Susceptibility of monomicrobial or polymicrobial biofilms derived from infected diabetic foot ulcers to topical or systemic antibiotics in vitro

Diabetic foot ulcers are one of the most dreadful complications of diabetes mellitus and efforts to accelerate diabetic wound healing are of paramount importance to prevent ulcer infections and subsequent lower-limb amputations. There are several treatment approaches for the management of diabetic foot ulcers and honey seems to be a safe and cost-effective therapeutic approach on top of standard of care. The aim of this review was to summarize the therapeutic properties of honey and the data regarding its possible favorable effects on diabetic wound healing. A literature search of articles from 1986 until April 2021 was performed using MEDLINE, EMBASE, and the Cochrane Library to assess for studies examining the therapeutic wound healing properties of honey, it's in vitro effect, and the efficacy and/or mechanism of action of several types of honey used for the treatment of diabetic animal wounds. Honey has antioxidant, anti-inflammatory, and antibacterial properties and in vitro studies of keratinocytes and fibroblasts, as well as studies in diabetic animal models show that treatment with honey is associated with increased re-epithelialization and collagen production, higher wound contraction, and faster wound healing. The use of honey could be a promising approach for the management of diabetic foot ulcers.

Download Full-text

In vitro bacteriologic study and empiric antibiotic regimens for diabetic foot ulcers

African Journal of Microbiology Research ◽

10.5897/ajmr11.728 ◽

2012 ◽

Vol 6 (27) ◽

Cited By ~ 1

Author(s):

Ishrat Bano,

Keyword(s):

Diabetic Foot ◽

Foot Ulcers ◽

Diabetic Foot Ulcers ◽

Empiric Antibiotic

Download Full-text

Efektivitas Kompres Iodine Terhadap Zona Hambat Staphylococcus Aureus Pada Ulkus Diabetikum

Jurnal Keperawatan Indonesia ◽

10.7454/jki.v16i3.323 ◽

2013 ◽

Vol 16 (3) ◽

pp. 139-144

Author(s):

Asep Kuswandi ◽

Kusmiyati Kusmiyati ◽

Holikin Holikin

Keyword(s):

Staphylococcus Aureus ◽

Diabetic Foot ◽

Wound Care ◽

Positive Culture ◽

Foot Ulcers ◽

Distilled Water ◽

Diabetic Foot Ulcers ◽

Average Size

AbstrakUlkus diabetikum merupakan salah satu komplikasi kronis diabetes melitus dan menjadi penyebab amputasi kaki. Infeksi pada ulkus tersebut umumnya disebabkan oleh Staphylococcus aureus. Salah satu teknik pencegahan amputasi kaki diabetes adalah perawatan ulkus. Berbagai jenis bahan kompres ulkus diabetikum yang telah dikenal selama ini adalah:kompres madu, gula, Iodine, dan NaCl 0,9%. Penelitian ini bertujuan mengetahui efektifitas bahan kompres ulkus terhadap daya hambat Staphylococcus aureus. Penelitian ini merupakan penelitian eksperimen in vitro menggunakan satu faktor perlakuan yaitu zona hambat bahan kompres terhadap Staphylococcus aureus pada ulkus diabetikum. Enam jenis bahan yang diuji adalah aquadest, gula, Iodine 10%, campuran gula dan Iodine 10 %, madu, dan NaCl 0,9 %. Eksperimen dilakukan sebanyak 10 kali untuk mengetahui rerata luas daya hambatnya selama 24 jam. Sampel diambil dari ulkus diabetikum. Staphylococcus aureus diisolasi dari ulkus tersebut untuk eksperimen dengan berbagai bahan kompres ulkus. Hasil penelitian menunjukkan bahwa zona hambat madu rata-rata sebesar 0,4 mm, gula 3,0 mm, aquadest 0,0 mm, NaCl 0,9% 0,0 mm, Iodine 10% 8,3 mm dan campuran gula-Iodine 6,0 mm. Zona hambat terluas dihasilkan dari uji menggunakkan Iodine 10% (8,3 mm). Sementara campuran gula Iodine 10% menghasilkan zona hambat seluas 6 mm dan gula menghasilkan 3 mm. Penelitian ini merekomendasikan jika ditemukan Staphylococcus aureus maka dilakukan kompres Iodine 10% dengan kasa steril. Penelitian selanjutnya dengan metode in vivo perlu dilakukan untuk mengetahui efektifitas cairan Iodine pada ulkus diabetikum.Kata Kunci: kompres ulkus, staphylococcus aureus, ulkus diabetikum, zona hambatAbstractEffectivity of Iodine Compress to Blocking Zone of Staphylococcus aureus in Diabetes Ulcers. Diabetic foot ulcers is one of diabetes chronic complications that might lead to leg amputations. Staphylococcus aureus is known as the cause of infection in diabetic foot ulcers. One of techniques to prevent diabetic foot amputations is wound care. Various materials are known to be used to compress diabetic foot ulcers. These include of using honey, sugar, 10% of Iodine and 0.9% of NaCl. This study aimed ttify to identify the effectiveness of various wound care materials to block Staphylococcus aureus. This is an in vitro experiment study to investigate the effetiveness of six wound care materials used to wounds compress: distilled water, sugar, 10% of Iodine, a mixture of sugar and Iodine, honey and 0.9% of NaCl. Experiments were carried out in 10 times to determine the average size of block area in 24 hours. Staphylococcus aureus were isolated and soiled with various wound compressss materialls. The results showed that honey produced 0.4 mm of a blocking zone, 3.0 mm for glucose, 0.0 mm for distilled water, 0.0 mm for 0.9% of NaCl, 8.3 mm for 10% of Iodine, and 6.0 mm for mixed-Iodine Sugar. Ten percents of Iodine produced the widest zone to block Staphylicoccus aureus. This study recommends of apllying a 10% of Iodine compress if there is a positive culture of Staphylococcus aureus. A further in vivo study is a necessity to investigate the effectiveness of Iodine to diabetes foot ulcers.Keywords: blocking zone, diabetic foot ulcers, Staphylococcus aureus, wound compress

Download Full-text

Platelet lysate promotes the healing of long-standing diabetic foot ulcers: A report of two cases and in vitro study

Heliyon ◽

10.1016/j.heliyon.2020.e03929 ◽

2020 ◽

Vol 6 (5) ◽

pp. e03929

Author(s):

Hanan Jafar ◽

Maram Hasan ◽

Dana Al-Hattab ◽

Mohanad Saleh ◽

Lena Abu Ameereh ◽

...

Keyword(s):

Diabetic Foot ◽

In Vitro Study ◽

Platelet Lysate ◽

Vitro Study ◽

Foot Ulcers ◽

Diabetic Foot Ulcers

Download Full-text

Not Only Antimicrobial: Metronidazole Mitigates the Virulence of Proteus mirabilis Isolated from Macerated Diabetic Foot Ulcer

Applied Sciences ◽

10.3390/app11156847 ◽

2021 ◽

Vol 11 (15) ◽

pp. 6847

Author(s):

Ahdab N. Khayyat ◽

Hisham A. Abbas ◽

Mamdouh F. A. Mohamed ◽

Hani Z. Asfour ◽

Maan T. Khayat ◽

...

Keyword(s):

Virulence Factors ◽

Proteus Mirabilis ◽

Diabetic Foot ◽

Biofilm Formation ◽

Foot Ulcer ◽

Diabetic Foot Ulcer ◽

Foot Ulcers ◽

Resistant Bacteria ◽

Diabetic Foot Ulcers

Diabetic foot ulcers are recognized to be a severe complication of diabetes, increasing the risk of amputation and death. The bacterial infection of Diabetic foot ulcers with virulent and resistant bacteria as Proteus mirabilis greatly worsens the wound and may not be treated with conventional therapeutics. Developing new approaches to target bacterial virulence can be helpful to conquer such infections. In the current work, we evaluated the anti-virulence activities of the widely used antibacterial metronidazole. The minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentrations (MEBC) were determined for selected antibiotics which P. mirabilis was resistant to them in the presence and absence of metronidazole in sub-MIC. The effect of metronidazole in sub-MIC on P. mirabilis virulence factors as production of exoenzymes, motilities, adhesion and biofilm formation, were evaluated. Furthermore, molecular docking of metronidazole into P. mirabilis adhesion and essential quorum sensing (QS) proteins, was performed. The results revealed a significant ability of metronidazole to in-vitro inhibit P. mirabilis virulence factors and antagonize its essential proteins. Moreover, metronidazole markedly decreased the MICs and MBECs of tested antibiotics. Conclusively, metronidazole in sub-MIC is a plausible anti-virulence and anti-QS agent that can be combined to other antibiotics as anti-virulence adjuvant to defeat aggressive infections.

Download Full-text