Effect of host fruit, temperature and Wolbachia infection on survival and development of Ceratitis capitata immature stages

AbstractFruit flies (Diptera: Tephritidae) are the most damaging pests on fruit crops on Réunion Island, near Madagascar. Survival and development of the Mediterranean fruit fly, Ceratitiscapitata (Wiedemann), the Natal fruit fly, C. rosa Karsch and the Mascarenes fruit fly, C. catoirii Guérin-Mèneville were compared at five constant temperatures spanning 15 to 35°C. Durations of the immature stages of C. capitata, C. rosa and C catoirii ranged from 14.5–63.8, 18.8–65.7 and 16.8–65.8 days, respectively, at 30–15°C. The lower developmental threshold and thermal constant were calculated using the temperature summation model. The thermal constant for total development of the immature stages of C. capitata, C. rosa and C. catoirii were 260, 405 and 356 DD, respectively. Species differed mainly during the larval stages and ovarian maturation period, with smaller differences in the egg stage. Ceratitis rosa appeared to be better adapted to low temperatures than the two other species as it showed a lower larval developmental threshold of 3.1°C compared to 10.2°C for C. capitata and 8.9°C for C. catoirii. Overall, C. catoirii had a low survival rate within the range of temperatures studied. The different responses of the three Ceratitis species to various temperatures explain to some extent their distribution on the island. The results obtained will be used for optimizing laboratory rearing procedures and for constructing computer simulation models to predict fruit fly population dynamics.

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Nonhost Status of Commercial Sweet Granadilla (Passiflora ligularis) in Peru to Ceratitis capitata (Diptera: Tephritidae) and Anastrepha fraterculus

Journal of Economic Entomology ◽

10.1093/jee/toaa026 ◽

2020 ◽

Vol 113 (3) ◽

pp. 1158-1175

Author(s):

Orlando S Dolores ◽

Javier M Layme ◽

Carlos C Huaynate

Keyword(s):

Development Time ◽

Ceratitis Capitata ◽

Oviposition Behavior ◽

Fruit Fly ◽

Resistance Mechanisms ◽

Natural Host ◽

Host Fruit ◽

Anastrepha Fraterculus ◽

Fruit Maturity ◽

Host Status

Abstract The host status of sweet granadilla (Passifflora ligularis Juss.) to Ceratitis capitata (Wiedemann) and Anastrepha fraterculus (Wiedemann) in Peru was determined. Experiments were conducted in Pasco (Peru) in four different orchards, over 2 yr (2016 and 2017), two orchards per year. Choice (granadilla plus natural host) and no-choice foraging behavior trials were conducted using sleeves under field conditions, and forced infestation was examined in laboratory cages, with five females per fruit. The development time of C. capitata was determined, and the oviposition behavior of C. capitata and A. fraterculus was examined. Three fruit maturity stages of intact (n = 1,320) and punctured (n = 1,320) granadilla fruits were examined. Adult C. capitata (n = 4,418) and A. fraterculus (n = 2,484) were trapped in the orchards, and commercial granadilla fruits (n = 1,940) sampled and dissected. Fruit fly infestation was not found in any intact granadilla fruits. Larvae and pupae were found inside punctured granadilla only in fruits broken after 20 d, and adults only emerged when those pupae were removed from the fruit. Ceratitis capitata development time was longer in punctured granadilla than that in host fruit. In the oviposition test, A. fraterculus and C. capitata did not lay eggs in intact granadilla, and C. capitata laid eggs in punctured fruits but larvae were not found. Because of the resistance mechanisms of the pericarp, commercial fruits of Passiflora ligularis are not a natural host of C. capitata and A. fraterculus in Peru.

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Photosensitizing Effect of Hematoporphyrin IX on Immature Stages of Ceratitis capitata (Diptera: Tephritidae)

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.2009.00704.x ◽

2010 ◽

Vol 86 (3) ◽

pp. 639-644 ◽

Cited By ~ 7

Author(s):

Luciana Mercedes Pujol-Lereis ◽

Ana Massaldi ◽

Alejandro Rabossi ◽

Luis Alberto Quesada-Allué

Keyword(s):

Ceratitis Capitata ◽

Immature Stages ◽

Hematoporphyrin Ix

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Asymmetries in Physiological State as a Possible Cause of Resident Advantage in Contests

Behaviour ◽

10.1163/156853998792913546 ◽

1998 ◽

Vol 135 (8) ◽

pp. 1013-1030 ◽

Cited By ~ 19

Author(s):

Russell Messing ◽

Daniel Papaj

Keyword(s):

Ceratitis Capitata ◽

Physiological State ◽

Egg Laying ◽

Field Cage ◽

Searching Behaviour ◽

Relative Age ◽

State Variable ◽

Dynamical State ◽

Host Fruit ◽

Possible Cause

AbstractAs is common in defense of resources in many animals, contests on host fruit between female Mediterranean fruit flies (Ceratitis capitata) are generally resolved in favour of the resident individual. Here we offer an interpretation of resident advantage in this species which is derived from a dynamical state-variable perspective on behaviour. We first demonstrated the occurrence of residence advantage. In field-cage assays of freely-foraging and freely-interacting females within a tree bearing host coffee berries, the occurrence of two females on a berry almost always resulted in contests. Approximately half of the contests among females on berries resulted in clear winners; resident females won an overwhelming majority of such contests. Contests tended to occur while the resident fly was currently engaged in egg-laying, either boring into the berry with their ovipositor or marking the berry after laying eggs. Non-residents, by contrast, were uniformly engaged in searching behaviour. We next tested the hypothesis that degree of resident advantage is a function of the degree to which a resident is engaged in egg-laying behaviour just prior to the contest. In experimental manipulations of resident status, which female won depended strongly on what residents were doing at the time contests were initiated. If residents were resting and grooming, non-residents (who were uniformly searching) usually won. If residents were laying eggs or marking the fruit after laying eggs, residents usually won. Only when both residents and non-residents were engaged in searching behaviour did the outcome of a contest not depend on resident status. Finally, we tested the hypothesis that contest outcome is related to the dynamical state of the contestants in terms of their relative age. We staged contests between host-deprived females that differed in age by two weeks, manipulating age independently of resident status. Older females won virtually all contests with younger ones regardless of resident status. In a field-cage assay of freely-foraging flies, older females also engaged in significantly more egg-laying activity. Taken together, these results indicate that older females value a fruit more highly than do younger ones, invest more in defense of that resource and therefore win more contests.

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The Host Fruit Amplifies Mutualistic Interactions between Ceratitis Capitata Larvae and Associated Bacteria

10.1101/327668 ◽

2018 ◽

Author(s):

Doron Shalom Yishai Zaada ◽

Michael Ben-Yosef ◽

Boaz Yuval ◽

Edouard Jurkevitch

Keyword(s):

Larval Development ◽

Ceratitis Capitata ◽

Fruit Fly ◽

Bacterial Strains ◽

Negative Effects ◽

Host Fruit ◽

Major Pest ◽

Fly Larvae ◽

The Given ◽

First Time

AbstractBackground:The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval development, very little is known about the interaction between bacteria and larvae in their true ecological context. Understanding their relationship and inter-dependence in the host fruit is important for the development of new pest control interfaces to deal with this pest.Results:We find no negative effects on egg hatch or larval development brought about by the bacterial isolates tested. The various symbionts inhabiting the fly’s digestive system differ in their degree of contribution to the development of fly larvae depending on the given host and their sensitivity to induced inhibition caused by female produced antimicrobial peptides. These differences were observed not only at the genus or species level but also between isolates of the same species. We demonstrate how the microbiota from the mother’s gut supports the development of larvae in the fruit host and show that larvae play a major role in spreading the bacterial contagion in the infected fruit itself. In addition, we present (for the first time) evidence for horizontal transfer of bacteria between larvae of different maternal origin that develop together in the same fruit.Conclusions:Larvae play a major role in the spread and shaping of the microbial population in the fruit. The transfer of bacteria between different individuals developing in the same fruit suggests that the infested fruit serves as a microbial hub for the amplification and spread of bacterial strains between individuals.

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