scholarly journals Specificity and Dynamics of Effector and Memory CD8 T Cell Responses in Human Tick-Borne Encephalitis Virus Infection

2015 ◽  
Vol 11 (1) ◽  
pp. e1004622 ◽  
Author(s):  
Kim Blom ◽  
Monika Braun ◽  
Jolita Pakalniene ◽  
Laura Dailidyte ◽  
Vivien Béziat ◽  
...  
2010 ◽  
Vol 84 (17) ◽  
pp. 8743-8752 ◽  
Author(s):  
Karen Baur ◽  
Kay Brinkmann ◽  
Marc Schweneker ◽  
Juliane Pätzold ◽  
Christine Meisinger-Henschel ◽  
...  

ABSTRACT Efficient T-cell responses against recombinant antigens expressed by vaccinia virus vectors require expression of these antigens in the early phase of the virus replication cycle. The kinetics of recombinant gene expression in poxviruses are largely determined by the promoter chosen. We used the highly attenuated modified vaccinia virus Ankara (MVA) to determine the role of promoters in the induction of CD8 T-cell responses. We constructed MVA recombinants expressing either enhanced green fluorescent protein (EGFP) or chicken ovalbumin (OVA), each under the control of a hybrid early-late promoter (pHyb) containing five copies of a strong early element or the well-known early-late p7.5 or pS promoter for comparison. In primary or cultured cells, EGFP expression under the control of pHyb was detected within 30 min, as an immediate-early protein, and remained higher over the first 6 h of infection than p7.5- or pS-driven EGFP expression. Repeated immunizations of mice with recombinant MVA expressing OVA under the control of the pHyb promoter led to superior acute and memory CD8 T-cell responses compared to those to p7.5- and pS-driven OVA. Moreover, OVA expressed under the control of pHyb replaced the MVA-derived B8R protein as the immunodominant CD8 T-cell antigen after three or more immunizations. This is the first demonstration of an immediate-early neoantigen expressed by a poxviral vector resulting in superior induction of neoantigen-specific CD8 T-cell responses.


2007 ◽  
Vol 178 (4) ◽  
pp. 2028-2037 ◽  
Author(s):  
Parisa Sinai ◽  
Rance E. Berg ◽  
J. Marshall Haynie ◽  
Merrill J. Egorin ◽  
Robert L. Ilaria ◽  
...  

2006 ◽  
Vol 177 (1) ◽  
pp. 450-458 ◽  
Author(s):  
Michael W. Munks ◽  
Kathy S. Cho ◽  
Amelia K. Pinto ◽  
Sophie Sierro ◽  
Paul Klenerman ◽  
...  

2010 ◽  
Vol 185 (3) ◽  
pp. 1429-1441 ◽  
Author(s):  
Abi G. Aleyas ◽  
Young Woo Han ◽  
Junu A. George ◽  
Bumseok Kim ◽  
Koanhoi Kim ◽  
...  

Blood ◽  
2012 ◽  
Vol 119 (4) ◽  
pp. 967-977 ◽  
Author(s):  
Agostinho Carvalho ◽  
Antonella De Luca ◽  
Silvia Bozza ◽  
Cristina Cunha ◽  
Carmen D'Angelo ◽  
...  

Abstract Aspergillus fumigatus is a model fungal pathogen and a common cause of severe infections and diseases. CD8+ T cells are present in the human and murine T-cell repertoire to the fungus. However, CD8+ T-cell function in infection and the molecular mechanisms that control their priming and differentiation into effector and memory cells in vivo remain elusive. In the present study, we report that both CD4+ and CD8+ T cells mediate protective memory responses to the fungus contingent on the nature of the fungal vaccine. Mechanistically, class I MHC-restricted, CD8+ memory T cells were activated through TLR3 sensing of fungal RNA by cross-presenting dendritic cells. Genetic deficiency of TLR3 was associated with susceptibility to aspergillosis and concomitant failure to activate memory-protective CD8+ T cells both in mice and in patients receiving stem-cell transplantations. Therefore, TLR3 essentially promotes antifungal memory CD8+ T-cell responses and its deficiency is a novel susceptibility factor for aspergillosis in high-risk patients.


Immunity ◽  
2013 ◽  
Vol 38 (4) ◽  
pp. 742-753 ◽  
Author(s):  
Jan C. Dudda ◽  
Bruno Salaun ◽  
Yun Ji ◽  
Douglas C. Palmer ◽  
Gwennaelle C. Monnot ◽  
...  

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