Comprehensive Evolutionary and Expression Analysis of FCS-Like Zinc finger Gene Family Yields Insights into Their Origin, Expansion and Divergence

Stress-associated proteins (SAPs) are a class of zinc finger proteins that confer tolerance to a variety of abiotic and biotic stresses in diverse plant species. However, in cucumber (Cucumis sativus L.), very little is known about the roles of SAP gene family members in regulating plant growth, development, and stress responses. In this study, a total of 12 SAP genes (named as CsSAP1-CsSAP12) were identified in the cucumber genome, which were unevenly distributed on six chromosomes. Gene duplication analysis detected one tandem duplication and two segmental duplication events. Phylogenetic analysis of SAP proteins from cucumber and other plants suggested that they could be divided into seven groups (sub-families), and proteins in the same group generally had the same arrangement of AN1 (ZnF-AN1) and A20 (ZnF-A20) domains. Most of the CsSAP genes were intronless and harbored a number of stress- and hormone-responsive cis-elements in their promoter regions. Tissue expression analysis showed that the CsSAP genes had a broad spectrum of expression in different tissues, and some of them displayed remarkable alteration in expression during fruit development. RT-qPCR results indicated that all the selected CsSAP genes displayed transcriptional responses to cold, drought, and salt stresses. These results enable the first comprehensive description of the SAP gene family in cucumber and lay a solid foundation for future research on the biological functions of CsSAP genes.

Download Full-text

Isolation and expression analysis of a human zinc finger gene (ZNF41) located on the short arm of the X chromosome

Genomics ◽

10.1016/0888-7543(91)90367-n ◽

1991 ◽

Vol 9 (4) ◽

pp. 728-736 ◽

Cited By ~ 9

Author(s):

Annamaria Franzè ◽

Nicoletta Archidiacono ◽

Mariano Rocchi ◽

Maria Marino ◽

Giovanna Grimaldi

Keyword(s):

Zinc Finger ◽

Expression Analysis ◽

X Chromosome ◽

Zinc Finger Gene

Download Full-text

miR-1279, miR-548j, miR-548m, and miR-548d-5p Binding Sites in CDSs of Paralogous and OrthologousPTPN12,MSH6, andZEB1Genes

BioMed Research International ◽

10.1155/2013/902467 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 8

Author(s):

Anatoliy T. Ivashchenko ◽

Assel S. Issabekova ◽

Olga A. Berillo

Keyword(s):

Gene Family ◽

Mismatch Repair ◽

Zinc Finger ◽

Binding Sites ◽

Animal Species ◽

Tyrosine Phosphatase ◽

Nucleotide Sequences ◽

Orthologous Genes ◽

Zinc Finger Gene ◽

Dna Mismatch

OnlyPTPN12, MSH6, andZEB1have significant miR-1279 binding sites among paralogous genes of human tyrosine phosphatase family, DNA mismatch repair family, and zinc finger family, respectively. All miRNA binding sites are located within CDSs of studied mRNAs. Nucleotide sequences of hsa-miR-1279 binding sites with mRNAs of humanPTPN12, MSH6, andZEB1genes encode TKEQYE, EGSSDE, and GEKPYE oligopeptides, respectively. The conservation of miRNA binding sites encoding oligopeptides has been revealed. MRNAs of many paralogs of zinc finger gene family have from 1 to 12 binding sites coding the same GEKPYE hexapeptide. MRNAs ofPTPN12, MSH6, andZEB1orthologous genes from different animal species have binding sites for hsa-miR-1279 which consist of homologous oligonucleotides encoding similar human oligopeptides TKEQYE, EGSSDE, and GEKPYE. MiR-548j, miR-548m, and miR-548d-5p have homologous binding sites in the mRNA ofPTPN12orthologous genes which encode PRTRSC, TEATDI, and STASAT oligopeptides, respectively. All regions of miRNA are important for binding with the mRNA.

Download Full-text