scholarly journals Genomic diversity of 39 samples of Pyropia species grown in Japan

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252207
Author(s):  
Yukio Nagano ◽  
Kei Kimura ◽  
Genta Kobayashi ◽  
Yoshio Kawamura
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Parental Species ◽  
Genetic Relationships ◽  
Genomic Diversity ◽  
Organellar Genome ◽  
Organellar Genomes ◽  
Chloroplast Dna Sequences ◽  
Nuclear Genomes ◽  
Whole Genome Resequencing

Some Pyropia species, such as nori (P. yezoensis), are important marine crops. We conducted a phylogenetic analysis of 39 samples of Pyropia species grown in Japan using organellar genome sequences. A comparison of the chloroplast DNA sequences with those from China showed a clear genetic separation between Japanese and Chinese P. yezoensis. Conversely, comparing the mitochondrial DNA sequences did not separate Japanese and Chinese P. yezoensis. Analysis of organellar genomes showed that the genetic diversity of Japanese P. yezoensis used in this study is lower than that of Chinese wild P. yezoensis. To analyze the genetic relationships between samples of Japanese Pyropia, we used whole-genome resequencing to analyze their nuclear genomes. In the offspring resulting from cross-breeding between P. yezoensis and P. tenera, nearly 90% of the genotypes analyzed by mapping were explained by the presence of different chromosomes originating from two different parental species. Although the genetic diversity of Japanese P. yezoensis is low, analysis of nuclear genomes genetically separated each sample. Samples isolated from the sea were often genetically similar to those being farmed. Study of genetic heterogeneity of samples within a single aquaculture strain of P. yezoensis showed that samples were divided into two groups and the samples with frequent abnormal budding formed a single, genetically similar group. The results of this study will be useful for breeding and the conservation of Pyropia species.

scholarly journals Whole-genome SNP analysis elucidates the genetic population structure and diversity of Acrocomia species

2020 ◽  
Author(s):  
Brenda G. Díaz ◽  
Maria I. Zucchi ◽  
Alessandro. Alves-Pereira ◽  
Caléo P. de Almeida ◽  
Aline C. L. Moraes ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Structure ◽  
Genetic Relationships ◽  
Geographical Area ◽  
Taxonomic Classification ◽  
Genomic Diversity ◽  
Productive Capacity ◽  
Snp Analysis ◽  
Genetic Population

AbstractAcrocomia (Arecaceae) is a genus widely distributed in tropical and subtropical America that has been achieving economic interest due to the great potential of oil production of some of its species. In particular A. aculeata, due to its vocation to supply oil with the same productive capacity as the oil palm even in areas with water deficit. Although eight species are recognized in the genus, the taxonomic classification based on morphology and geographic distribution is still controversial. Knowledge about the genetic diversity and population structure of the species is limited, which has limited the understanding of the genetic relationships and the orientation of management, conservation, and genetic improvement activities of species of the genus. In the present study, we analyzed the genomic diversity and population structure of seven species of Acrocomia including 117 samples of A. aculeata covering a wide geographical area of occurrence, using single nucleotide Polymorphism (SNP) markers originated from Genotyping By Sequencing (GBS). The genetic structure of the Acrocomia species were partially congruent with the current taxonomic classification based on morphological characters, recovering the separation of the species A. aculeata, A. totai, A. crispa and A. intumescens as distinct taxonomic groups. However, the species A. media was attributed to the cluster of A. aculeata while A. hassleri and A. glauscescens were grouped together with A. totai. The species that showed the highest and lowest genetic diversity were A. totai and A. media, respectively. When analyzed separately, the species A. aculeata showed a strong genetic structure, forming two genetic groups, the first represented mainly by genotypes from Brazil and the second by accessions from Central and North American countries. Greater genetic diversity was found in Brazil when compared to the other countries. Our results on the genetic diversity of the genus are unprecedented, as is also establishes new insights on the genomic relationships between Acrocomia species. It is also the first study to provide a more global view of the genomic diversity of A. aculeata. We also highlight the applicability of genomic data as a reference for future studies on genetic diversity, taxonomy, evolution and phylogeny of the Acrocomia genus, as well as to support strategies for the conservation, exploration and breeding of Acrocomia species and in particular A. aculeata.

scholarly journals Genetic Diversity of Paeonia rockii (Flare Tree Peony) Germplasm Accessions Revealed by Phenotypic Traits, EST-SSR Markers and Chloroplast DNA Sequences

Forests ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 672
Author(s):  
Xin Guo ◽  
Fangyun Cheng ◽  
Yuan Zhong
Keyword(s):  
Genetic Diversity ◽  
Chloroplast Dna ◽  
Ssr Markers ◽  
Dna Sequences ◽  
Genetic Background ◽  
Background Information ◽  
Phenotypic Traits ◽  
Tree Peony ◽  
Chloroplast Dna Sequences ◽  
Paeonia Rockii

Research Highlights: This study, based on the first collection of cultivated Paeonia rockii (flare tree peony, FTP) germplasm across the main distribution area by our breeding desires, comprehensively evaluates these accessions by using phenotypic traits, expressed sequence tag (EST)-simple sequence repeat (SSR) markers and chloroplast DNA sequences (cpDNA). The results show that these accessions collected selectively by us can represent the genetic background information of FTP as a germplasm of tree crops. Background and Objectives: FTP has high cultural, ornamental and medicinal value traditionally, as well as recently presenting a significance as an emerging edible oil with high α-linolenic acid contents in the seeds. The objectives of this study are to reveal the characteristics of the genetic diversity of FTP, as well as to provide scientific suggestions for the utilization of tree peony breeding and the conservation of germplasm resource. Materials and Methods: Based on the phenotypic traits, EST-SSR markers and chloroplast DNA sequence variation, we studied the diversity of a newly established population of 282 FTP accessions that were collected and propagated by ourselves in our breeding project in recent years. Results: (1) There was an abundant variation in phenotype of the accessions, and the phenotypic variation was evenly distributed within the population, without significant hierarchical structure, (2) the EST-SSR data showed that these 282 accessions had relatively high genetic diversity, in which a total of 185 alleles were detected in 34 pairs of primers. The 282 accessions were divided into three distinct groups, and (3) the chloroplast DNA sequences (cpDNA) data indicated that these accessions had a higher genetic diversity than the population level and a lower genetic diversity than the species level of wild P. rockii, and the existing spatial genetic structure of these accessions can be divided into two branches. Conclusions: From the results of the three analyses, we found that these accessions can fully reflect the genetic background information of FTP germplasm resources, so their protection and utilization will be of great significance for genetic improvement of woody peonies.

Molecular phylogeny of the genus Hordeum using three chloroplast DNA sequences

Genome ◽  
2002 ◽  
Vol 45 (6) ◽  
pp. 1157-1166 ◽  
Author(s):  
Tomotaro Nishikawa ◽  
Björn Salomon ◽  
Takao Komatsuda ◽  
Roland von Bothmer ◽  
Koh-ichi Kadowaki
Keyword(s):  
Chloroplast Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Genetic Relationships ◽  
Closely Related Species ◽  
Dna Phylogeny ◽  
Molecular Phylogenetic ◽  
Hordeum Jubatum ◽  
Chloroplast Dna Sequences ◽  
Chloroplast Dna Regions

The genus Hordeum consists of three cytotypes (2x, 4x, and 6x). Its reproductive isolation has been incomplete between closely related species and hence the genetic relationship is reticulate and complex. We used 32 taxa of Hordeum and three chloroplast DNA sequences, matK, atpB–rbcL, and trnL–trnF, in the current study. Molecular phylogenetic analysis based on sequence data of the three chloroplast DNA regions clearly demonstrated genetic relationships among taxa and origin of polypoids. The formation of H. secalinum likely involved hybridization between Hordeum marinum subsp. marinum and a Eurasian diploid possessing the H genome. The formation of hexaploid Hordeum brachyantherum involved hybridization between tetraploid H. brachyantherum and diploid H. marinum subsp. gussoneanum. The formation of three tetraploids, H. brachyantherum, Hordeum jubatum, and Hordeum guatemalense, probably involved hybridization between H. brachyantherum subsp. californicum and an altered H genome diploid. The formation of Hordeum arizonicum involved the two taxa Hordeum pusillum and H. jubatum.Key words: chloroplast DNA, phylogeny, Hordeum, barley.

Assessment of genomic diversity among wheat genotypes as determined by simple sequence repeats

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 646-651 ◽  
Author(s):  
M Ahmad
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Simple Sequence Repeats ◽  
Common Ancestor ◽  
Genetic Relationships ◽  
Genomic Diversity ◽  
Pedigree Information ◽  
Wheat Genotypes ◽  
Wide Range ◽  
Simple Sequence

Simple sequence repeats (SSRs) have been used to examine the genomic diversity of wheat (Triticum aestivum L.) germplasm. Thirteen wheat genotypes of diverse origin were analyzed with 43 selected SSRs to provide uniform and maximum genome coverage. A total of 156 allelic variants were detected at 43 SSR loci, ranging from two to eight per locus with an average of 3.6. The polymorphic information content (PIC) values of the loci ranged from 0.10 (Xgwm264) to 0.89 (Xgwm471 and Xgwm577). Genetic similarities calculated from SSR data ranged from 30.1 ('Era' and 'Klasic') to 90.1 ('Neepawa' and 'Thatcher') between genotypes. UPGMA analysis based on genetic distance estimates produced three loose groupings that were generally consistent with available pedigree information. Cultivars 'Neepawa' and 'Thatcher' are closely related. Their genetic relationship was confirmed by the facts that they share a common ancestor and are clustered together. There were two different 'Era' genotypes, one used in the 'Otane' pedigree and one used in this study. None of the other genotypes had a close common ancestor indicating any close genetic relationships. Principal coordinate analysis also confirmed this pattern of genetic diversity. A wide range of genomic diversity was observed among all the genotypes, proving them to be prime candidates for selective breeding for specific traits and broadening the genetic base.Key words: simple sequence repeats, genetic diversity, Triticum aestivum, genetic similarity estimates, cluster analysis.

scholarly journals Genome-wide SNP analysis to assess the genetic population structure and diversity of Acrocomia species

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0241025
Author(s):  
Brenda Gabriela Díaz ◽  
Maria Imaculada Zucchi ◽  
Alessandro Alves‐Pereira ◽  
Caléo Panhoca de Almeida ◽  
Aline Costa Lima Moraes ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Structure ◽  
Genetic Relationships ◽  
Geographical Area ◽  
Taxonomic Classification ◽  
Genomic Diversity ◽  
Productive Capacity ◽  
Snp Analysis ◽  
Genetic Population

Acrocomia (Arecaceae) is a genus widely distributed in tropical and subtropical America that has been achieving economic interest due to the great potential of oil production of some of its species. In particular A. aculeata, due to its vocation to supply oil with the same productive capacity as the oil palm (Elaeis guineenses) even in areas with water deficit. Although eight species are recognized in the genus, the taxonomic classification based on morphology and geographic distribution is still controversial. Knowledge about the genetic diversity and population structure of the species is limited, which has limited the understanding of the genetic relationships and the orientation of management, conservation, and genetic improvement activities of species of the genus. In the present study, we analyzed the genomic diversity and population structure of Acrocomia genus, including 172 samples from seven species, with a focus on A. aculeata with 117 samples covering a wide geographical area of occurrence of the species, using Single Nucleotide Polymorphism (SNP) markers originated from Genotyping By Sequencing (GBS).The genetic structure of the Acrocomia species were partially congruent with the current taxonomic classification based on morphological characters, recovering the separation of the species A. aculeata, A. totai, A. crispa and A. intumescens as distinct taxonomic groups. However, the species A. media was attributed to the cluster of A. aculeata while A. hassleri and A. glauscescens were grouped together with A. totai. The species that showed the highest and lowest genetic diversity were A. totai and A. media, respectively. When analyzed separately, the species A. aculeata showed a strong genetic structure, forming two genetic groups, the first represented mainly by genotypes from Brazil and the second by accessions from Central and North American countries. Greater genetic diversity was found in Brazil when compared to the other countries. Our results on the genetic diversity of the genus are unprecedented, as is also establishes new insights on the genomic relationships between Acrocomia species. It is also the first study to provide a more global view of the genomic diversity of A. aculeata. We also highlight the applicability of genomic data as a reference for future studies on genetic diversity, taxonomy, evolution and phylogeny of the Acrocomia genus, as well as to support strategies for the conservation, exploration and breeding of Acrocomia species and in particular A. aculeata.

scholarly journals Genetic diversity and phylogeography of the rare riparian moss Dichelyma capillaceum (With.) Myr. inferred from trnL-F plastid DNA sequences

2009 ◽  
Vol 61 (1) ◽  
pp. 85-91 ◽  
Author(s):  
M. Sabovljevic ◽  
J.P. Frahm
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
North American ◽  
Genetic Relationships ◽  
Plastid Dna ◽  
Molecular Data ◽  
Genetic Distances ◽  
German Population ◽  
F Region

The genetic relationships of Dichelyma capillaceum (With.) Myr. are studied from chloroplast sequences of the trnL-F region. On the basis of the molecular data obtained, the German population can be considered to be derived from Scandinavian(Swedish) rather than North American populations. To judge from the genetic distances between the Swedish and German populations, the separation must have occurred along time ago.

scholarly journals Genomic diversity of 39 Pyropia species grown in Japan

2020 ◽  
Author(s):  
Yukio Nagano ◽  
Kei Kimura ◽  
Genta Kobayashi ◽  
Yoshio Kawamura
Keyword(s):  
Genetic Diversity ◽  
Genetic Heterogeneity ◽  
Genome Analysis ◽  
Nuclear Genome ◽  
Genomic Diversity ◽  
Mitochondrial Genomes ◽  
Similar Group ◽  
Genome Sequences ◽  
Single Strain ◽  
Organellar Genomes

AbstractPyropia species, such as nori (P. yezoensis), are important marine crops. We analysed the genome sequences of 39 Pyropia species grown in Japan. Analysis of organellar genomes showed that the chloroplast and mitochondrial genomes of P. yezoensis have different evolutionary histories. The genetic diversity of Japanese P. yezoensis used in this study is lower than that of Chinese wild P. yezoensis. Nuclear genome analysis revealed nearly 90% of the analysed loci as allodiploid in the hybrid between P. yezoensis and P. tenera. Although the genetic diversity of Japanese P. yezoensis is low, analysis of nuclear genomes genetically separated each accession. Accessions isolated from the sea were often genetically similar to those being farmed. Study of genetic heterogeneity within a single strain of P. yezoensis showed that accessions with frequent abnormal budding formed a single, genetically similar group. The results will be useful for breeding and the conservation of Pyropia species.

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