scholarly journals Decomposition and organic amendments chemistry explain contrasting effects on plant growth promotion and suppression of Rhizoctonia solani damping off

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0230925
Author(s):  
Giuliano Bonanomi ◽  
Maurizio Zotti ◽  
Mohamed Idbella ◽  
Nice Di Silverio ◽  
Linda Carrino ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rhizoctonia Solani ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Organic Amendments ◽  
Damping Off

scholarly journals Influence of Bacillus Spp. Based Bioproducts on Potato Plant Growth and Control of Rhizoctonia Solani

2019 ◽  
Vol 76 (1) ◽  
pp. 29
Author(s):  
Sorina DINU ◽  
Oana Alina BOIU-SICUIA ◽  
Florica CONSTANTINESCU
Keyword(s):  
Plant Growth ◽  
Rhizoctonia Solani ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Aqueous Suspension ◽  
Potato Plants ◽  
Bacillus Spp ◽  
Seeding Material ◽  
Biocontrol Potential ◽  
And Control

Some Bacillus based bioproducts were analyzed for their plant growth promotion and Rhizoctonia solani biocontrol potential in potato plants. The bioproducts were formulated as concentrated aqueous suspension, each containing one of the following plant beneficial bacteria: Bacillus safensis Rd.b2, Bacillus spp. 75.1s and Cp.b4 strains. These were applied on potato seeding material in order to evaluate plant growth promotion effects. The biocontrol efficacy was also evaluated, using Rhizoctonia solani DSM 63002 as plant pathogen, and Prestige 290FS as reference chemical treatment.In the plant growth-promotion experiments, several biologic parameters were biometrically evaluated. Best results regarding plant growth and vigor were obtained using CropMax, a commercial phytostimulatory product. However, the bacterial treatment with Bacillus spp. Cp.b4 and 75.1s showed an improved plant growth compared to the untreated control. An efficacy of 93.75% against Rhizoctonia dumping-off was registered when using the Prestige 290FS chemical control. Mix treatments based on this pesticide, in low dose, combined with Cp.b4 or 75.1s biocontrol strains significantly reduced the pathogenic attack, showing 85 to 87.5% efficacy.The present research demonstrated that the bacterial bioproducts based on Bacillus spp. 75.1s and Cp.b4 strains increase plant growth and are highly effective in controlling Rhizoctonia attack in potato plants.

scholarly journals Biological Control of Chili Damping-Off Disease, Caused by Pythium myriotylum

2021 ◽  
Vol 12 ◽  
Author(s):  
Sajjad Hyder ◽  
Amjad Shahzad Gondal ◽  
Zarrin Fatima Rizvi ◽  
Rashida Atiq ◽  
Muhammad Irtaza Sajjad Haider ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Root Weight ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Pythium Myriotylum ◽  
Pgp Traits

Pythium myriotylum is a notorious soil-borne oomycete that causes post-emergence damping-off in chili pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is an interesting strategy. The present study was performed to isolate and characterize PGPR indigenous to the chili rhizosphere in Pakistan, and to test the potential to suppress the damping-off and plant growth promotion in chili. Out of a total of 28 antagonists, eight bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C, and RH-87) significantly suppressed the colony growth of P. myriotylum in a dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus, and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4–39.0 μg mL–1), solubilized inorganic phosphate (75–103 μg mL–1), and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varying levels of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found to be non-pathogenic to chili seeds and notably enhanced percentage seed germination, plumule, redical length, and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes such as Peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) activates. Moreover, the treatment of chili seeds with these bacterial isolates significantly suppressed the damping-off caused by P. myriotylum and improved PGP traits compared to the control. In addition, a positive correlation was noticed between shoot, root length, and dry shoot and root weight, and there was a negative correlation between dry shoot, root weight, and seedling percentage mortality. These results showed that native PGPR possesses multiple traits beneficial to the chili plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

scholarly journals Biological control of chili damping off disease, caused by Pythium myriotylum

2020 ◽  
Author(s):  
Sajjad Hyder ◽  
Amjad Shahzad Gondal ◽  
Zarrin Fatima Rizvi ◽  
Muhammad Irtaza Sajjad Haider ◽  
Muhammad Inam-ul-Haq
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Root Weight ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Pythium Myriotylum ◽  
Pgp Traits

ABSTRACTPythium myriotylum is a notorious soil-borne oomycete causes post-emergence damping off in chilli pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is eye catching strategy. The present study was performed to isolate and characterize PGPR indigenous to chili rhizosphere in Pakistan, and to test their potential to suppress damping off and plant growth promotion in chilli. Out of total 28 antagonists, 8 bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C and RH-87) significantly suppressed the colony growth of P. myriotylum in dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4-39.0 μg ml−1), solubilized inorganic phosphate (75–103 μgml-1) and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varied level of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found non-pathogenic to chill seeds and notably enhanced percentage seed germination, plumule, redical length and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes (PO, PPO and PAL) activates. Moreover, chilli seeds treatment with these bacterial isolates significantly suppressed damping-off caused by P. myriotylum, and improved PGP traits as compared to control. In addition, a positive correlation was noticed between shoot, root length and dry shoot and root weigh and a negative correlation was seen between dry shoot, root weight and seedling percentage mortality. These results showed that native PGPR possess multiple traits beneficial to the chilli plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

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