juhee Khan ◽  
Gaurav Das ◽  
Varsha Gupta ◽  
Saswat Mohapatra ◽  
Subhajit Ghosh ◽  
...  
Keyword(s):  
Primary Neuron ◽  
Neuron Culture ◽  
Primary Neuron Culture

Toward a Merged Microfluidic/Microelectrode Array Device for Culture of Unidirectional Neural Network

2010 ◽  
Vol 1272 ◽  
Author(s):  
Alexander Mo ◽  
Sarah Heilshorn
Keyword(s):  
Neural Network ◽  
Soft Lithography ◽  
Microelectrode Array ◽  
Primary Neurons ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Initial Validation ◽  
Primary Neuron Culture ◽  
Neural Cultures ◽  
Two Populations

AbstractTraditional neural cultures are formed from disassociating primary neurons that are sourced from animals. However by disassociating them, any larger organizational structure between the neurons is lost. In an effort to regain some of the lost organization a device that is capable of recreating and monitoring a unidirectional connection between two populations is proposed. Initial validation toward a fully functional device is presented. Using soft lithography techniques, a microfluidic chip has been designed and produced with a design conducive for facilitating such a neuron culture. Using a specialized adhesive method, this chip can be attached and removed from a commercially available microelectrode array. Finally, cell viability is demonstrated using the PC12 neuron-like cell line after exploring several device configurations. Further efforts will be focused on primary neuron culture and establishment of a unidirectional network.

scholarly journals A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

10.3791/3823 ◽  
2012 ◽  
Author(s):  
Mariko Kobayashi ◽  
Ju-Youn Kim ◽  
Vladimir Camarena ◽  
Pamela C. Roehm ◽  
Moses V. Chao ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Culture System ◽  
Primary Neuron ◽  
Neuron Culture ◽  
Virus Latency ◽  
Primary Neuron Culture ◽  
Simplex Virus

scholarly journals Gene Expression Profiles of Human Cerebral Organoids Identify PPAR Pathway and PKM2 as Key Markers for Oxygen-Glucose Deprivation and Reoxygenation

2021 ◽  
Vol 15 ◽  
Author(s):  
Naoki Iwasa ◽  
Takeshi K. Matsui ◽  
Naohiko Iguchi ◽  
Kaoru Kinugawa ◽  
Naritaka Morikawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Stroke ◽  
Signaling Pathway ◽  
Expression Profiles ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Ppar Signaling ◽  
The Impact

Ischemic stroke is one of the most common neurological diseases. However, the impact of ischemic stroke on human cerebral tissue remains largely unknown due to a lack of ischemic human brain samples. In this study, we applied cerebral organoids derived from human induced pluripotent stem cells to evaluate the effect of oxygen-glucose deprivation/reoxygenation (OGD/R). Pathway analysis showed the relationships between vitamin digestion and absorption, fat digestion and absorption, peroxisome proliferator-activated receptor (PPAR) signaling pathway, and complement and coagulation cascades. Combinational verification with transcriptome and gene expression analysis of different cell types revealed fatty acids-related PPAR signaling pathway and pyruvate kinase isoform M2 (PKM2) as key markers of neuronal cells in response to OGD/R. These findings suggest that, although there remain some limitations to be improved, our ischemic stroke model using human cerebral organoids would be a potentially useful tool when combined with other conventional two-dimensional (2D) mono-culture systems.

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