scholarly journals Validation of quantitative real-time PCR reference genes for the determination of seasonal and labor-specific gene expression profiles in the head of Western honey bee, Apis mellifera

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0200369 ◽  
Author(s):  
KyungHwan Moon ◽  
Si Hyeock Lee ◽  
Young Ho Kim
Keyword(s):  
Gene Expression ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Specific Gene ◽  
Quantitative Real Time Pcr

scholarly journals Selection and Validation of Reference Genes for Quantitative Real-Time PCR Expression Analysis of Candidate Genes in Carposina sasakii (Lepidoptera: Carposinidae)

2020 ◽  
pp. 75-85
Author(s):  
Zuobing Yan ◽  
Yongli Li ◽  
Zhou Zhou ◽  
Yongan Zhang ◽  
Liangjian Qu
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Analysis ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Quantitative Real Time Pcr ◽  
Carposina Sasakii

Carposina sasakii is one of the most important pests on the quality of stone and pome fruits. Investigation of a gene expression level in the species is hampered because of the gap of validated reference genes. The expression variation in the transcription levels of eight candidate reference genes, Actin (ACT), Tubulinbeta-1 (TUB), Ribosomal protein 49 (RP49), Elongation factor1-alpha (EF-1a), Elongation factor1-b (EF-1b), Elongation factor1-d (EF-1d), Ribosomal proteinL13 (RPL13) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were analyzed by quantitative real-time PCR (qPCR). The stability and ranking of these gene expression profiles in three organ types (head, thorax and abdomen), three developmental stages (larva, pupa and moth), and five diapause states (non-diapause, pre-diapause, diapause 0 d, diapause 20 d and diapause 60 d) were assessed using two algorithm-based methods, geNorm and NormFinder. EF-1a, ACT and GAPDH were evaluated to be the three stable reference genes based on the important observations and comprehensive analysis, whereas TUB and EF-1b showed low expression stability. Best gene combinations for different qPCR analysis in C. sasakii could be chosen from the three stable reference genes, the using of two reference genes is sufficient to effectively normalize qPCR data in C. sasakii. The study laid the foundation for gene expression analysis in C. sasakii and provided new information for the selection of reference genes.

Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection

2020 ◽  
Author(s):  
X. W. Ke ◽  
L. H. Yin ◽  
J. Xu ◽  
W. N. Sun ◽  
X. D. Xu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Mung Bean ◽  
Expression Profiles ◽  
Pcr Analysis ◽  
Saline Stress ◽  
Stable Gene ◽  
Quantitative Real Time Pcr

The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-á was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.

Determination of the panel of reference genes for quantitative real-time PCR in fetal and adult rat intestines

2021 ◽  
Author(s):  
Xiaoqian Lu ◽  
Yi Liu ◽  
Dingmei Zhang ◽  
Kexin Liu ◽  
Qian Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Adult Rat
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