Porous Tantalum Coatings Prepared by Vacuum Plasma Spraying Enhance BMSCs Osteogenic Differentiation and Bone Regeneration In Vitro and In Vivo

Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.

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Development and evaluation of the innovative 3D printed scaffolds for bone regeneration in vitro and in vivo

10.15388/vu.thesis.262 ◽

2021 ◽

Author(s):

◽

Ieva Gendvilienė

Keyword(s):

Bone Regeneration ◽

3D Printed ◽

Regeneration In Vitro

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In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering

BioMed Research International ◽

10.1155/2017/6434169 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Beom Su Kim ◽

Feride Shkembi ◽

Jun Lee

Keyword(s):

Bone Regeneration ◽

Osteogenic Differentiation ◽

Release Kinetics ◽

Human Mesenchymal Stem Cells ◽

Calvarial Defect ◽

Defect Model ◽

Fibrin Gel ◽

In Vivo Evaluation

Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10−7–10−4 M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young’s modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.

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Characterization of HA/Ta Composite Coatings Fabricated by Vacuum Plasma Spraying

Materials Science Forum ◽

10.4028/www.scientific.net/msf.761.113 ◽

2013 ◽

Vol 761 ◽

pp. 113-116 ◽

Cited By ~ 1

Author(s):

Li Ping Huang ◽

You Tao Xie ◽

Heng Ji ◽

Yi Zeng ◽

Xue Bin Zheng

Keyword(s):

Plasma Spraying ◽

Bonding Strength ◽

Composite Coatings ◽

X Ray Diffraction ◽

Vacuum Plasma Spraying ◽

Vacuum Plasma ◽

Plasma Sprayed ◽

Bonelike Apatite

Plasma sprayed hydroxyapatite (HA) coatings on titanium alloy substrates have been used extensively due to their excellent biocompatibility and osteoconductivity. However, the low bonding strength between HA and Ti substrates is still problematic in the long-term implantation lifespan. In this paper, HA/Ta composite coatings with various Ta contents (20% and 60%) were fabricated by vacuum plasma spraying (VPS). The microstructure, phase composition of the coatings was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively. The results revealed that the coatings had a rough surface and lamellar structure including some pores. ASTM C-633 standard was used for the measurement of bonding strength, which was found increasing with the increase of Ta contents in the composite coatings. The bonding strength of the composite coating containing 60% Ta (H4T6) reached to 37.2 MPa, which is about 1.9 times that of HA coating. In vitro bioactivity evaluated in simulated body fluids (SBF) showed that bonelike apatite layer was formed on the composite coatings surface, which indicated the good bioactivity of the HA/Ta composite coatings.

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