Purification and Characterization of a Novel and Robust L-Asparaginase Having Low-Glutaminase Activity from Bacillus licheniformis: In Vitro Evaluation of Anti-Cancerous Properties

L-asparaginase from bacteria has been used in treatment of acute lymphoblastic leukemia. The aim of this study was to purify and characterize L-asparaginase fromPhaseolus vulgarisseeds instead of microbial sources. L-asparaginase was purified to apparent homogeneity. The enzyme has molecular mass of 79 kDa. The purified asparaginase had very low activity toward a number of asparagine and glutamine analogues. L-asparaginase was free from glutaminase activity. Kinetic parameters, Km andVmax of purified enzyme, were found to be 6.72 mM and 0.16 μM, respectively. The enzyme had optimum pH at 8.0. The enzyme showed high stability at alkaline pH (pH 7.5–9.0) when incubated for up to 24 h. L-asparaginase had the same temperature optimum and thermal stability at 37°C. K+was able to greatly enhance the activity of asparaginase by 150% compared with other metals tested. In conclusion, L-asparaginase showed no glutaminase activity and good stability over a wide range of physiological conditions, and thus it could be used as a potential candidate for treatment of acute lymphoblastic leukemia.

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Synthesis and characterization of a new aroylhydrazone ligand and its cobalt(III) complexes: X-ray crystallography and in vitro evaluation of antibacterial and antifungal activities

Journal of Molecular Structure ◽

10.1016/j.molstruc.2018.10.061 ◽

2019 ◽

Vol 1178 ◽

pp. 544-553 ◽

Cited By ~ 13

Author(s):

Nimya Ann Mathews ◽

Anitha Jose ◽

M.R. Prathapachandra Kurup

Keyword(s):

Synthesis And Characterization ◽

In Vitro Evaluation ◽

Antifungal Activities ◽

X Ray ◽

X Ray Crystallography ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

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Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

Food Science & Nutrition ◽

10.1002/fsn3.1416 ◽

2020 ◽

Vol 8 (2) ◽

pp. 1272-1283 ◽

Cited By ~ 1

Author(s):

Vignesh Samayanpaulraj ◽

Muthukumar Sivaramapillai ◽

Sankara Naynar Palani ◽

Krishnaveni Govindaraj ◽

Vijay Velu ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

In Vitro Evaluation ◽

Channa Striata ◽

Virulent Aeromonas Hydrophila ◽

Identification And Characterization

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Characterization of genipin-crosslinked gelatin/hyaluronic acid-based hydrogel membranes and loaded with hinokitiol: In vitro evaluation of antibacterial activity and biocompatibility

Materials Science and Engineering C ◽

10.1016/j.msec.2019.110074 ◽

2019 ◽

Vol 105 ◽

pp. 110074 ◽

Cited By ~ 6

Author(s):

Kai-Chi Chang ◽

Dan-Jae Lin ◽

Yu-Ren Wu ◽

Chin-Wei Chang ◽

Chih-Hua Chen ◽

...

Keyword(s):

Antibacterial Activity ◽

Hyaluronic Acid ◽

In Vitro Evaluation ◽

Hydrogel Membranes

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Purification and characterization of a peptide from Bacillus licheniformis showing dual antimicrobial and emulsifying activities

Food Research International ◽

10.1016/j.foodres.2008.08.010 ◽

2009 ◽

Vol 42 (1) ◽

pp. 63-68 ◽

Cited By ~ 21

Author(s):

Mário Lettieri Teixeira ◽

Florencia Cladera-Olivera ◽

Juliana dos Santos ◽

Adriano Brandelli

Keyword(s):

Bacillus Licheniformis ◽

Purification And Characterization

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Characterization of a new TiF4 and β-cyclodextrin inclusion complex and its in vitro evaluation on inhibiting enamel demineralization

Archives of Oral Biology ◽

10.1016/j.archoralbio.2012.11.001 ◽

2013 ◽

Vol 58 (3) ◽

pp. 239-247 ◽

Cited By ~ 21

Author(s):

Camila Nassur ◽

Adílis Kalina Alexandria ◽

Luciana Pomarico ◽

Valeria Pereira de Sousa ◽

Lúcio Mendes Cabral ◽

...

Keyword(s):

Inclusion Complex ◽

In Vitro Evaluation ◽

Enamel Demineralization ◽

Cyclodextrin Inclusion ◽

Cyclodextrin Inclusion Complex

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Protamine messenger RNA: partial purification and characterization of a heterogeneous family of polyadenylated messenger components

Canadian Journal of Biochemistry ◽

10.1139/o79-115 ◽

1979 ◽

Vol 57 (6) ◽

pp. 945-958 ◽

Cited By ~ 8

Author(s):

Kostas Iatrou ◽

Lashitew Gedamu ◽

Gordon H. Dixon

Keyword(s):

Nucleotide Sequence ◽

Messenger Rna ◽

Rnase H ◽

Partial Purification ◽

Polyacrylamide Gels ◽

Purification And Characterization ◽

Sequence Variations ◽

Duplex Formation

Poly(A)+ protamine mRNA (pmRNA) components were isolated after separation on denaturing preparative polyacrylamide gels. The four size classes of protamine mRNA described previously were found to contain poly(A) tracts of different lengths. The pmRNA1 was found to be associated with (A)110, pmRNA2 with (A)90, pmRNA3 with (A)85, and pmRNA4 with (A)69. Following deadenylation with RNase H after duplex formation with oligo-dT, the isolated mRNAs were found to be still heterogeneous, although highly enriched in certain of the deadenylated components. DNA complementary to the isolated mRNAs (cDNA) was synthesized in vitro. Following depurination, the oligopyrimidine maps indicated that C7T4, corresponding to an Arg-Arg-Gly-Gly sequence in protamine and originally thought to be characteristic of all mRNA components, is present in only one or possibly two of the components. Cross-hybridizations between the cDNAs and the four poly(A)+ pmRNAs indicated that a basic polynucleotide unit of substantial length is common to all four mRNAs and that the existing nucleotide sequence variations probably originate from one or both of the non-coding portions of the mRNA molecules.

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