scholarly journals Transcriptome Analysis of Blunt Snout Bream (Megalobrama amblycephala) Reveals Putative Differential Expression Genes Related to Growth and Hypoxia

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142801 ◽  
Author(s):  
Fu-Gui Li ◽  
Jie Chen ◽  
Xia-Yun Jiang ◽  
Shu-Ming Zou
Keyword(s):  
Differential Expression ◽  
Transcriptome Analysis ◽  
Megalobrama Amblycephala ◽  
Blunt Snout Bream ◽  
Differential Expression Genes

scholarly journals Transcriptome Analysis and SSR/SNP Markers Information of the Blunt Snout Bream (Megalobrama amblycephala)

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e42637 ◽  
Author(s):  
Zexia Gao ◽  
Wei Luo ◽  
Hong Liu ◽  
Cong Zeng ◽  
Xiaolian Liu ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Snp Markers ◽  
Megalobrama Amblycephala ◽  
Blunt Snout Bream

scholarly journals Transcriptome Analysis Reveals Sexual Disparities between Olfactory and Immune Gene Expression in the Olfactory Epithelium of Megalobrama amblycephala

2021 ◽  
Vol 22 (23) ◽  
pp. 13017
Author(s):  
Maolin Lv ◽  
Xiuli Chen ◽  
Xin Huang ◽  
Ning Liu ◽  
Weimin Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Olfactory Epithelium ◽  
Transcriptome Analysis ◽  
Olfactory Organ ◽  
Megalobrama Amblycephala ◽  
Fish Analysis ◽  
Immune Gene ◽  
Blunt Snout Bream ◽  
Receptor Cells ◽  
Males And Females

The olfactory organ is an important chemoreceptor in vertebrates. However, the sexual disparities in gene expression patterns in the olfactory organ in fish remain unstudied. Here, we conducted a transcriptome analysis of the olfactory epithelium (OE) of male and female blunt snout bream (Megalobrama amblycephala) to identify the differences. The histological analysis showed that there were 22 leaf-like olfactory lamellaes on one side of the OE of the adult blunt snout bream. The sensory area of OE is enriched with ciliated receptor cells and microvilli receptor cells. The transcriptome analysis showed that only 10 out of 336 olfactory receptor genes (224 ORs, 5 V1Rs, 55 V2Rs, and 52 TAARs) exhibited significant expression differences between males and females, and most of the differentially expressed genes were related to the immune system. We also validated these results using qPCR: 10 OR genes and 6 immunity-related genes significantly differed between males and females. The FISH analysis results indicated that the ORs were mainly expressed at the edge of the olfactory lamellae. Collectively, our study reveals that gender is not an important factor influencing the expression of olfactory receptors, but the expression of immune genes varies greatly between the genders in blunt snout bream.

Screening, cloning and sequence analysis of the differential expression genes in Longissimus dorsi of Yanbian Yellow Cattle

2011 ◽  
Vol 33 (11) ◽  
pp. 1219-1224 ◽  
Author(s):  
Wan-Nian TIAN ◽  
Shou-Fa ZHANG ◽  
Xiang-Zi LI ◽  
Qing-Shan GAO ◽  
Xin JIN ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Differential Expression ◽  
Longissimus Dorsi ◽  
Yellow Cattle ◽  
Differential Expression Genes

scholarly journals Molecular Characterization of PGC-1β (PPAR Gamma Coactivator 1β) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala)

2020 ◽  
Vol 21 (6) ◽  
pp. 1935 ◽  
Author(s):  
Kangle Lu ◽  
Tomas Policar ◽  
Xiaojun Song ◽  
Samad Rahimnejad
Keyword(s):  
Molecular Characterization ◽  
Mitochondrial Biogenesis ◽  
Phylogenetic Analyses ◽  
Ppar Gamma ◽  
Megalobrama Amblycephala ◽  
Fat Intake ◽  
High Fat ◽  
Blunt Snout Bream ◽  
Mitochondria Biogenesis

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1β (PGC-1β) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1β was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1β family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1β in fish. The function of PGC-1β was evaluated through overexpression and knockdown of PGC-1β in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1β along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1β did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1β. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1β induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1β is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1β/NRF-1 pathway.

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