scholarly journals Extracellular vesicles from rat-bone-marrow mesenchymal stromal/stem cells improve tendon repair in rat Achilles tendon injury model in dose-dependent manner: A pilot study

PLoS ONE ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. e0229914 ◽  
Author(s):  
Clarissa Gissi ◽  
Annalisa Radeghieri ◽  
Cristina Antonetti Lamorgese Passeri ◽  
Marialucia Gallorini ◽  
Lucia Calciano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Pilot Study ◽  
Tendon Repair ◽  
Tendon Injury ◽  
Dependent Manner ◽  
Injury Model ◽  
Dose Dependent ◽  
Stromal Stem Cells ◽  
Rat Bone

Effect of rat bone marrow stromal stem cells overexpressing platelet-derived growth factor and bone morphogenic protein into platelet-rich plasma gels on osteogenic differentiation

2020 ◽  
Vol 10 (7) ◽  
pp. 1068-1078
Author(s):  
Jin Sun ◽  
Xin Jiang ◽  
Weilian Chen ◽  
Weikun Zheng ◽  
Junhao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Growth Factor ◽  
Osteogenic Differentiation ◽  
Platelet Rich Plasma ◽  
Genetically Modified ◽  
Bone Morphogenic Protein ◽  
Platelet Derived Growth Factor ◽  
Stromal Stem Cells ◽  
Rat Bone

The aim of this study was to improve osteoblast function by incorporating rat bone marrow stromal stem cells (rBMSCs) overexpressing platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) into platelet-rich plasma (PRP) gels. rBMSCs were isolated, cultured, and identified. The rBMSCs were subsequently co-transfected with two recombinant adenoviruses delivering PDGF-BB-GFP and BMP-2-GFP. PDGF-BB and BMP-2 expression levels in transduced BMSCs were detected, and a post-transfection analysis of the osteogenic differentiation trend of rBMSCs was performed. Autologous PRP gels were constructed and optimized, and the levels of growth factor in PRP were detected. The optimal growth conditions of the genetically-modified rBMSCs in the scaffolds were established, and the effects of tissue engineering materials and PRP gel construction on the osteogenic differentiation of rBMSCs were assessed. The results revealed that high-purity rBMSCs were obtained, and high levels of BMP-2 and PDGF-BB were secreted by the transduced cells. Furthermore, PRP promoted the proliferation and osteogenic differentiation of rBMSCs overexpressing PDGF-BB and BMP-2. Collectively, the results of the present study revealed that genetically modified rBMSCs incorporated into PRP gels enhanced osteogenic differentiation.

scholarly journals Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROS/p53/p21Cip1/Waf1Pathway

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Mingyu Zhang ◽  
Yue Du ◽  
Renzhong Lu ◽  
You Shu ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cellular Senescence ◽  
Dependent Manner ◽  
Cycle Arrest ◽  
Marrow Mesenchymal Stem Cells ◽  
Dose Dependent ◽  
Increased Activity

In the present study, we demonstrated that bone marrow mesenchymal stem cells (BMSCs) of the 3rd passage displayed the senescence-associated phenotypes characterized with increased activity of SA-β-gal, altered autophagy, and increased G1 cell cycle arrest, ROS production, and expression of p53 andp21Cip1/Waf1compared with BMSCs of the 1st passage. Cholesterol (CH) reduced the number of SA-β-gal positive cells in a dose-dependent manner in aging BMSCs induced by H2O2and the 3rd passage BMSCs. Moreover, CH inhibited the production of ROS and expression of p53 andp21Cip1/Waf1in both cellular senescence models and decreased the percentage of BMSCs in G1 cell cycle in the 3rd passage BMSCs. CH prevented the increase in SA-β-gal positive cells induced by RITA (reactivation of p53 and induction of tumor cell apoptosis, a p53 activator) or 3-MA (3-methyladenine, an autophagy inhibitor). Our results indicate that CH not only is a structural component of cell membrane but also functionally contributes to regulating cellular senescence by modulating cell cycle, autophagy, and the ROS/p53/p21Cip1/Waf1signaling pathway.

Sodium Butyrate Pre-Treatment Enhance Differentiation of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) into Hepatocytes and Improve Liver Injury

2021 ◽  
Vol 21 ◽  
Author(s):  
Qiu-Yun Li ◽  
Juan Chen ◽  
Yong-Heng Luo ◽  
Wei Zhang ◽  
En-Hua Xiao
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dependent Manner ◽  
Hepatic Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Specific Factors ◽  
Pre Treatment ◽  
Dose Dependent

Objective: The treatment of liver failure by stem cell transplantation has attracted growing interest. Herein, we aim to explore the role of sodium butyrate (NaB) in the hepatic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) under liver-specific factors induction in vitro and vivo. Materials & Methods: We isolated BM-MSCs from the mononuclear cell fraction of rabbit bone marrow samples, and identified the cells by Immunophenotypic analysis. We investigated the effects of different concentrations and induction conditions. The histone deacetylase inhibitor NaB induced hepatic differentiation of BM-MSCs under liver-specific factors induction in vitro. Morphological features, liver-specific gene and protein expression, and functional analyses in vitro and vivo were performed to evaluate the hepatic differentiation of BM-MSCs. Results: Our results showed that pre-treated NaB inhibited the expression of liver-specific protein in a dose-dependent manner. The induction efficiency of NaB with 24h pre-treatment was higher than that of NaB continuous intervention. 0.5 mM 24h NaB pre-treated cells can improve liver tissue damage in vivo. And the liver ALB, AAT and the serum TP were significantly increased, while the serum ALT was significantly reduced. Conclusion: Continuous NaB treatment can inhibit BM-MSCs proliferation in a dose-dependent manner at a certain concentration range. 0.5 mM 24h pre-treatment of NaB enhanced differentiation of BM-MSCs into hepatocytes and improves liver injury in vitro and vivo.

p75NTR suppression in rat bone marrow stromal stem cells significantly reduced their rate of apoptosis during neural differentiation

2011 ◽  
Vol 498 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Houri Edalat ◽  
Zahra Hajebrahimi ◽  
Mansoureh Movahedin ◽  
Mahmoud Tavallaei ◽  
Sara Amiri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Neural Differentiation ◽  
Stromal Stem Cells ◽  
Rat Bone

Bone Marrow–Derived Mesenchymal Stem Cells Promote Angiogenic Processes in a Time- and Dose-Dependent Manner In Vitro

2009 ◽  
Vol 15 (9) ◽  
pp. 2459-2470 ◽  
Author(s):  
Garry P. Duffy ◽  
Tabassum Ahsan ◽  
Timothy O'Brien ◽  
Frank Barry ◽  
Robert M. Nerem
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dependent Manner ◽  
Dose Dependent

scholarly journals Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

2019 ◽  
Vol 12 (6) ◽  
pp. 273-279 ◽  
Author(s):  
Jirun Apinun ◽  
Sittisak Honsawek ◽  
Somsak Kuptniratsaikul ◽  
Jutarat Jamkratoke ◽  
Sorada Kanokpanont
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Pilot Study ◽  
Osteogenic Differentiation ◽  
Collagen Hydrogel ◽  
Collagen Hydrogels ◽  
Rat Bone

AbstractBackgroundSilk fibroin (SF) can be processed into a hydrogel. SF/collagen hydrogel may be a suitable biomaterial for bone tissue engineering.ObjectivesTo investigate in vitro biocompatibility and osteogenic potential of encapsulated rat bone marrow-derived mesenchymal stem cells (rat MSCs) in an injectable Thai SF/collagen hydrogel induced by oleic acid–poloxamer 188 surfactant mixture in an in vitro pilot study.MethodsRat MSCs were encapsulated in 3 groups of hydrogel scaffolds (SF, SF with 0.05% collagen [SF/0.05C], and SF with 0.1% collagen [SF/0.1C]) and cultured in a growth medium and an osteogenic induction medium. DNA, alkaline phosphatase (ALP) activity, and calcium were assayed at periodically for up to 5 weeks. After 6 weeks of culture the cells were analyzed by scanning electron microscopy and energy dispersive spectroscopy.ResultsAlthough SF hydrogel with collagen seems to have less efficiency to encapsulate rat MSCs, their plateau phase growth in all hydrogels was comparable. Inability to maintain cell viability as cell populations declined over 1–5 days was observed. Cell numbers then plateaued and were maintained until day 14 of culture. ALP activity and calcium content of rat MSCs in SF/collagen hydrogels were highest at day 21. An enhancing effect of collagen combined with the hydrogel was observed for proliferation and matrix formation; however, benefits of the combination on osteogenic differentiation and biomineralization are as yet unclear.ConclusionRat MSCs in SF and SF/collagen hydrogels showed osteogenic differentiation. Accordingly, these hydrogels may serve as promising scaffolds for bone tissue engineering.

Proprotein convertases 1 and 2 (PC1 and PC2) are expressed in neurally differentiated rat bone marrow stromal stem cells (BMSCs)

2007 ◽  
Vol 420 (3) ◽  
pp. 198-203 ◽  
Author(s):  
Mohammad Marandi ◽  
Seyed Javad Mowla ◽  
Mahmoud Tavallaei ◽  
Mohammad Mehdi Yaghoobi ◽  
Seyed Mehdi Jafarnejad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Proprotein Convertases ◽  
Stromal Stem Cells ◽  
Rat Bone
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