Protective Vaccination against Papillomavirus-Induced Skin Tumors under Immunocompetent and Immunosuppressive Conditions: A Preclinical Study Using a Natural Outbred Animal Model

The aim of this study is the accurate detection of acetaminophen (AP) for point-of-care (POC) clinical diagnosis. The concentration of acetaminophen was measured in over-the-counter pharmaceutical tablets and in serum samples taken from mice.

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Correction: Sonochemical synthesis of polyoxometalate-stabilized gold nanoparticles for point-of-care determination of acetaminophen levels: preclinical study in an animal model

RSC Advances ◽

10.1039/d0ra90056g ◽

2020 ◽

Vol 10 (31) ◽

pp. 18138-18138

Author(s):

Tahereh Rohani Bastami ◽

Abolphazl Ghaedi ◽

Scott G. Mitchell ◽

Aida Javadian-Saraf ◽

Mohammad Karimi

Keyword(s):

Gold Nanoparticles ◽

Animal Model ◽

Point Of Care ◽

Preclinical Study ◽

Sonochemical Synthesis

Correction for ‘Sonochemical synthesis of polyoxometalate-stabilized gold nanoparticles for point-of-care determination of acetaminophen levels: preclinical study in an animal model’ by Tahereh Rohani Bastami et al., RSC Adv., 2020, 10, 16805–16816, DOI: 10.1039/D0RA00931H.

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Understanding the role of stem cells in urinary bladder regeneration - a preclinical study in a large animal model

European Urology Supplements ◽

10.1016/s1569-9056(17)30376-7 ◽

2017 ◽

Vol 16 (3) ◽

pp. e537 ◽

Cited By ~ 1

Author(s):

M. Pokrywczynska ◽

A. Jundzill ◽

M. Buhl ◽

D. Balcerczyk ◽

M. Rasmus ◽

...

Keyword(s):

Stem Cells ◽

Animal Model ◽

Urinary Bladder ◽

Preclinical Study ◽

Large Animal Model ◽

Large Animal ◽

Bladder Regeneration

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A Novel Biodegradable Pancreatic Stent for Human Pancreatic Applications: A Preclinical Study in a Large Animal Model

Gastrointestinal Endoscopy ◽

10.1016/j.gie.2006.03.036 ◽

2006 ◽

Vol 63 (5) ◽

pp. AB84

Author(s):

Johanna M. Laukkarinen ◽

Teemu Lamsa ◽

Isto Nordback ◽

Joonas Mikkonen ◽

Juhani Sand

Keyword(s):

Animal Model ◽

Preclinical Study ◽

Large Animal Model ◽

Large Animal ◽

Pancreatic Stent

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Profil Reproduksi Jantan Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Stadia Muda, Pradewasa, dan Dewasa

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.429 ◽

2018 ◽

Vol 7 (1) ◽

pp. 29-36

Author(s):

Laksmindra Fitria ◽

Mulyati Muyati ◽

Cut M. Tiraya ◽

Andreas S. Budi

Keyword(s):

Animal Model ◽

Body Mass ◽

Testosterone Level ◽

Reproductive System ◽

Wistar Rats ◽

Sperm Concentration ◽

Preclinical Study ◽

Reproductive Age ◽

Normal Male ◽

Sperm Analysis

Reproductive biology is one of prominent studies in biomedical research. Disruption in reproductive system becomes a major problem both in humans and animals. Preclinical study using animal model is therefore needed to initiate clinical studies and diagnostic purposes. Wistar rats are commonly used for research in male and female reproductive system due to their representation of mammal biological system. For that purpose, their reproductive age must be determined to meet the aim of studies. This research was carried out to provide reproductive profile of normal male Wistar rats from different ages, furthermore categorizing them into three checkpoints: young, subadult, and adult. Variables observed including: body mass, testosterone level, reproductive glands index, spermatogenesis, and sperm analysis. Results demonstrated that body mass, testosterone level, and reproductive glands index increase with age (significant at the age of 6–7 weeks). Spermatogenesis is initiated at the age of 7 weeks, characterized by significant increase in the number of spermatogenic cells which then maintained at subsequent ages. Spermatozoa has been produced at the age of 6 weeks, however still in low concentration, immotile, and not viable. The quantity and quality of sperm also increase with age. At the age of 8–9 weeks, sperm concentration significantly increases, progressive movement occurs, and viability is close to 100 %. In conclusion, rats aged 4–5 weeks can be categorized as young, sexually immature; rats aged 6–7 weeks are subadult, the reproductive system has well-developed (puberty) but spermatozoa are still immotile (infertile); rats aged 8–9 weeks are adult, sexually mature, and ready for mating, thus suitable as animal model for the study of reproductive system.Key words: Wistar rats, reproductive system, testosterone, spermatogenesis, spermatozoa.

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Barriers in Systemic Delivery and Preclinical Testing of Synthetic microRNAs in Animal Models: an Experimental Study on miR-215-5p Mimic

Physiological Research ◽

10.33549/physiolres.934571 ◽

2021 ◽

pp. 481-487

Author(s):

T MACHACKOVA ◽

P VYCHYTILOVA-FALTEJSKOVA ◽

K SOUCKOVA ◽

R LAGA ◽

L ANDROVIČ ◽

...

Keyword(s):

Animal Model ◽

Animal Models ◽

Blood Plasma ◽

Preclinical Study ◽

Human Blood Plasma ◽

High Homology ◽

Systemic Delivery ◽

Specific Expression ◽

Hydroxypropyl Methacrylamide ◽

Colorectal Cancer Cell Lines

Mus musculus is the most commonly used animal model in microRNA research; however, little is known about the endogenous miRNome of the animals used in the miRNA-targeting preclinical studies with the human xenografts. In the presented study, we evaluated the NOD/SCID gamma mouse model for the preclinical study of systemic miR-215-5p substitution with a semitelechelic poly[N-(2-hydroxypropyl)-methacrylamide]-based carrier conjugated with miR-215-5p-mimic via a reductively degradable disulfide bond. Murine mmu-miR-215-5p and human hsa-miR-215-5p have a high homology of mature sequences with only one nucleotide substitution. Due to the high homology of hsa-miR-215-5p and mmu-hsa-miR-215-5p, a similar expression in human and NOD/SCID gamma mice was expected. Expression of mmu-miR-215 in murine organs did not indicate tissue-specific expression and was highly expressed in all examined tissues. All animals included in the study showed a significantly higher concentration of miR-215-5p in the blood plasma compared to human blood plasma, where miR-215-5p is on the verge of a reliable detection limit. However, circulating mmu-miR-215-5p did not enter the human xenograft tumors generated with colorectal cancer cell lines since the levels of miR-215-5p in control tumors remained notably lower compared to those originally transfected with miR-215-5p. Finally, the systemic administration of polymer-miR-215-5p-mimic conjugate to the tail vein did not increase miR-215-5p in NOD/SCID gamma mouse blood plasma, organs, and subcutaneous tumors. It was impossible to distinguish hsa-miR-215-5p and mmu-miR-215-5p in the murine blood and organs due to the high expression of endogenous mmu-miR-215-5p. In conclusion, the examination of endogenous tissue and circulating miRNome of an experimental animal model of choice might be necessary for future miRNA studies focused on the systemic delivery of miRNA-based drugs conducted in the animal models.

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Effects of bioresorbable magnesium‐based materials on bone healing: a preclinical study in a large growing‐animal model

Clinical Oral Implants Research ◽

10.1111/clr.91_13644 ◽

2020 ◽

Vol 31 (S20) ◽

pp. 149-149

Author(s):

Valentin Herber ◽

B Clement ◽

Patrick Holweg ◽

Nicholas Donohue ◽

Uwe Yacine Schwarze ◽

...

Keyword(s):

Animal Model ◽

Bone Healing ◽

Preclinical Study

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H-ras activation in benign and self-regressing skin tumors (keratoacanthomas) in both humans and an animal model system

Molecular and Cellular Biology ◽

10.1128/mcb.8.2.786-793.1988 ◽

1988 ◽

Vol 8 (2) ◽

pp. 786-793

Author(s):

J Leon ◽

H Kamino ◽

J J Steinberg ◽

A Pellicer

Keyword(s):

Animal Model ◽

Model System ◽

Skin Tumors ◽

3T3 Cells ◽

Ras Gene ◽

Ras Genes ◽

Ras Activation ◽

Nih 3T3 ◽

Nih 3T3 Cells ◽

Codon 61

The involvement of the ras oncogenes in tumorigenesis was investigated in keratoacanthomas, which are benign and self-regressing skin tumors, both in humans and in a corresponding animal model system. Keratoacanthomas were induced on rabbit ears by repeated applications of 7,12-dimethylbenz(a)anthracene. About 60% of the tumor DNAs produced transformed foci after transfection into NIH 3T3 cells, and in all of them the transforming gene was identified as H-ras by Southern and Northern (RNA) hybridization. Immunoprecipitation experiments suggested that the transforming rabbit H-ras protein carried a mutation in codon 61. In addition, an activated H-ras gene was detected in a human keratoacanthoma by using a nude mouse tumorigenesis assay after transfection of tumor DNA into NIH 3T3 cells. This is the first report of ras activation in a benign human tumor. The transforming human H-ras gene showed a point mutation in codon 61 that would result in leucine instead of the glutamine present in the normal gene product. The finding of ras activation in tumors that are not only benign but also self-regressing indicates that activated ras genes are not sufficient to maintain a neoplastic phenotype, although they likely play a role in early stages of tumorigenesis.

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